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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pHS1543 Resource Report Resource Website |
RRID:Addgene_205963 | DinG HNH Cascade with His14-tagged Csf3 | Sulfitobacter sp. JL08, NZ_CP025815 | Ampicillin | PMID:37995242 | Vector Backbone:pET45b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:39:38 | 0 | ||
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pZG61 Resource Report Resource Website |
RRID:Addgene_202575 | HyperADARcd | Drosophila melanogaster | Kanamycin | PMID:34426573 | Genbank: MW026677 | Vector Backbone:pJG054; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin | E488Q | 2026-09-12 02:39:38 | 0 |
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Lenti-EFS-NTID194-GSQ-PMLivaWT-P2A-BLAST Resource Report Resource Website |
RRID:Addgene_208038 | PML (isoform IVa) | Homo sapiens | Ampicillin | PMID:34795231 | For performing SUMO-ID or UbL-ID experiments; PMLiva is excisable using EcoR1-Not1. | Vector Backbone:Derived from Addgene ID# 52962 (Zhang Lab).; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-12 02:39:38 | 0 | |
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TRIPZ-bioGEF-SUMO1nc-PURO Resource Report Resource Website |
RRID:Addgene_208042 | SUMO1nc | Homo sapiens | Ampicillin | PMID:37996419 | Lentiviral packaging of TRIPZ is more efficient with TAT expression (e.g using pcDNA1-TatHIV Addgene ID: 138478) and 2nd generation gag/pol/env combination (e.g. using PSPAX2 Addgene ID: 12260 and pMD2.G Addgene ID: 12259); Use of bioGEF-UBwt may be necessary for certain E3 ligases (e.g. NEDD4 HECT ligase); Use of "non-cleavable" versions may reduce recycling of biotinylated UB and may lead to higher yields of biotinylated protein for proteomic analysis, but induction times should be minimized to avoid toxicity. | Backbone Marker:Open Biosystems; Vector Backbone:TRIPZ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | Q94P Mutation near C-terminus suppresses cleavage by SENPs/DUBs | 2026-09-12 02:39:38 | 0 |
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TRIPZ-bioGEF-SUMO2nc-PURO Resource Report Resource Website |
RRID:Addgene_208043 | SUMO2nc | Homo sapiens | Ampicillin | PMID:37996419 | Lentiviral packaging of TRIPZ is more efficient with TAT expression (e.g using pcDNA1-TatHIV Addgene ID: 138478) and 2nd generation gag/pol/env combination (e.g. using PSPAX2 Addgene ID: 12260 and pMD2.G Addgene ID: 12259); Use of bioGEF-UBwt may be necessary for certain E3 ligases (e.g. NEDD4 HECT ligase); Use of "non-cleavable" versions may reduce recycling of biotinylated UB and may lead to higher yields of biotinylated protein for proteomic analysis, but induction times should be minimized to avoid toxicity. | Backbone Marker:Open Biosystems; Vector Backbone:TRIPZ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | Q90P Mutation near C-terminus suppresses cleavage by SENPs/DUBs | 2026-09-12 02:39:38 | 0 |
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TRIPZ-bioGEF-UBwt-PURO Resource Report Resource Website |
RRID:Addgene_208045 | Ub | Homo sapiens | Ampicillin | PMID:37996419 | Lentiviral packaging of TRIPZ is more efficient with TAT expression (e.g using pcDNA1-TatHIV Addgene ID: 138478) and 2nd generation gag/pol/env combination (e.g. using PSPAX2 Addgene ID: 12260 and pMD2.G Addgene ID: 12259); Use of bioGEF-UBwt may be necessary for certain E3 ligases (e.g. NEDD4 HECT ligase); Use of "non-cleavable" versions may reduce recycling of biotinylated UB and may lead to higher yields of biotinylated protein for proteomic analysis, but induction times should be minimized to avoid toxicity. | Backbone Marker:Open Biosystems; Vector Backbone:TRIPZ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-12 02:39:38 | 0 | |
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pHS1514 Resource Report Resource Website |
RRID:Addgene_205977 | Cas8/Cse1 from MWEE01000013, Cas5-HNH | Candidatus Cloacimonetes bacterium (MAG), MWEE01000013 | Ampicillin | PMID:37995242 | Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:39:39 | 0 | ||
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pCS021 Resource Report Resource Website |
RRID:Addgene_207917 | pU6-AarI-scaffold-term | Arabidopsis thaliana | Ampicillin | PMID:37682951 | Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin | 2026-09-12 02:39:39 | 0 | ||
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pHS1862 Resource Report Resource Website |
RRID:Addgene_205975 | Cas5 from JAGBLH010000220, Cas8-HNH | Selenomonas sp. isolate RGIG9219 Water_deer_Rumen (MAG), JAGBLH010000220 | Ampicillin | PMID:37995242 | Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:39:39 | 0 | ||
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pHS1516 Resource Report Resource Website |
RRID:Addgene_205979 | Cas7 from MWEE01000013, Cas5-HNH | Candidatus Cloacimonetes bacterium (MAG), MWEE01000013 | Ampicillin | PMID:37995242 | Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:39:39 | 0 | ||
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pDRM18 LTN (Luciferase tdTomato Neo) Resource Report Resource Website |
RRID:Addgene_174721 | Firefly Luciferase | Photinus pyralis | Ampicillin | PMID:38001268 | Backbone Size:6685; Vector Backbone:pRRLSIN.cPPT.MND; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin | 2026-09-12 02:39:38 | 0 | ||
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qTAG-N-Blast-mScarlet-ACTB Resource Report Resource Website |
RRID:Addgene_207753 | ACTB Homology Arms flanking a Blast-mScarlet Cassette | Homo sapiens | Ampicillin | PMID:39668248 | To be co-transfected with sgRNA plasmid px330-PITCh-ACTB https://www.addgene.org/207748 . Please visit https://www.biorxiv.org/content/10.1101/2023.11.01.565029v3 for bioRxiv preprint. | Backbone Marker:Invitrogen; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin | 2026-09-12 02:39:39 | 0 | |
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pDRM210 LGP (Luciferase GFP Puro) Resource Report Resource Website |
RRID:Addgene_174722 | Firefly Luciferase | Photinus pyralis | Ampicillin | PMID:38001268 | Backbone Size:6685; Vector Backbone:pRRLSIN.cPPT.MND; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin | 2026-09-12 02:39:38 | 0 | ||
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Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST Resource Report Resource Website |
RRID:Addgene_208046 | RNF4 | Homo sapiens | Ampicillin | PMID:37996419 | For performing BioE3 experiments; can be used for transient expression or packaged into lentiviral particles for stable cell line selection. RNF4wt is excisable using EcoR1-Not1. | Vector Backbone:Derived from Addgene ID# 52962 (Zhang Lab).; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-12 02:39:38 | 0 | |
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Lenti-EFS-BirAopt-GSQ-RNF4ca-P2A-BLAST Resource Report Resource Website |
RRID:Addgene_208047 | RNF4 | Homo sapiens | Ampicillin | PMID:37996419 | Control for RNF4 BioE3 experiments; contains mutations in the conserved RING domain. RNF4ca is excisable using EcoR1-Not1. | Vector Backbone:Derived from Addgene ID# 52962 (Zhang Lab).; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | Cys>Ala mutations in conserved RING domain | 2026-09-12 02:39:38 | 0 |
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pZG164 Resource Report Resource Website |
RRID:Addgene_202583 | HyperADARcd | Drosophila melanogaster | Spectinomycin | PMID:34426573 | Genbank: MW026674 | Vector Backbone:pER8; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin | E488Q | 2026-09-12 02:39:38 | 0 |
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pHS1860 Resource Report Resource Website |
RRID:Addgene_205973 | Cas6/Csy4 from JAGBLH010000220, Cas8-HNH | Selenomonas sp. isolate RGIG9219 Water_deer_Rumen (MAG), JAGBLH010000220 | Ampicillin | PMID:37995242 | Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:39:39 | 0 | ||
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Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST Resource Report Resource Website |
RRID:Addgene_208048 | BirAopt | Synthetic | Ampicillin | PMID:37996419 | Expresses optimized BirA only (with short C-terminal fusion derived from GSQ linker, RBXN polylinker, and P2A remnant). For performing BioE3 experiments; can be used for transient expression or packaged into lentiviral particles for stable cell line selection. | Vector Backbone:Derived from Addgene ID# 52962 (Zhang Lab).; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | BirAopt is human-codon-optimized BirA (PMID 15707685) | 2026-09-12 02:39:38 | 0 |
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pHS1398 Resource Report Resource Website |
RRID:Addgene_205971 | Cas5-HNH crRNA | Candidatus Cloacimonetes bacterium (MAG), MWEE01000013 | Kanamycin | PMID:37995242 | Vector Backbone:pColADuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-09-12 02:39:38 | 0 | ||
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SARS-CoV-2 Mpro protease (R188K mutant - clinical mutant); aliases: 3CLpro, 3C-like Resource Report Resource Website |
RRID:Addgene_204770 | Sars Cov 2 MPRO | Sars Cov 2 | Kanamycin | https://asapdiscovery.org/outputs/target-enabling-packages/ | Vector Backbone:pET28-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | R188K col1 | 2026-09-12 02:39:38 | 0 |
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