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Species: Candidatus Cloacimonetes bacterium (MAG), MWEE01000013
Genetic Insert: Cas8/Cse1 from MWEE01000013, Cas5-HNH
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37995242
Proper citation: RRID:Addgene_205977 Copy
Species: Arabidopsis thaliana
Genetic Insert: pU6-AarI-scaffold-term
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37682951
Proper citation: RRID:Addgene_207917 Copy
Species: Selenomonas sp. isolate RGIG9219 Water_deer_Rumen (MAG), JAGBLH010000220
Genetic Insert: Cas5 from JAGBLH010000220, Cas8-HNH
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37995242
Proper citation: RRID:Addgene_205975 Copy
Species: Candidatus Cloacimonetes bacterium (MAG), MWEE01000013
Genetic Insert: Cas7 from MWEE01000013, Cas5-HNH
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37995242
Proper citation: RRID:Addgene_205979 Copy
Species: Photinus pyralis
Genetic Insert: Firefly Luciferase
Vector Backbone Description: Backbone Size:6685; Vector Backbone:pRRLSIN.cPPT.MND; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38001268
Proper citation: RRID:Addgene_174721 Copy
Species: Homo sapiens
Genetic Insert: ACTB Homology Arms flanking a Blast-mScarlet Cassette
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39668248
Comments: To be co-transfected with sgRNA plasmid px330-PITCh-ACTB https://www.addgene.org/207748 . Please visit https://www.biorxiv.org/content/10.1101/2023.11.01.565029v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_207753 Copy
Species: Photinus pyralis
Genetic Insert: Firefly Luciferase
Vector Backbone Description: Backbone Size:6685; Vector Backbone:pRRLSIN.cPPT.MND; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38001268
Proper citation: RRID:Addgene_174722 Copy
Species: Homo sapiens
Genetic Insert: RNF4
Vector Backbone Description: Vector Backbone:Derived from Addgene ID# 52962 (Zhang Lab).; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37996419
Comments: For performing BioE3 experiments; can be used for transient expression or packaged into lentiviral particles for stable cell line selection. RNF4wt is excisable using EcoR1-Not1.
Proper citation: RRID:Addgene_208046 Copy
Species: Homo sapiens
Genetic Insert: RNF4
Vector Backbone Description: Vector Backbone:Derived from Addgene ID# 52962 (Zhang Lab).; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37996419
Comments: Control for RNF4 BioE3 experiments; contains mutations in the conserved RING domain. RNF4ca is excisable using EcoR1-Not1.
Proper citation: RRID:Addgene_208047 Copy
Species: Selenomonas sp. isolate RGIG9219 Water_deer_Rumen (MAG), JAGBLH010000220
Genetic Insert: Cas6/Csy4 from JAGBLH010000220, Cas8-HNH
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37995242
Proper citation: RRID:Addgene_205973 Copy
Species: Synthetic
Genetic Insert: BirAopt
Vector Backbone Description: Vector Backbone:Derived from Addgene ID# 52962 (Zhang Lab).; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37996419
Comments: Expresses optimized BirA only (with short C-terminal fusion derived from GSQ linker, RBXN polylinker, and P2A remnant). For performing BioE3 experiments; can be used for transient expression or packaged into lentiviral particles for stable cell line selection.
Proper citation: RRID:Addgene_208048 Copy
Species: Synthetic
Genetic Insert: pUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
Vector Backbone Description: Vector Backbone:pegRNA entry vector (MNW320); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39039307
Comments: Please visit https://doi.org/10.1101/2023.09.12.557440 for bioRxiv preprint.
Proper citation: RRID:Addgene_208977 Copy
Species: Active sludge microbial communities of municipal wastewater-treating anaerobic digesters from various locations
Genetic Insert: Ga0116245_10002768 candidate type VII proteins
Vector Backbone Description: Vector Backbone:pET45b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37995242
Proper citation: RRID:Addgene_205984 Copy
Species: Active sludge microbial communities of municipal wastewater-treating anaerobic digesters from various locations
Genetic Insert: Ga0116245_10002768 candidate type VII proteins
Vector Backbone Description: Vector Backbone:pET45b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37995242
Comments: R197S frameshift mutation in Cas7 ORF will not affect plasmid function.
Proper citation: RRID:Addgene_205983 Copy
Species: Homo sapiens
Genetic Insert: SpdCas9 specific Agrp (mouse) MS2gRNA and MCP-MSN
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37813990
Comments: Stbl3 is also a suitable growth strain.
Proper citation: RRID:Addgene_210709 Copy
Species: Homo sapiens
Genetic Insert: MCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2
Vector Backbone Description: Vector Backbone:lenti(AMP); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37813990
Comments: Stbl3 is also a suitable growth strain.
Proper citation: RRID:Addgene_210704 Copy
Species: Homo sapiens
Genetic Insert: H3C2 Homology Arms flanking a mScarlet Tag
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39668248
Comments: To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 https://www.addgene.org/207780 . Please visit https://www.biorxiv.org/content/10.1101/2023.11.01.565029v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_207781 Copy
Species: Homo sapiens
Genetic Insert: LAMP1 Homology Arms flanking a moxGFP-Puro Cassette
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39668248
Comments: To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787): https://www.addgene.org/207787 . Please visit https://www.biorxiv.org/content/10.1101/2023.11.01.565029v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_207788 Copy
Species: Homo sapiens
Genetic Insert: sgRNA Targeting N-terminus of GOLGA2
Vector Backbone Description: Backbone Marker:Addgene #127875; Backbone Size:8947; Vector Backbone:pX330-BbsI-PITCh; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39668248
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.11.01.565029v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_207791 Copy
Species: bacteriophage MS2
Genetic Insert: MS2 loop containing Cj Cas9 gRNA
Vector Backbone Description: Backbone Marker:Genescript; Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37813990
Comments: Stbl3 is also a suitable growth strain.
Proper citation: RRID:Addgene_210711 Copy
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