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Species: Viruses (Polyomaviridae)
Genetic Insert: Full genome of BoPyV2a isolate S22
Vector Backbone Description: Backbone Marker:Christopher Buck lab, Addgene plasmid 24755; Backbone Size:2131; Vector Backbone:pFunnyfarm; Vector Types:Viral clone; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments: Genome can be liberated by digestion with SacII
Proper citation: RRID:Addgene_62356 Copy
Species: Viruses (Polyomaviridae)
Genetic Insert: Full genome of BoPyV3 isolate S22
Vector Backbone Description: Backbone Marker:Christopher Buck lab, Addgene plasmid 24755; Backbone Size:2131; Vector Backbone:pFunnyfarm; Vector Types:Viral clone; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments: Genome can be liberated by digestion with EcoRI
Proper citation: RRID:Addgene_62368 Copy
Species: Viruses (Polyomaviridae)
Genetic Insert: Full genome of BoPyV3 isolate S23
Vector Backbone Description: Backbone Marker:Christopher Buck lab, Addgene plasmid 24755; Backbone Size:2131; Vector Backbone:pFunnyfarm; Vector Types:Viral clone; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments: Genome can be liberated by digestion with EcoRI
Proper citation: RRID:Addgene_62369 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4871; Vector Backbone:pPICZalpha C; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments: C-terminal tags are separated by stop codons. Depending on the LP2 primer used in SLIC, the desired C-terminal tag(s) can be fused to the target gene.
Use the one of the following linearization primers pairs for SLIC cloning. Choose one LP1 forward vector primer and one LP2 reverse vector primer:
LP1 forward vector primer:
SLIC Primer (3C site) 5' GGGCCCCTGGAACAGAACTTCCAG 3'
LP2 - select an LP2 primer below depending on which C-terminal tags you want to include fused to your gene of interest:
none SLIC Primer 5' CGCCATTAACCTGATGTTCTGGGG 3'
10His- SLIC Primer 5`GAGCATCATCATCATCACCAC 3'
StrepOne - SLIC Primer 5`AGCGCTTGGAGCCACCCGCAG 3'
S-Tag - SLIC Primer 5`AAAGAAACCGCTGCTGCTAAATTCG 3'
HPC4 - SLIC Primer 5`GAGGACCAGGTGGACCCCCGG 3'
Æ54CPD - SLIC Primer 5`GGCAGCGGCAAGATCCTGCAC 3'
Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_55190 Copy
Species:
Genetic Insert: None
Vector Backbone Description: Vector Backbone:None; Vector Types:; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments: Derived from Lajoie, et. al., Genomically recoded organisms expand biological functions, Science, 2013, with the following genomic modifications: ΔmutS:zeo, ΔtolC, Δbla:tolC, SerB-/ΔSerB
Proper citation: RRID:Addgene_68306 Copy
Species: Other
Genetic Insert: pOst1-pro-af(MUT2)-E2-Crimson
Vector Backbone Description: Vector Backbone:pPICZalpha; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments: Integration plasmid in the pAOX1 locus.
Proper citation: RRID:Addgene_117663 Copy
Species: Other
Genetic Insert: pre-Ost1-pro-alphaf-Btl2
Vector Backbone Description: Vector Backbone:pPICZalpha; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments: Integration plasmid in the pAOX1 locus.
Proper citation: RRID:Addgene_117665 Copy
Species: Other
Genetic Insert: pre-pro-alphaf(I)-E2-Crimson
Vector Backbone Description: Vector Backbone:pPICZalpha; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments: Integration plasmid in the pAOX1 locus.
Proper citation: RRID:Addgene_117660 Copy
Species: Other
Genetic Insert: pOst1-pro-af(MUT1)-E2-Crimson
Vector Backbone Description: Vector Backbone:pPICZalpha; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments: Integration plasmid in the pAOX1 locus.
Proper citation: RRID:Addgene_117662 Copy
Species: Other
Genetic Insert: pre-Ost1-alphaf(I)-E2-Crimson
Vector Backbone Description: Vector Backbone:pPICZalpha; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments: Integration plasmid in the pAOX1 locus.
Proper citation: RRID:Addgene_117661 Copy
Species: Other
Genetic Insert: pre-Ost1-alphaf(I)-Btl2
Vector Backbone Description: Vector Backbone:pPICZalpha; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments: Integration plasmid in the pAOX1 locus.
Proper citation: RRID:Addgene_117667 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtIRT1
Vector Backbone Description: Backbone Size:3329; Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments: insert amino acid sequence: MASNSALLMKTIFLVLIFVSFAISPATSTAPEECGSESANPCVNKAKALPLKVIAIFVILIASMIGVGAPLFSRNVSFLQPDGNIFTIIKCFASGIILGTGFMHVLPDSFEMLSSICLEENPWHKFPFSGFLAMLSGLITLAIDSMATSLYTSKNAVGIMPHGHGHGHGPANDVTLPIKEDDSSNAQLLRYRVIAMVLELGIIVHSVVIGLSLGATSDTCTIKGLIAALCFHQMFEGMGLGGCILQAEYTNMKKFVMAFFFAVTTPFGIALGIALSTVYQDNSPKALITVGLLNACSAGLLIYMALVDLLAAEFMGPKLQGSIKMQFKCLIAALLGCGGMSIIAKWA
Proper citation: RRID:Addgene_117884 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtZIF1
Vector Backbone Description: Backbone Size:3329; Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments: insert amino acid sequence: MAEEYKEALLEKQNYHDGCPGCKVEQMKQLRRGYPYLELSFVWIIVLSTSLPISSLYPFLYYMIEDFGVAKTEKDIGFYAGFVGCSFMLGRALTSVFWGIVADRYGRKPIILLGTISIAIFNALFGLSSNFWMAIGTRFLLGSFNCLLGTMKAYASEIFRDEYQATAMSAVSTAWGIGLIIGPALGGFLAQPADKYPNVFSQESLFGRFRYALPCFTISAFALLVTVLCCFIPETLHNHKLDSLSHDDSYDILEAASHESSPSTGKAGKNERKASQSLLKNWPLMSSIIVYCVLCLHDTAYSEIFALWANSPRKYGGLSYSTNEVGTVLAISGLGLFSFQVFVYPLAEKLLGPVLVTRYAGALMIPIQMSYPFIAGLSGLSLSLMLNCASILINVLSVSAITGLLILQNRAVDQSQRGAANGIAMTAMSLFKTVGPAGAGILFSWSERRLNAAFLPGSHMVFFVLNVIVVVGVALTFKPFLTTSRR
Proper citation: RRID:Addgene_117885 Copy
Species: Arabidopsis thaliana
Genetic Insert: CHL1T101D-eGFP
Vector Backbone Description: Backbone Size:3329; Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments:
Proper citation: RRID:Addgene_117880 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtALMT1-upGFP 5'
Vector Backbone Description: Backbone Size:3329; Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments:
Proper citation: RRID:Addgene_117887 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtALMT1-upGFP 3'
Vector Backbone Description: Backbone Size:3329; Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments:
Proper citation: RRID:Addgene_117889 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtALMT-uPIC
Vector Backbone Description: Backbone Size:3329; Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments:
Proper citation: RRID:Addgene_117872 Copy
Species: Zea mays
Genetic Insert: NiaP
Vector Backbone Description: Backbone Size:3329; Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments: insert amino acid sequence: MAPEMMEEGQSASYTVDDALLSSGFGRFQILILSYAGIGLIAEAMEMMLLSFVGPSVQLEWKLTSHQESMITSVVFVGMLIGAYSWGVVSDNYGRRRGFLFTAIMTSGAGFFSAFAPNYLSLISLRFLVGIGLGGGPVLGSWFLEFVPAPTRGTWMVVFSAFWTVGTILEASLAWTIMPKFGWRWLLALSAVPSFLLLLFYAITPESPRFLCMKGRTTEAVDILEKMARLNNVQLPSGRLVSDKNIELDEVSGSSESTTLLAGAEESDNLNEDQGSDFGGIKSVGKLLAPKLIRATLLLWMAFFGNAFAYYGIVLLTSELSNGNRICAKEDVESVHSTNASLYKNVFISSFAEIPGSFLSAMIVDRFGRKRSMASMLFTSCVFLLPLVFSRTDILTRISLFGARLCISASFTIVYIYAPEIYPTAVRTTGIGIASSVGRIGGILCPLVAVALVHSCQQTTAILLFELVIFLSGLAVSFFPFETKGCRLNDTDVDMN
Proper citation: RRID:Addgene_117874 Copy
Species: Arabidopsis thaliana
Genetic Insert: CHL1 T101A
Vector Backbone Description: Backbone Size:3329; Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments:
Proper citation: RRID:Addgene_117877 Copy
Species: Arabidopsis thaliana
Genetic Insert: CHL1 T101A-eGFP
Vector Backbone Description: Backbone Size:3329; Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments:
Proper citation: RRID:Addgene_117878 Copy
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