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Species: Other
Genetic Insert: gateway cassette
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3048; Vector Backbone:pGEM-T easy; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Use this vector for 3-way multi-site Gateway reactions with two regions of homology flanking an insert - this final vector can be used as a template for repair (homology-directed repair) after CRISPR double-strand break. This plasmid was constructed by A-tailing the Gateway cassette (R1R2) and blunt/TA Ligase reaction with pGEM-T Easy to create pGEM-gate.
Proper citation: RRID:Addgene_113758 Copy
Species: S. pyogenes, Z. mays
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T easy; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Also has aminoglycosyl phosphotransferase driven by 35S promoter (G418 resistance). We are not sure of the backbone -- it came from a plasmid that was made many many years ago. It aligns well with pGEM-T Easy (which a lot of our plasmids are based on) so if we had to guess, we would say the backbone is pGEM-T Easy.
Proper citation: RRID:Addgene_113743 Copy
Species: S. pyogenes, Z. mays
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T easy; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Also has hygromycinR driven by 35S promoter. We are not sure of the backbone -- it came from a plasmid that was made many many years ago. It aligns well with pGEM-T Easy (which a lot of our plasmids are based on) so if we had to guess, we would say the backbone is pGEM-T Easy.
Proper citation: RRID:Addgene_113742 Copy
Species: S. pyogenes, Z. mays
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T easy; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Also has zeocinR driven by 35S promoter. We are not sure of the backbone -- it came from a plasmid that was made many many years ago. It aligns well with pGEM-T Easy (which a lot of our plasmids are based on) so if we had to guess, we would say the backbone is pGEM-T Easy.
Proper citation: RRID:Addgene_113744 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pFRT-EGFP-DEST; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_106348 Copy
Species:
Genetic Insert: ccdB gene, Chloramphenicol resistance gene
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_114019 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCWX; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: This plasmid allows for 3-plasmid Gateway cloning of a gene of interest with a promoter of choice. GOI is in pENTRL1L2 and promoter is in pENTRL4L1R
Proper citation: RRID:Addgene_114321 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCWX; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: This plasmid allows for 3-plasmid Gateway cloning of a gene of interest with a promoter of choice. GOI is in pENTRL1L2 and promoter is in pENTRL4L1R
Proper citation: RRID:Addgene_114320 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCWX; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: This plasmid allows for 3-plasmid Gateway cloning of a gene of interest with a promoter of choice. GOI is in pENTRL1L2 and promoter is in pENTRL4L1R
Proper citation: RRID:Addgene_114319 Copy
Species:
Genetic Insert: Gateway ccdB reading frame cassette
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP-Y; Vector Types:Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_11479 Copy
Species:
Genetic Insert: Gateway ccdB reading frame cassette
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP-Y; Vector Types:Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_11480 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:8724; Vector Backbone:pDest-590; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_11519 Copy
Species:
Genetic Insert: None
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:8266; Vector Backbone:pDest-560; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_11518 Copy
Species:
Genetic Insert: None
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:8932; Vector Backbone:pDest-560; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_11520 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:8963; Vector Backbone:pZmUBQ:GW; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_132358 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pMH-EF1-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_133018 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pMH-CMV-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_133017 Copy
Species:
Genetic Insert: HIS3 reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pDEST6; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please note that the ccdB cassette in the author's map (click on 'View map') is shown in the reverse orientation.
Y1H Destination vector that can be used in conventional Gateway LR cloning reactions. This vector contains a Gateway cassette with AttR4 and AttL1 recombination sites and a HIS3 reporter gene. For more information and protocols, see Deplancke et al. 2004 Genome Res 14(10B):2093 and Deplancke et al. 2006 CSH Protocols.
Proper citation: RRID:Addgene_13349 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_66936 Copy
Species:
Genetic Insert: traJ, cat, oriT
Vector Backbone Description: Vector Backbone:pKD46; Vector Types:Red Recombinase; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Conjugally transferable red recombinase vector pMJH46
Proper citation: RRID:Addgene_67272 Copy
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