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Species: Mus musculus
Genetic Insert: Magoh
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4400; Vector Backbone:pCMV-Sport6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20364144
Comments: AluI digestion generates 341 bp, 189 bp, 112 bp. Wt only generates 341 bp +300 bp fragments.
Proper citation: RRID:Addgene_30481 Copy
Species: Homo sapiens
Genetic Insert: HDAC6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14675537
Proper citation: RRID:Addgene_30484 Copy
Species: Homo sapiens
Genetic Insert: HDAC6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14675537
Proper citation: RRID:Addgene_30483 Copy
Species: Homo sapiens
Genetic Insert: HDAC4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_30486 Copy
Genetic Insert: SP146/gp91/GP91/cHS4
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21544095
Comments: From the paper (note that some of these features may not be in this plasmid): All vectors were based on the pRRL-cPPT-hPGK-mcs-WPRE vector, derived from the pRRL-cPPT-hPGKeGFP-WPRE construct. The gp91promoter is the natural myeloid-specific promoter. The CMVenh comes from the peGFP-N1(Clontech, Saint-Germain-en-Laye, France),
the synthetic intron from the pMG5 and the EF1a intron from the pWPI vector http://www.addgene.org/12254 The myeloid-specific enhancer SP107 and SP146 were provided by Senlin Li (Department of Medicine, UTHSCSA, San Antonio, TX, USA). The cHS4 insulator corresponds to the chicken beta-globin 5’-DNase I-hypersensitive
site and was provided by Patrick Salmon (Department of Neuroscience,
Faculty of Medicine, University of Geneva, Switzerland).
Proper citation: RRID:Addgene_30480 Copy
Species: Homo sapiens
Genetic Insert: p300
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCMVb; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_30489 Copy
Species: bacteriophage P1
Genetic Insert: Cre recombinase
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pCSGFP2; Vector Types:Mammalian Expression, Cre/Lox, heat shock inducible; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19266305
Comments: Cre driven by Xenopus laevis heat shock 70 (hsp70) promoter
Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.
Proper citation: RRID:Addgene_30524 Copy
Genetic Insert: CMVenh/gp91/eGFP/cHS4
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21544095
Comments: From the paper (note that some of these features may not be in this plasmid): All vectors were based on the pRRL-cPPT-hPGK-mcs-WPRE vector, derived from the pRRL-cPPT-hPGKeGFP-WPRE construct. The gp91promoter is the natural myeloid-specific promoter. The CMVenh comes from the peGFP-N1(Clontech, Saint-Germain-en-Laye, France),
the synthetic intron from the pMG5 and the EF1a intron from the pWPI vector http://www.addgene.org/12254 The myeloid-specific enhancer SP107 and SP146 were provided by Senlin Li (Department of Medicine, UTHSCSA, San Antonio, TX, USA). The cHS4 insulator corresponds to the chicken beta-globin 5’-DNase I-hypersensitive
site and was provided by Patrick Salmon (Department of Neuroscience,
Faculty of Medicine, University of Geneva, Switzerland).
Proper citation: RRID:Addgene_30471 Copy
Genetic Insert: SP107/gp91/eGFP
Vector Backbone Description: Backbone Size:6800; Vector Backbone:pRRL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21544095
Comments: From the paper (note that some of these features may not be in this plasmid): All vectors were based on the pRRL-cPPT-hPGK-mcs-WPRE vector, derived from the pRRL-cPPT-hPGKeGFP-WPRE construct. The gp91promoter is the natural myeloid-specific promoter. The CMVenh comes from the peGFP-N1(Clontech, Saint-Germain-en-Laye, France),
the synthetic intron from the pMG5 and the EF1a intron from the pWPI vector http://www.addgene.org/12254 The myeloid-specific enhancer SP107 and SP146 were provided by Senlin Li (Department of Medicine, UTHSCSA, San Antonio, TX, USA). The cHS4 insulator corresponds to the chicken beta-globin 5’-DNase I-hypersensitive
site and was provided by Patrick Salmon (Department of Neuroscience,
Faculty of Medicine, University of Geneva, Switzerland).
Proper citation: RRID:Addgene_30473 Copy
Genetic Insert: SP146/gp47
Vector Backbone Description: Backbone Size:6800; Vector Backbone:pRRL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21544095
Comments: From the paper (note that some of these features may not be in this plasmid): All vectors were based on the pRRL-cPPT-hPGK-mcs-WPRE vector, derived from the pRRL-cPPT-hPGKeGFP-WPRE construct. The gp91promoter is the natural myeloid-specific promoter. The CMVenh comes from the peGFP-N1(Clontech, Saint-Germain-en-Laye, France),
the synthetic intron from the pMG5 and the EF1a intron from the pWPI vector http://www.addgene.org/12254 The myeloid-specific enhancer SP107 and SP146 were provided by Senlin Li (Department of Medicine, UTHSCSA, San Antonio, TX, USA). The cHS4 insulator corresponds to the chicken beta-globin 5’-DNase I-hypersensitive
site and was provided by Patrick Salmon (Department of Neuroscience,
Faculty of Medicine, University of Geneva, Switzerland).
Proper citation: RRID:Addgene_30475 Copy
Genetic Insert: SP146/gp91/eGFP
Vector Backbone Description: Backbone Size:6800; Vector Backbone:pRRL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21544095
Comments: From the paper (note that some of these features may not be in this plasmid): All vectors were based on the pRRL-cPPT-hPGK-mcs-WPRE vector, derived from the pRRL-cPPT-hPGKeGFP-WPRE construct. The gp91promoter is the natural myeloid-specific promoter. The CMVenh comes from the peGFP-N1(Clontech, Saint-Germain-en-Laye, France),
the synthetic intron from the pMG5 and the EF1a intron from the pWPI vector http://www.addgene.org/12254 The myeloid-specific enhancer SP107 and SP146 were provided by Senlin Li (Department of Medicine, UTHSCSA, San Antonio, TX, USA). The cHS4 insulator corresponds to the chicken beta-globin 5’-DNase I-hypersensitive
site and was provided by Patrick Salmon (Department of Neuroscience,
Faculty of Medicine, University of Geneva, Switzerland).
Proper citation: RRID:Addgene_30474 Copy
Genetic Insert: SP146/gp91/eGFP/cHS4
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21544095
Comments: From the paper (note that some of these features may not be in this plasmid): All vectors were based on the pRRL-cPPT-hPGK-mcs-WPRE vector, derived from the pRRL-cPPT-hPGKeGFP-WPRE construct. The gp91promoter is the natural myeloid-specific promoter. The CMVenh comes from the peGFP-N1(Clontech, Saint-Germain-en-Laye, France),
the synthetic intron from the pMG5 and the EF1a intron from the pWPI vector http://www.addgene.org/12254 The myeloid-specific enhancer SP107 and SP146 were provided by Senlin Li (Department of Medicine, UTHSCSA, San Antonio, TX, USA). The cHS4 insulator corresponds to the chicken beta-globin 5’-DNase I-hypersensitive
site and was provided by Patrick Salmon (Department of Neuroscience,
Faculty of Medicine, University of Geneva, Switzerland).
Proper citation: RRID:Addgene_30476 Copy
Genetic Insert: CMVenh/gp91/GP91
Vector Backbone Description: Backbone Size:6800; Vector Backbone:pRRL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21544095
Comments: From the paper (note that some of these features may not be in this plasmid): All vectors were based on the pRRL-cPPT-hPGK-mcs-WPRE vector, derived from the pRRL-cPPT-hPGKeGFP-WPRE construct. The gp91promoter is the natural myeloid-specific promoter. The CMVenh comes from the peGFP-N1(Clontech, Saint-Germain-en-Laye, France),
the synthetic intron from the pMG5 and the EF1a intron from the pWPI vector http://www.addgene.org/12254 The myeloid-specific enhancer SP107 and SP146 were provided by Senlin Li (Department of Medicine, UTHSCSA, San Antonio, TX, USA). The cHS4 insulator corresponds to the chicken beta-globin 5’-DNase I-hypersensitive
site and was provided by Patrick Salmon (Department of Neuroscience,
Faculty of Medicine, University of Geneva, Switzerland).
Proper citation: RRID:Addgene_30478 Copy
Species: Mus musculus
Genetic Insert: Bmi1
Vector Backbone Description: Backbone Size:4918; Vector Backbone:pCS2+ att-cherry-HA (C-term cherry-HA); Vector Types:Mammalian Expression, Xenopus/Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21552206
Proper citation: RRID:Addgene_30516 Copy
Species: Mus musculus
Genetic Insert: macroH2A1.2-C
Vector Backbone Description: Vector Backbone:pDEST pCS2+ att-eGFP-HA (C term eGFP-HA); Vector Types:Mammalian Expression, Xenopus/Zebrafish expression.; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21552206
Proper citation: RRID:Addgene_30515 Copy
Species: Mus musculus
Genetic Insert: mH2A2 shRNA
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:6343; Vector Backbone:pSUPER retro puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21552206
Comments: mH2A2 shRNA: 5’-GCAGAGGACAAGAAGCTTAAA
Proper citation: RRID:Addgene_30518 Copy
Species: Mus musculus
Genetic Insert: mH2A1 shRNA
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:6343; Vector Backbone:pSUPER retro puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21552206
Comments: H2A1 shRNA: 5’-GGGTGCAGACAAATGTGAA
Proper citation: RRID:Addgene_30517 Copy
Species: Xenopus laevis
Genetic Insert: HNF1a promoter
Vector Backbone Description: Backbone Size:3812; Vector Backbone:pCSGFP2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10585563
Comments: The GFP variant in the plasmid backbone, pCSGFP2, contains the F64L and the S65T mutations resulting in 35 times brighter green emission (as described in Ikawa M et al., Curr. Top. Dev. Biol. 44 (1999), pp. 1–20)
This construct is derived from the (−6 kb/−58)-GFP construct [Addgene plasmid number 30460] by replacing the EcoRI–HindIII fragment (−494 to −58) with the corresponding EcoRI–HindIII fragment (−494 to −207) of the 3Δ207 clone (as described in Zapp D et al., Mol. Cell. Biol. 13 (1993), pp. 6416–6426).
Proper citation: RRID:Addgene_30462 Copy
Genetic Insert: CMVenh/gp91/EF1aIntron/eGFP
Vector Backbone Description: Backbone Size:6800; Vector Backbone:pRRL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21544095
Comments: From the paper (note that some of these features may not be in this plasmid): All vectors were based on the pRRL-cPPT-hPGK-mcs-WPRE vector, derived from the pRRL-cPPT-hPGKeGFP-WPRE construct. The gp91promoter is the natural myeloid-specific promoter. The CMVenh comes from the peGFP-N1(Clontech, Saint-Germain-en-Laye, France),
the synthetic intron from the pMG5 and the EF1a intron from the pWPI vector http://www.addgene.org/12254 The myeloid-specific enhancer SP107 and SP146 were provided by Senlin Li (Department of Medicine, UTHSCSA, San Antonio, TX, USA). The cHS4 insulator corresponds to the chicken beta-globin 5’-DNase I-hypersensitive
site and was provided by Patrick Salmon (Department of Neuroscience,
Faculty of Medicine, University of Geneva, Switzerland).
Proper citation: RRID:Addgene_30466 Copy
Genetic Insert: CMVenh/gp91/EGFP
Vector Backbone Description: Backbone Size:6800; Vector Backbone:pRRL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21544095
Comments: From the paper (note that some of these features may not be in this plasmid): All vectors were based on the pRRL-cPPT-hPGK-mcs-WPRE vector, derived from the pRRL-cPPT-hPGKeGFP-WPRE construct. The gp91promoter is the natural myeloid-specific promoter. The CMVenh comes from the peGFP-N1(Clontech, Saint-Germain-en-Laye, France),
the synthetic intron from the pMG5 and the EF1a intron from the pWPI vector http://www.addgene.org/12254 The myeloid-specific enhancer SP107 and SP146 were provided by Senlin Li (Department of Medicine, UTHSCSA, San Antonio, TX, USA). The cHS4 insulator corresponds to the chicken beta-globin 5’-DNase I-hypersensitive
site and was provided by Patrick Salmon (Department of Neuroscience,
Faculty of Medicine, University of Geneva, Switzerland).
Proper citation: RRID:Addgene_30468 Copy
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