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Species: Rattus norvegicus
Genetic Insert: aPKC
Vector Backbone Description: Vector Backbone:pCS2+ hsp70; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25600237
Proper citation: RRID:Addgene_105946 Copy
Species: Homo sapiens
Genetic Insert: Cep152
Vector Backbone Description: Vector Backbone:pCS2+ hsp70; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25600237
Proper citation: RRID:Addgene_105943 Copy
Species: Danio rerio
Genetic Insert: Centrin
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25600237
Proper citation: RRID:Addgene_105941 Copy
Species: Homo sapiens
Genetic Insert: PCNA
Vector Backbone Description: Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25600237
Proper citation: RRID:Addgene_105942 Copy
Species: Mus musculus
Genetic Insert: Vmn1r105
Vector Backbone Description: Backbone Size:3015; Vector Backbone:pGEM-T Easy; Vector Types:to generate riboprobe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27243442
Comments: 99% identical (10 nt mismatches)
Linearize with SacII, use SP6 RNA polymerase for IVT.
Proper citation: RRID:Addgene_105994 Copy
Species: Mus musculus
Genetic Insert: Plcb2
Vector Backbone Description: Backbone Size:3015; Vector Backbone:pGEM-T Easy; Vector Types:to generate riboprobe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27243442
Comments: Linearize with SacII, use SP6 RNA polymerase for IVT.
Proper citation: RRID:Addgene_105991 Copy
Species: Staphylococcus aureus
Genetic Insert: Cas9 nuclease
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29334369
Comments: Addgene NGS results show a C-terminal HA tag rather than 3x HA tag. Also, the mCD4 selection marker matches NCBI reference NP_038516.1 and contains residues 1-427. Plasmids should function as described in the associated publication.
Proper citation: RRID:Addgene_105998 Copy
Species: Homo sapiens
Genetic Insert: Opto-GPR1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29769519
Comments: two step cloning; 5' and 3' sites cannot be used for (sub)cloning
Proper citation: RRID:Addgene_105999 Copy
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression, Lentiviral, CRISPR, S.pyogenes sgRNA expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29334369
Comments: Dual sgRNA vector to express one sgRNA for S.pyogenes Cas9 and one sgRNA for S.aureus Cas9. Cloning is done in two steps: 1.) AarI digest to clone the S.pyogenes sgRNA and after preparation of the successfully cloned plasmid 2.) BfuAI digest followed by cloning of the S.aureus sgRNA.
Proper citation: RRID:Addgene_105997 Copy
Species: Homo sapiens
Genetic Insert: Opto-GPR68
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29769519
Comments: two step cloning; 5' and 3' sites cannot be used for (sub)cloning
Proper citation: RRID:Addgene_106031 Copy
Species: Homo sapiens
Genetic Insert: Opto-GPR61
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29769519
Comments: two step cloning; 5' and 3' sites cannot be used for (sub)cloning
Proper citation: RRID:Addgene_106027 Copy
Species: Danio rerio
Genetic Insert: Kif5a
Vector Backbone Description: Vector Backbone:GW_pDONR_P2r-P3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26879757
Proper citation: RRID:Addgene_105973 Copy
Species: Homo sapiens
Genetic Insert: dynactin1-811
Vector Backbone Description: Vector Backbone:GWpTolDest(R4-R2)pA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26879757
Comments: The depositor notes that the L287M found in dynactin is not of functional concern.
Proper citation: RRID:Addgene_105970 Copy
Species: Homo sapiens
Genetic Insert: Opto-GPR42
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29769519
Comments: two step cloning; 5' and 3' sites cannot be used for (sub)cloning
Proper citation: RRID:Addgene_106022 Copy
Species: Rattus norvegicus
Genetic Insert: LAP2b
Vector Backbone Description: Vector Backbone:GW_pDONR_P2r-P3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30120238
Proper citation: RRID:Addgene_105978 Copy
Species: Danio rerio
Genetic Insert: Paxillin
Vector Backbone Description: Vector Backbone:GWpCS2 Dest(R1-R3); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28372636
Comments: The depositor notes that the V94A and P149S mutations found in Paxillin are not of functional concern.
Proper citation: RRID:Addgene_105974 Copy
Species: Mus musculus
Genetic Insert: TADA1-HFD (140-230)
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316427
Proper citation: RRID:Addgene_105984 Copy
Species: Homo sapiens
Genetic Insert: hSyn-Igkappa-myc-FAPdL5-POST-T2A-dTomato-WPRE-bGH
Vector Backbone Description: Backbone Marker:PENN vector core; Backbone Size:4643; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31548370
Proper citation: RRID:Addgene_105981 Copy
Species: Homo sapiens
Genetic Insert: Opto-GPR84
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29769519
Comments: two step cloning; 5' and 3' sites cannot be used for (sub)cloning
Proper citation: RRID:Addgene_106036 Copy
Species: Homo sapiens
Genetic Insert: FLEX-FAPdL5-POST-T2A-dTomato.FLEX
Vector Backbone Description: Backbone Size:4894; Vector Backbone:pAAV-FLEX; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31548370
Proper citation: RRID:Addgene_105982 Copy
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