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Species: Caenorhabditis elegans
Genetic Insert: C113S K+ voltage sensitive channel subunit 1.2
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid contains K681I and T208A mutations in KVS-1 compared the reference AAO65852.1 that do not impact function.
Proper citation: RRID:Addgene_113068 Copy
Species: Caenorhabditis elegans
Genetic Insert: MiRP K+ channel accessory Subunit 3
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid contains a T61A mutation compared to the reference NP_490778.1 that does not impact function.
Proper citation: RRID:Addgene_113071 Copy
Species: Caenorhabditis elegans
Genetic Insert: C113S K+ voltage sensitive channel subunit 1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_113070 Copy
Species: Caenorhabditis elegans
Genetic Insert: nud-1
Vector Backbone Description: Backbone Marker:L4440 is available at Addgene; Backbone Size:2790; Vector Backbone:L4440; Vector Types:Worm Expression, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: Full length cDNA for C. elegans open-reading frame F53A2.4, encoding the nud-1 gene, cloned into RNAi feeding vector L4440. Can be used for RNAi feeding; should result in embryonic lethality at high levels of induction.
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
Proper citation: RRID:Addgene_11337 Copy
Species: Caenorhabditis elegans
Genetic Insert: nud-1
Vector Backbone Description: Backbone Marker:see pLexA map in "author's map"; Backbone Size:5700; Vector Backbone:pLexA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Full-length cDNA for C. elegans open-reading frame F53A2.4, encoding the nud-1 gene, cloned into the yeast two-hybrid DNA-binding domain vector pLexA. This vector can be used as the bait for a yeast two-hybrid screen. See "author's map" for picture of empty pLexA vector.
Please note that Addgene's sequencing result differs from GenBank ID NM_067348, which indicates mutations in nud-1 are present at K136R, C220G and E235G.
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
Proper citation: RRID:Addgene_11339 Copy
Species: Caenorhabditis elegans
Genetic Insert: lis-1
Vector Backbone Description: Backbone Marker:see pLexA map in "author's map"; Backbone Size:5700; Vector Backbone:pLexA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Full-length cDNA for C. elegans open-reading frame T03F6.5, encoding the lis-1 gene, cloned into the yeast two-hybrid DNA-binding domain vector pLexA. This vector can be used as the bait for a yeast two-hybrid screen. See "author's map" for picture of empty pLexA vector.
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
Proper citation: RRID:Addgene_11338 Copy
Species: Caenorhabditis elegans
Genetic Insert: GFP-PH
Vector Backbone Description: Vector Backbone:pCFJ151; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_107020 Copy
Species: Caenorhabditis elegans
Genetic Insert: GFP-PH
Vector Backbone Description: Vector Backbone:pCFJ151; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_107016 Copy
Species: Caenorhabditis elegans
Genetic Insert: GFP-PH
Vector Backbone Description: Vector Backbone:pCFJ151; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_107013 Copy
Species: Caenorhabditis elegans
Genetic Insert: GFP-PH
Vector Backbone Description: Vector Backbone:pCFJ151; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_107019 Copy
Species: Caenorhabditis elegans
Genetic Insert: GFP-PH
Vector Backbone Description: Vector Backbone:pCFJ151; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_107022 Copy
Species: Caenorhabditis elegans
Genetic Insert: zif-1
Vector Backbone Description: Backbone Marker:Christian Frøkjær-Jensen (Erik Jorgensen lab); Backbone Size:7328; Vector Backbone:pCFJ151; Vector Types:Targeting vector for Mos1 transposon mediated single copy transgene insertion; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100006 Copy
Species: Caenorhabditis elegans
Genetic Insert: DAF-16a
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:7430; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_132367 Copy
Species: Caenorhabditis elegans
Genetic Insert: INS-6
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:8291; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
References:
Comments: Plasmid contains the cDNA of ins-6 based on wormbase sequence.
Proper citation: RRID:Addgene_132365 Copy
Species: Caenorhabditis elegans
Genetic Insert: mNG-C1^SEC^3xFlag
Vector Backbone Description: Backbone Size:3881; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_132523 Copy
Species: Caenorhabditis elegans
Genetic Insert: Let-60
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_66861 Copy
Species: Caenorhabditis elegans
Genetic Insert: mKate2-C1^SEC^3xFlag
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: THE DEPOSITING LAB NO LONGER USES THIS CONSTRUCT AND RECOMMENDS pDD287 (https://www.addgene.org/70685/). The full sequence for this plasmid can still be found by clicking the link next to "Depositor Sequences".
IMPORTANT NOTE: The repeat regions in this plasmid make it susceptible to recombination. The depositing lab grows the plasmid at 30°C. You may also want to prep and digest multiple single colonies to verify that you have a clone that has not recombined.
Proper citation: RRID:Addgene_66826 Copy
Species: Caenorhabditis elegans
Genetic Insert: TagRFP-T-C1^SEC^3xFlag
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: THE DEPOSITING LAB NO LONGER USES THIS CONSTRUCT AND RECOMMENDS pDD286 (https://www.addgene.org/70684/). The full sequence for this plasmid can still be found by clicking the link next to "Addgene Sequences" and scrolling to the bottom of the page for "Full Addgene NGS sequence".
IMPORTANT NOTE: The repeat regions in this plasmid make it susceptible to recombination. The depositing lab grows the plasmid at 30°C. You may also want to prep and digest multiple single colonies to verify that you have a clone that has not recombined.
Proper citation: RRID:Addgene_66825 Copy
Species: Caenorhabditis elegans
Genetic Insert: CST-1
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene sequencing results find amino acid changes S48N and H50R compared to available CST-1 reference sequences.
Proper citation: RRID:Addgene_66859 Copy
Species: Caenorhabditis elegans
Genetic Insert: YAP-1
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:PD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
References:
Comments: I performed PCR on C.elegans genome to isolate the genome of YAP-1 which contain 5' UTR and the coding exons and introns. I subcloned the product into PstI/SmaI sites of PD95.75, so that GFP is fused to the last exon. Serine 104 was mutated to alanine.
Proper citation: RRID:Addgene_66858 Copy
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