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Genetic Insert: SUMO-sfGFP-WT
Vector Backbone Description: Backbone Size:4807; Vector Backbone:pALS3; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Comments: This plasmid encodes expression of a) sfGFP-WT, uninterrupted by TAG codons, and b) Methanomethylophilus alvus tRNA(6). It is to be paired with a plasmid that expresses M. alvus pyrrolysyl-tRNA synthetase (MaRS). This control plasmid is used during fluorescence-based selection and characterization of one or more MaRS mutants. It is distinguished from a previous Ma version by containing a tetracycline efflux marker.
Proper citation: RRID:Addgene_212119 Copy
Species: Synthetic
Genetic Insert: ChR2-mCherry
Vector Backbone Description: Vector Backbone:pAAV-hDlx-Flex-dTomato-Fishell_7; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41565808
Comments: Please visit https://doi.org/10.1101/2024.07.20.604399 for bioRxiv preprint.
Proper citation: RRID:Addgene_224459 Copy
Genetic Insert: SUMO-sfGFP-WT
Vector Backbone Description: Backbone Size:4807; Vector Backbone:pALS3; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Comments: This plasmid encodes expression of a) sumo-sfGFP, uninterrupted by TAG codons, and b) Methanomethylophilus alvus pyrrolysine tRNA(6). It is to be paired with a plasmid that expresses M. alvus pyrrolysyl-tRNA synthetase (MaRS). This pALS3 control plasmid is used during fluorescence-based selection and characterization of Ma RS mutants. The plasmid here is distinguished from a previous Ma version by the presence of a tetracycline efflux marker.
Proper citation: RRID:Addgene_212122 Copy
Genetic Insert: SUMO-TAG35-sfGFP
Vector Backbone Description: Backbone Size:4807; Vector Backbone:pALS3; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Comments: This plasmid encodes expression of a) SUMO-sfGFP with an amber (TAG) codon at E35 within the SUMO coding region and b) Methanomethylophilus alvus tRNA(6). It is to be paired with a plasmid that expresses an M. alvus pyrrolysyl-tRNA synthetase (MaRS). This plasmid is used during fluorescence-based selection and characterization of one or more MaRS mutants. It is distinguished from the previous Ma version by containing a tetracycline efflux marker. The SUMO linker is included in order to distance the TAG site from sfGFP.
Proper citation: RRID:Addgene_212120 Copy
Species: Homo sapiens
Genetic Insert: PSMB5
Vector Backbone Description: Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:38600324
Proper citation: RRID:Addgene_217896 Copy
Species: Synthetic
Genetic Insert: CeNL Nanolantern
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39252965
Proper citation: RRID:Addgene_208839 Copy
Species: Homo sapiens
Genetic Insert: RNF149
Vector Backbone Description: Vector Backbone:pLOC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36610398
Proper citation: RRID:Addgene_225332 Copy
Species: Synthetic
Genetic Insert: CeNLuc LumiFluor
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39252965
Proper citation: RRID:Addgene_208838 Copy
Species: Homo sapiens
Genetic Insert: M6PR
Vector Backbone Description: Vector Backbone:pBeta-Actin-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38598291
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
Proper citation: RRID:Addgene_224407 Copy
Species: Homo sapiens
Genetic Insert: CD28 CAR hinge (I114 – P152)
Vector Backbone Description: Backbone Size:4984; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39217198
Proper citation: RRID:Addgene_224929 Copy
Species: Synthetic
Genetic Insert: GeNL Nanolantern
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39252965
Proper citation: RRID:Addgene_208841 Copy
Species: Synthetic
Genetic Insert: GCaMP8m
Vector Backbone Description: Backbone Size:4093; Vector Backbone:pAAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40623068
Comments: Please visit https://doi.org/10.1101/2022.01.22.477348 for bioRxiv preprint.
Proper citation: RRID:Addgene_210400 Copy
Species: Homo sapiens
Genetic Insert: PD-1
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral, Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29996099
Comments: Plasmid lab code: L610
Proper citation: RRID:Addgene_223573 Copy
Species: Homo sapiens
Genetic Insert: CD80
Vector Backbone Description: Vector Backbone:pPPI4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31757674
Comments: Plasmid lab code: L485
Proper citation: RRID:Addgene_223579 Copy
Species: Homo sapiens
Genetic Insert: PD-L1
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral, Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29996099
Comments: Plasmid lab code: L638
Proper citation: RRID:Addgene_223577 Copy
Species: Homo sapiens
Genetic Insert: CD28
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral, Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37160118
Comments: Plasmid lab code: L1683
Proper citation: RRID:Addgene_223610 Copy
Species: Synthetic
Genetic Insert: Antares
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39252965
Proper citation: RRID:Addgene_208852 Copy
Species: Homo sapiens
Genetic Insert: B3GNT5
Vector Backbone Description: Vector Backbone:lentiGuide-neo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37577488
Comments: Please visit https://doi.org/10.1101/2023.08.02.551727 for bioRxiv preprint.
Proper citation: RRID:Addgene_220867 Copy
Species: Homo sapiens
Genetic Insert: CD86
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral, Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37160118
Comments: Plasmid lab code: L2644
Proper citation: RRID:Addgene_223618 Copy
Species: Homo sapiens
Genetic Insert: PD-L1
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral, Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37160118
Comments: Plasmid lab code: L2646
Proper citation: RRID:Addgene_223619 Copy
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