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Species: E. coli
Genetic Insert: IraD
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pSKB2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229455 Copy
Species: E. coli
Genetic Insert: IraM
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pSKB2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229454 Copy
Species: E. coli
Genetic Insert: IraD (R67A)
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pSKB2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229457 Copy
Species: E. coli
Genetic Insert: IraD (aa 17-130 only)
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pSKB2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229456 Copy
Species: E. coli
Genetic Insert: IraD (W64R)
Vector Backbone Description: Backbone Size:3787; Vector Backbone:pOKD5; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229461 Copy
Species: E. coli
Genetic Insert: RssB (E229C)
Vector Backbone Description: Backbone Size:3787; Vector Backbone:pOKD5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229477 Copy
Species: E. coli
Genetic Insert: RssB
Vector Backbone Description: Backbone Size:3787; Vector Backbone:pOKD5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229463 Copy
Species: E. coli
Genetic Insert: RssB (D58P)
Vector Backbone Description: Backbone Size:3787; Vector Backbone:pOKD5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229465 Copy
Species: E. coli
Genetic Insert: RssB (Q254A)
Vector Backbone Description: Backbone Size:3787; Vector Backbone:pOKD5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229468 Copy
Species: E. coli
Genetic Insert: RssB (D104A)
Vector Backbone Description: Backbone Size:3787; Vector Backbone:pOKD5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229469 Copy
Species: E. coli
Genetic Insert: RssB (D104N)
Vector Backbone Description: Backbone Size:5387; Vector Backbone:pSY5; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229471 Copy
Species: E. coli
Genetic Insert: RssB (D104A)
Vector Backbone Description: Backbone Size:5387; Vector Backbone:pSY5; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229470 Copy
Species: E. coli
Genetic Insert: RssB (N256A)
Vector Backbone Description: Backbone Size:3787; Vector Backbone:pOKD5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229473 Copy
Species: E. Coli
Genetic Insert: Ptn5_[Tn5]
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_225785 Copy
Species: E. coli
Genetic Insert: tnsABCD
Vector Backbone Description: Vector Backbone:pIND4; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39324814
Comments: Needs to be grown in E. coli BW25141 (pir+ strain with an anti-CRISPR protein on the chromosome)
Please visit https://pubmed.ncbi.nlm.nih.gov/37662258/ for bioRxiv preprint.
Proper citation: RRID:Addgene_220900 Copy
Species: E. coli
Genetic Insert: EcNR1 pUltraG ScW40 CCA trpS::ZeoR trpT::gentR dgalK lambdaRED::galK
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin (50 ug/mL), Gentamycin (10 ug/mL)
Defining Citation: PMID:28192410
Proper citation: RRID:Addgene_218768 Copy
Species: E. coli
Genetic Insert: pUltraG ScW40CCA BL21 trpT::gentR trpS::zeoR
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin (50 ug/mL), Gentamycin (10 ug/mL)
Defining Citation: PMID:34655653
Proper citation: RRID:Addgene_218769 Copy
Species: E. coli
Genetic Insert: EcTyrRS-pAAFRS-9
Vector Backbone Description: Backbone Size:4091; Vector Backbone:pEVOL-tac; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38913984
Comments: EcY-tRNA-TAG
After the plasmids are propagated, they need to be gel purified by agarose gel because the ATM strains contain a plasmid in their genome.
Proper citation: RRID:Addgene_218767 Copy
Species: E. coli
Genetic Insert: EcWRS-h14
Vector Backbone Description: Backbone Size:4166; Vector Backbone:pEvoltac; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28192410
Comments: EcW-tRNA-TGA
After the plasmids are propagated, they need to be gel purified by agarose gel because the ATM strains contain a plasmid in their genome.
Proper citation: RRID:Addgene_218764 Copy
Species: E. coli
Genetic Insert: speG
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38885650
Proper citation: RRID:Addgene_220478 Copy
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