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Species: Other
Genetic Insert: TALE-Intron-N-DddAtox half (G1397N)-T2A-TALE-C-DddAtox half (G1397C)-TadA8e
Vector Backbone Description: Vector Backbone:pYPQ121; Vector Types:Plant Expression, Plastid base editing; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_218171 Copy
Species: Other
Genetic Insert: TALE-Intron-TadAC-DddA full (E1347A)-UGI
Vector Backbone Description: Vector Backbone:pYPQ121; Vector Types:Plant Expression, Plastid base editing; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_218173 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS1-RK2BOM, CV-aadA_nt, CV-ccdB-SwaI.
Vector Backbone Description: Backbone Size:5008; Vector Backbone:S7942NS1-RK2BOM_aadA_nt_ccdB-SwaI; Vector Types:; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_120084 Copy
Species:
Genetic Insert: pBS MCS
Vector Backbone Description: Backbone Size:6194; Vector Backbone:pAM4787; Vector Types:shuttle vector; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_120086 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: Plasmid assembled from 4 CYANO-VECTOR modular devices: CV-pANS, CV-oribom, CV-aadA, PconII-LTRBS-yemGFP.
Vector Backbone Description: Backbone Size:7032; Vector Backbone:pBR322-aadA; Vector Types:other; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_120088 Copy
Species: Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942
Genetic Insert: pAM4787 with pSYSA-TA1 cassette cloned into the SacI restriction site.
Vector Backbone Description: Backbone Size:7774; Vector Backbone:pAM4787; Vector Types:other; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_120089 Copy
Species: Broad range of bacteria
Genetic Insert: Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aadA, CV-PconII-LTRBS-GFPmut2
Vector Backbone Description: Backbone Size:8069; Vector Backbone:RSF1010Y25F_aadA_PconII-LTRBS-GFPmut2; Vector Types:Other; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_120079 Copy
Species: Streptococcus pyogenes
Genetic Insert: tracrRNA-Cas9
Vector Backbone Description: Backbone Marker:Standard European Vector Architecture; Backbone Size:3882; Vector Backbone:pSEVA421; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_138709 Copy
Species: Other
Genetic Insert: mdlC (benzylformate decarboxylase) C -terminal his tag
Vector Backbone Description: Backbone Marker:None; Backbone Size:2100; Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/820787v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_134590 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160172 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160174 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160175 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_160168 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Keunsub Lee; Backbone Size:6149; Vector Backbone:modified pTF505; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_187875 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Keunsub Lee; Backbone Size:6149; Vector Backbone:modified pTF505; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_187874 Copy
Species: Other
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Ephraim Aliu; Backbone Size:6494; Vector Backbone:pEA106; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_187873 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_190158 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Andrew Gleave; Backbone Size:11612; Vector Backbone:pART27; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: pART27 is the binary vector corresponding to pART7. It harbours a transfer DNA gene cassette (tDNA) between the left and right borders to be integrated into the genome of higher plants by Agrobacterium-mediated gene transfer. A single NotI restriction site allows to integrate a NotI-restricted gene cassette from pART7 into the tDNA of pART27. pART27 is a derivative of pMON530 (Rogers et al., 1987; doi: 10.1016/0076-6879(87)53058-0). See the Supplemental Document under Resource Information for more details, including annotated maps.
Proper citation: RRID:Addgene_247986 Copy
Species: Xenorhabdus nematophila ATCC 19061
Genetic Insert: NrdJ-1C - XtpS
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: This plasmid encodes the termination unit of the non-ribosomal peptide synthetase (NRPS) called xenotetrapeptide synthetase (XtpS), split at an XUTI site and fused to the C-terminal part of the split intein NrdJ-1.When co-expressed with pIN086 and pIN200, the three fragments splice into a functional NRPS that produces the non-ribosomal peptide (NRP) called xenotetrapeptide (XTP; cyclo[Leu-D-Val-Val-D-Val]; CC(C)C[C@H]1C(=O)N[C@@H](C(=O)N[C@H](C(=O)N[C@@H](C(=O)N1)C(C)C)C(C)C)C(C)C; 410.6 g/mol).
For successful NRP production, the three plasmids must be co-transformed into an E. coli strain that expresses a broad-spectrum phosphopantetheinyl transferase, such as MtaA (DH10B::mtaA) or Sfp (BAP1), which is required for the post-translational activation of the T domains.
Expression is controlled by an araBAD promoter, inducible with 0.2% arabinose.
Proper citation: RRID:Addgene_255795 Copy
Species: Xenorhabdus nematophila ATCC 19061
Genetic Insert: SspGyrBC - XtpS
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: This plasmid encodes the termination unit of the non-ribosomal peptide synthetase (NRPS) called xenotetrapeptide synthetase (XtpS), split at an XUC site and fused to the C-terminal part of the split intein SspGyrB.When co-expressed with pIN097 and pIN202, the three fragments splice into a functional NRPS that produces the non-ribosomal peptide (NRP) called xenotetrapeptide (XTP; cyclo[Leu-D-Val-Val-D-Val]; CC(C)C[C@H]1C(=O)N[C@@H](C(=O)N[C@H](C(=O)N[C@@H](C(=O)N1)C(C)C)C(C)C)C(C)C; 410.6 g/mol).
For successful NRP production, the three plasmids must be co-transformed into an E. coli strain that expresses a broad-spectrum phosphopantetheinyl transferase, such as MtaA (DH10B::mtaA) or Sfp (BAP1), which is required for the post-translational activation of the T domains.
Expression is controlled by an araBAD promoter, inducible with 0.2% arabinose.
Proper citation: RRID:Addgene_255797 Copy
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