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Species: S. pyogenes
Genetic Insert: tracrRNA
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_46570 Copy
Species: S. pyogenes
Genetic Insert: tracrRNA
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_46569 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:4980; Vector Backbone:pBR322; Vector Types:sequencing vector; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_46858 Copy
Species:
Genetic Insert: esaI-term
Vector Backbone Description: Backbone Size:4511; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: esaI is 636 bp and indicated as lowercase letters in the partial sequence (between BamHI and XhoI sites). See pAC-EsaR for lac promoter and esaR sequence information.
Proper citation: RRID:Addgene_47660 Copy
Species:
Genetic Insert: esaR-I70V/D91G
Vector Backbone Description: Backbone Size:3880; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: esaR-I70V/D91G (750 bp, indicated as lowercase letters in the partial sequence) is located between KpnI and BamHI sites in the same way as wt esaR in pAC-EsaR. Please see pAC-EsaR for lac promoter and terminator sequence information and plasmid map.
Proper citation: RRID:Addgene_47644 Copy
Species:
Genetic Insert: esaR-I70V/D91G/V220A
Vector Backbone Description: Backbone Size:3880; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: esaR-I70V/D91G/V220A (750bp, indicated as lowercase letters in the partial sequence) is located between KpnI and BamHI sites in the same way as wt esaR in pAC-EsaR. Please see pAC-EsaR for lac promoter and terminator sequence information and plasmid map.
Proper citation: RRID:Addgene_47647 Copy
Species:
Genetic Insert: esaR-V220A
Vector Backbone Description: Backbone Size:3880; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: esaR-V220A (750bp, indicated as lowercase letters in the partial sequence) is located between KpnI and BamHI sites in the same way as wt esaR in pAC-EsaR. Please see pAC-EsaR for lac promoter and terminator sequence information and plasmid map.
Proper citation: RRID:Addgene_47645 Copy
Species:
Genetic Insert: esaR-D91G
Vector Backbone Description: Backbone Size:3880; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: esaR-D91G (750bp, indicated as lowercase letters in the partial sequence) is located between KpnI and BamHI sites in pAC-EsaR in the same way as wt esaR in pAC-EsaR. Please see pAC-EsaR for lac promoter and terminator sequence information and plasmid map.
Proper citation: RRID:Addgene_47646 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Dieter Haas; Backbone Size:8270; Vector Backbone:pME6010; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_48107 Copy
Species: M.jannaschii
Genetic Insert: M.j. p-azidohenylalanine RS (2 copies +tRNA)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_31186 Copy
Species:
Genetic Insert: ccdB and Cm resistance gene
Vector Backbone Description: Backbone Size:12617; Vector Backbone:pDD62; Vector Types:; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_31224 Copy
Species: Synechocystis
Genetic Insert: pJT118
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:1839; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_31395 Copy
Species: T7 phage
Genetic Insert: RNA polymerase
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: For expression: E. coli CV2
N823D mutation should not affect plasmid function.
Proper citation: RRID:Addgene_31491 Copy
Species:
Genetic Insert: TetR
Vector Backbone Description: Backbone Marker:Schmidt-Dannert Lab; Backbone Size:3110; Vector Backbone:pACBB; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_32555 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: PpsbAI::luxCDE, PpsbAI::luxAB
Vector Backbone Description: Vector Backbone:pAM1582; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Chloramphenicol
References:
Comments: NSII.1 vector
Lux Genes: luxAB & luxCDE
synthesizes luciferase and substrate in S. elongatus
SmaI--PvuII fragment containing PpsbAI::luxCDE was cloned into SmaI site of pAM1582. Orientation of PpsbAI::luxCDE is same as that of PpsbAI::luxAB. NS2.1, PpsbAI::luxCDE, PpsbAI::luxAB plasmid.
Proper citation: RRID:Addgene_40245 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: S. cerevisiae Hsp82
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC-Duet1 and pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_41187 Copy
Species:
Genetic Insert: flaA-GFP
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pTLP6; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: The ~1kb BamHI/SphI flaAGFP fragment from pflaG into the ~4.6kb BamHI/SphI fragment of the ColE1 replicon pTLP6.
Proper citation: RRID:Addgene_45476 Copy
Species: S. pyogenes
Genetic Insert: dCas9 (bacteria)
Vector Backbone Description: Vector Backbone:p15A vector; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_44249 Copy
Species: Pseudomonas putida
Genetic Insert: AlkS(WT)
Vector Backbone Description: Backbone Size:3997; Vector Backbone:pACYC; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_44382 Copy
Species: Pseudomonas putida
Genetic Insert: AlkSm4-3
Vector Backbone Description: Backbone Size:3984; Vector Backbone:pACYC; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_44383 Copy
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