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Species: Escherichia coli
Genetic Insert: KanR
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_55750 Copy
Species: Escherichia coli
Genetic Insert: hygromycin B phosphotransferase
Vector Backbone Description: Vector Backbone:pFGL815; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Reference:
Sweigard J, Chumley F, Carroll A, Farrall L, Valent B (1997) A series of vectors for fungal transformation. Fungal Genet Newsl 44: 52-53
Proper citation: RRID:Addgene_58223 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments: AUG --> UUG start codon in lacZ
Proper citation: RRID:Addgene_63861 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments: This plasmid carries a -1 frameshift mutation near the 5' end of lacZ.
Proper citation: RRID:Addgene_63875 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments: This plasmid carries a UAG nonsense mutation near the 5' end of lacZ.
Proper citation: RRID:Addgene_63870 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments: This plasmid carries a UAG nonsense mutation near the 5' end of lacZ.
Proper citation: RRID:Addgene_63871 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments: This plasmid carries a UAA nonsense mutation near the 5' end of lacZ.
Proper citation: RRID:Addgene_63872 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments: This plasmid carries a UAA nonsense mutation near the 5' end of lacZ.
Proper citation: RRID:Addgene_63873 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments: AUG --> GUG start codon in lacZ
Proper citation: RRID:Addgene_63866 Copy
Species: Escherichia coli
Genetic Insert: Translation factor cistrons 22-30
Vector Backbone Description: Backbone Size:5018; Vector Backbone:BioBrick pET-24a(+); Vector Types:Escherichia coli; Bacterial Resistance:Kanamycin
References:
Comments: The plasmid can be prone to recombination. The depositing lab suggests selecting multiple clones for restriction digest analysis as described in the publication prior to proceeding.
Proper citation: RRID:Addgene_117762 Copy
Species: Escherichia coli
Genetic Insert: Translation factor cistrons 14-21
Vector Backbone Description: Backbone Size:5018; Vector Backbone:BioBrick pET-24a(+); Vector Types:Escherichia coli; Bacterial Resistance:Kanamycin
References:
Comments: The plasmid can be prone to recombination. The depositing lab suggests selecting multiple clones for restriction digest analysis as described in the publication prior to proceeding.
Proper citation: RRID:Addgene_117761 Copy
Species: Escherichia coli
Genetic Insert: Translation factor cistrons 1-13
Vector Backbone Description: Backbone Size:5018; Vector Backbone:BioBrick pET-24a(+); Vector Types:Escherichia coli; Bacterial Resistance:Tetracyline and Kanamycin
References:
Comments: The plasmid can be prone to recombination. The depositing lab suggests selecting multiple clones for restriction digest analysis as described in the publication before proceeding.
Proper citation: RRID:Addgene_117760 Copy
Species: Escherichia coli
Genetic Insert: e. coli galactokinase
Vector Backbone Description: Backbone Size:10000; Vector Backbone:PMSG346; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_26958 Copy
Species: Escherichia coli
Genetic Insert: E. coli polyphosphate kinase
Vector Backbone Description: Backbone Marker:K. Melcher; Vector Backbone:pKM263; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_38333 Copy
Species: Escherichia coli
Genetic Insert: E. coli polyphosphate kinase
Vector Backbone Description: Backbone Size:5389; Vector Backbone:pETM-41; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_38334 Copy
Species: Escherichia coli
Genetic Insert: Lon protease fragment
Vector Backbone Description: Vector Backbone:pETM41; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: See "Nomura, K., Kato, J., Takiguchi, N., Ohtake, H., Kuroda, A., 2004. Effects of inorganic polyphosphate on the proteolytic and DNA-binding activities of Lon in Escherichia coli. J. Biol. Chem. 279, 34406–34410." for more information.
Proper citation: RRID:Addgene_38330 Copy
Species: Escherichia coli
Genetic Insert: E. coli polyphosphatase
Vector Backbone Description: Backbone Marker:K. Melcher; Vector Backbone:pKM263; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: See "Bolesch, D.G., Keasling, J.D., 2000. Polyphosphate binding and chain length recognition of Escherichia coli exopolyphosphatase. J. Biol. Chem. 275, 33814–33819" for more information.
Proper citation: RRID:Addgene_38328 Copy
Species: Escherichia coli
Genetic Insert: beta-glucuronidase
Vector Backbone Description: Backbone Size:2792; Vector Backbone:pBAV1k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: In our experience, plasmid yields are highest when cells are harvested at late log stage.
Proper citation: RRID:Addgene_30501 Copy
Species: Escherichia coli
Genetic Insert: beta-glucuronidase
Vector Backbone Description: Backbone Size:2792; Vector Backbone:pBAV1k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: In our experience, plasmid yields are highest when cells are harvest at late log stage.
Proper citation: RRID:Addgene_30503 Copy
Species: Escherichia coli
Genetic Insert: beta-glucuronidase
Vector Backbone Description: Backbone Size:2792; Vector Backbone:pBAV1k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: In our experience, plasmid yields are highest when cells are harvested at late log stage.
Proper citation: RRID:Addgene_30502 Copy
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