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Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc with Y717E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_167959 Copy
Species: Escherichia coli
Genetic Insert: lacI
Vector Backbone Description: Vector Backbone:pJOE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_169243 Copy
Species: Escherichia coli
Genetic Insert: rhaRS
Vector Backbone Description: Vector Backbone:pJOE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please note that the 2bp deletion in rTBC0541 (6796bp) as compared to the depositor's sequence does not affect plasmid function.
Proper citation: RRID:Addgene_169244 Copy
Species: Escherichia coli
Genetic Insert: TurboID with 3XHA tag
Vector Backbone Description: Backbone Marker:Twist Biosciences; Vector Backbone:pTWIST Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.01.433373v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172796 Copy
Species: Escherichia coli
Genetic Insert: TurboID with 3XHA tag
Vector Backbone Description: Backbone Marker:Engler et al. 2014; Vector Backbone:pAGM41258; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.01.433373v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172795 Copy
Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc_K540M with Y717E, Y718E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_173451 Copy
Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc_K540M with Y718E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_173450 Copy
Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc_K540M with Y715E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_173448 Copy
Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc_K540M with Y713E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_173447 Copy
Species: Escherichia coli
Genetic Insert: ClpX
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Depositor confirms K408E compared to NC_000913.3 has no impact on function.
Proper citation: RRID:Addgene_111514 Copy
Species: Escherichia coli
Genetic Insert: bacterial mechanosensitive ion channel of large conductance
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:Mammalian Expression, Adeno-associated viral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_107455 Copy
Species: Escherichia Coli
Genetic Insert: bacterial mechanosensitive ion channel of large conductance
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:Mammalian Expression, Adeno-associated viral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_107454 Copy
Species: Escherichia coli
Genetic Insert: ftsB
Vector Backbone Description: Vector Backbone:pJKR-L-tetR; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2018/05/04/314922 for bioRxiv preprint.
Proper citation: RRID:Addgene_107559 Copy
Species: Escherichia coli
Genetic Insert: ftsA
Vector Backbone Description: Vector Backbone:pJKR-L-tetR; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Please note that an IS4 transposase sequence was found between TetR and AmpR during Addgene's quality control. The depositor noted that this sequence should NOT affect plasmid function.
Please visit https://www.biorxiv.org/content/early/2018/05/04/314922 for bioRxiv preprint.
Proper citation: RRID:Addgene_107558 Copy
Species: Escherichia coli
Genetic Insert: rmtB
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: JoVE antibiotic dereplication paper: Zubyk, H.L., Cox, G., Wright, G.D. Antibiotic Dereplication Using the Antibiotic Resistance Platform. J. Vis. Exp. (152), e60536, doi:10.3791/60536 (2019). (https://www.jove.com/video/60536/antibiotic-dereplication-using-the-antibiotic-resistance-platform)
Proper citation: RRID:Addgene_112880 Copy
Species: Escherichia coli
Genetic Insert: NusG
Vector Backbone Description: Backbone Marker:UC Berkeley MacroLab; Backbone Size:6500; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_113122 Copy
Species: Escherichia coli
Genetic Insert: Rho
Vector Backbone Description: Vector Backbone:pET24b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_113121 Copy
Species: Escherichia coli
Genetic Insert: NusG
Vector Backbone Description: Backbone Marker:UC Berkeley MacroLab; Backbone Size:6500; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_113124 Copy
Species: Escherichia coli
Genetic Insert: FtnA
Vector Backbone Description: Vector Backbone:plasmid PAtagRFP-actin (Verkhusha lab/Addgene); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_108969 Copy
Species: Escherichia coli
Genetic Insert: Acetyl-CoA synthetase (ACS)
Vector Backbone Description: Vector Backbone:pETM11; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Wild Type Gene
Proper citation: RRID:Addgene_108943 Copy
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