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Species: Homo sapiens
Genetic Insert: 18 bp RFLP (MluI+KspAI)
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology, Human Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28939824
Comments: Combine this donor plasmid with SpCas9 or SpCas9.D10A with Addgene plasmid 41818 gRNA_AAVS1-T2; Mali et al. (named pgRNA.S1 in Chen et al.) for homology-directed AAVS1 targeting.
Proper citation: RRID:Addgene_100290 Copy
Species: Homo sapiens
Genetic Insert: PuroR.T2A.EGFP expression unit
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28939824
Comments: Combine this donor plasmid with SpCas9 or SpCas9.D10A with Addgene plasmid 41818 gRNA_AAVS1-T2; Mali et al. (named pgRNA.S1 in Chen et al. 2017) for inducing homology-directed AAVS1 targeting. This donor plasmid has its targeting module flanked by the target site (TS) for gRNA.S1: 5’-CTGTCCCCTCCACCCCACAGTggggccactagggacaggattggTGACAGA-3’.
Proper citation: RRID:Addgene_100297 Copy
Species: Homo sapiens
Genetic Insert: PuroR.T2A.EGFP expression unit
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28939824
Comments: Combine this donor plasmid with SpCas9 or SpCas9.D10A with Addgene plasmid 41818 gRNA_AAVS1-T2; Mali et al. (named pgRNA.S1 in Chen et al.) for homology-directed AAVS1 targeting.
Proper citation: RRID:Addgene_100296 Copy
Species: Homo sapiens
Genetic Insert: FTL
Vector Backbone Description: Backbone Size:7800; Vector Backbone:PHAGE-P-CMVt-N-HA-GAW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25476789
Proper citation: RRID:Addgene_100153 Copy
Species: Mus musculus
Genetic Insert: Kdm6b
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28723896
Proper citation: RRID:Addgene_100278 Copy
Vector Backbone Description: Vector Backbone:pLKO_TRC005; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28805815
Proper citation: RRID:Addgene_100277 Copy
Genetic Insert: LF82_782
Vector Backbone Description: Backbone Size:4502; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29762554
Proper citation: RRID:Addgene_100385 Copy
Species: Homo sapiens
Genetic Insert: MAT2A
Vector Backbone Description: Backbone Marker:Carson Thoreen at Yale University; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29123071
Proper citation: RRID:Addgene_100521 Copy
Species: X. dendrorhous
Genetic Insert: GGPP synthase
Vector Backbone Description: Backbone Size:4846; Vector Backbone:pLM304; Vector Types:Yeast Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29789561
Comments: Addgene NGS sequencing found the following mutations compared to the provided reference sequence: crtE with M176I, K346R, I347M, E353D mutations, crtI with L107F, H427Q mutations, and crtYB with A190T, E307K mutations. tHMG1 contains only residues 531-1054 compared to the NCBI reference seq [M22002.1]. These mutations/truncation do not affect plasmid function.
Proper citation: RRID:Addgene_100539 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B
Vector Backbone Description: Backbone Size:4885; Vector Backbone:pL1A0*B0*_Leu; Vector Types:Yeast Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29789561
Proper citation: RRID:Addgene_100537 Copy
Species: Rattus norvegicus
Genetic Insert: S6K1
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29123071
Proper citation: RRID:Addgene_100509 Copy
Species: Aedes aegypti, Streptococcus pyogenes
Genetic Insert: AAEL003877-hspcas9-GFP
Vector Backbone Description: Backbone Size:5246; Vector Backbone:PiggyBac; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29138316
Proper citation: RRID:Addgene_100581 Copy
Species: Homo sapiens
Genetic Insert: SESN2
Vector Backbone Description: Vector Backbone:pIC242; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29123071
Proper citation: RRID:Addgene_100519 Copy
Species: Homo sapiens
Genetic Insert: SAMTOR
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29123071
Proper citation: RRID:Addgene_100513 Copy
Species: Homo sapiens
Genetic Insert: Metap2
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29123071
Proper citation: RRID:Addgene_100512 Copy
Vector Backbone Description: Backbone Size:2898; Vector Backbone:pUC-AMPr based; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28988985
Proper citation: RRID:Addgene_100603 Copy
Species: E. coli
Genetic Insert: sgGal4
Vector Backbone Description: Backbone Marker:Addgene #51024; Vector Backbone:pSLQ1651-sgRNA(F+E); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28841410
Proper citation: RRID:Addgene_100549 Copy
Species: Streptococcus pyogenes
Genetic Insert: N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
Vector Backbone Description: Backbone Size:7064; Vector Backbone:pEF1a-FB-puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28841410
Proper citation: RRID:Addgene_100547 Copy
Species: Homo sapiens
Genetic Insert: thymidylate synthetase
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5428; Vector Backbone:pCDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29100421
Proper citation: RRID:Addgene_100545 Copy
Species: Homo sapiens
Genetic Insert: thymidine kinase 1
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5428; Vector Backbone:pCDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29100421
Proper citation: RRID:Addgene_100544 Copy
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