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Species: Homo sapiens
Genetic Insert: F-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32527825
Proper citation: RRID:Addgene_155221 Copy
Species: Homo sapiens
Genetic Insert: F-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32527825
Proper citation: RRID:Addgene_155222 Copy
Species: Homo sapiens
Genetic Insert: Mutated version of F-tractin (aa 15-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32527825
Proper citation: RRID:Addgene_155223 Copy
Species: Homo sapiens
Genetic Insert: F-tractin (aa9-52 of rat ITPKA) fused to mCitrine C-term tagged with codon optimized (EAAAR)x8 and CaaX seq derived from Kras4b
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32527825
Proper citation: RRID:Addgene_155224 Copy
Vector Backbone Description: Backbone Size:9512; Vector Backbone:Addgene #155182; Vector Types:Mammalian Expression, Unspecified, Piggybac transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35567999
Comments: The T2A-Puro sequence was re-amplified and cloned into the backbone to remove BbsI restriction site in the T2A sequence. To clone Cas13d gRNA for gene knockdown, digest the plasmid with BbsI and then ligate annealed oligo into the backbone. gRNA could be designed using https://cas13design.nygenome.org/ and annealing oligoes should have the following format, where Ns and Ms are the gRNA sequence and its reverse-complement counterpart:
FWD:5'- AAACNNNNNNNNNNNNN
RVS: 5'-AAAAMMMMMMMMMMM
Proper citation: RRID:Addgene_155184 Copy
Species: Homo sapiens
Genetic Insert: Cas13d RUNX1 gRNA2
Vector Backbone Description: Backbone Size:9817; Vector Backbone:Addgene plasmid #155184; Vector Types:Mammalian Expression, Piggybac transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35567999
Proper citation: RRID:Addgene_155186 Copy
Species: Homo sapiens
Genetic Insert: Cas13d SOX17 gRNA1
Vector Backbone Description: Backbone Size:9817; Vector Backbone:Addgene plasmid #155184; Vector Types:Mammalian Expression, Piggybac transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35567999
Proper citation: RRID:Addgene_155187 Copy
Species: Homo sapiens
Genetic Insert: F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32527825
Proper citation: RRID:Addgene_155220 Copy
Species: Homo sapiens
Genetic Insert: CasRx
Vector Backbone Description: Backbone Size:6487; Vector Backbone:Addgene #154398; Vector Types:Mammalian Expression, Unspecified, Piggybac transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35567999
Proper citation: RRID:Addgene_155183 Copy
Species: Homo sapiens
Genetic Insert: PA1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17500065
Proper citation: RRID:Addgene_15559 Copy
Species: Homo sapiens
Genetic Insert: DPY30
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pWZL neo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17500065
Comments: FLAG-tagged hDPY-30 was cloned by PCR from HeLaS cDNA using primers 5'-C AGT AGG ATC CTG GCC ACC ATG GAC TAC AAG GAC GAC GAT GAC AAG ATG GAG CCA GAG CAG ATG CTG G-3' and 5'-TCG CTG CTC GAG TCA GTT TCG ATC TTC AAA CTG TGC-3'.
Proper citation: RRID:Addgene_15555 Copy
Species: Mus musculus
Genetic Insert: Wdr5
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4972; Vector Backbone:pGEX-5X1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17500065
Proper citation: RRID:Addgene_15553 Copy
Species: Mus musculus
Genetic Insert: Wdr5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17500065
Proper citation: RRID:Addgene_15552 Copy
Species: Homo sapiens
Genetic Insert: RbBP5
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4972; Vector Backbone:pGEX-5X1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17500065
Proper citation: RRID:Addgene_15551 Copy
Species: Homo sapiens
Genetic Insert: RbBP5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17500065
Proper citation: RRID:Addgene_15550 Copy
Species: Homo sapiens
Genetic Insert: YTHDF3(1-385)
Vector Backbone Description: Backbone Marker:New England Biolabs; Vector Backbone:pSNAPf; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32451507
Proper citation: RRID:Addgene_155353 Copy
Species: Homo sapiens
Genetic Insert: YTHDF1(264-559)
Vector Backbone Description: Vector Backbone:pFUGW-CRY2olig-BFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32451507
Proper citation: RRID:Addgene_155354 Copy
Genetic Insert: cry2olig-BFP
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32451507
Proper citation: RRID:Addgene_155355 Copy
Species: Homo sapiens
Genetic Insert: YTHDF1 (1-359)
Vector Backbone Description: Backbone Marker:New England Biolabs; Vector Backbone:pSNAPf; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32451507
Proper citation: RRID:Addgene_155351 Copy
Species: Homo sapiens
Genetic Insert: htt 25Q Delta Pro
Vector Backbone Description: Backbone Size:5800; Vector Backbone:p416 GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832050
Comments: Figure 2, III in article.
Lindquist lab number: 7536
Proper citation: RRID:Addgene_15568 Copy
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