Plasmids   Select Another Resource Report Type

http://www.addgene.org/105868

Species: Homo sapiens
Genetic Insert: Hspa1a (synonym Hsp68) MiniPromoter
Vector Backbone Description: Vector Backbone:unknown; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30765420
Comments: Please see the annotated GenBank file and the additional image file for the specific location of features in this plasmid, including the MiniPromoter.

Proper citation: RRID:Addgene_105868 Copy   

•   Source: Addgene

http://www.addgene.org/105866

Species: Arabidopsis thaliana
Genetic Insert: U6-26p::sgRNA scaffold
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pGEM T-easy; Vector Types:Plant Expression, CRISPR, Synthetic Biology, Subcloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34541122
Comments: The new version of pFH6 (pFH6_new) provides > 10 times higher mutation efficiencies than the original version. In combination with pUB-Cas9, homozygous knockout plants can be created in Arabidopsis in the T2 generation at efficiencies of 50-100%. For cloning procedure, please use the attached protocol which is derived from Hahn et al., 2017 ( http://www.bio-protocol.org/e2384 ).

Proper citation: RRID:Addgene_105866 Copy   

•   Source: Addgene

http://www.addgene.org/105865

Species: Synthetic
Genetic Insert: K-GECO1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29338710
Comments: bioRxiv--A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein (https://doi.org/10.1101/213082)

Proper citation: RRID:Addgene_105865 Copy   

•   Source: Addgene

http://www.addgene.org/105839

Species: E. coli
Genetic Insert: hydroperoxidase II catalase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.

Proper citation: RRID:Addgene_105839 Copy   

•   Source: Addgene

http://www.addgene.org/105796

Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. mCherry descends from the first truly monomeric red fluorescent protein mRFP1, which was obtained by mutating DsRed form Discosoma sp. Note that EGFP and mCherry come from different organisms and their sequences differ greatly. However, GFP-type termini were introduced into mCherry to increase tolerance of N- and C-terminal fusions in mammalian cells (PMID: 15558047). Thus, terminal sequences of EGFP and mCherry are the same.

Proper citation: RRID:Addgene_105796 Copy   

•   Source: Addgene

http://www.addgene.org/105794

Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. mEGFP is an EGFP mutant (A206K) with vastly reduced dimerization. Also called the true monomeric variant A206K (PMID: 11988576, PMID: 22869113).

Proper citation: RRID:Addgene_105794 Copy   

•   Source: Addgene

http://www.addgene.org/105792

Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. Amber is a Venus mutant (Y67C) that neither absorbs nor emits light but retains full tertiary structure of Venus. Used as negative control for FRET measurements. Amber coding sequence was cloned from Addgene ID: 27798.

Proper citation: RRID:Addgene_105792 Copy   

•   Source: Addgene

http://www.addgene.org/105793

Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. Enhanced green florescent protein (EGFP) is a mutant of wild-type GFP with humanized sequence and mutations (F64L, S65T) that improve its spectral properties (PMID: 8805248). EGFP is not truly monomeric in contrast to mEGFP (a property conferred by the A206K mutation, PMID: 11988576, PMID: 22869113).

Proper citation: RRID:Addgene_105793 Copy   

•   Source: Addgene

http://www.addgene.org/105837

Species: Homo sapiens
Genetic Insert: human carbonic anhydrase II thermostable variant
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.

Proper citation: RRID:Addgene_105837 Copy   

•   Source: Addgene

http://www.addgene.org/105798

Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. mCardinal has red-shifted spectra (ex604/em659), which allows for multi-channel imaging and deep-tissue imaging. It was demonstrated that mCardinal and mCherry can be visualized orthogonally without signal cross-talk (PMID: 24633408). mCardinal coding sequence was cloned from Addgene ID: 56172.

Proper citation: RRID:Addgene_105798 Copy   

•   Source: Addgene

http://www.addgene.org/105799

Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. CyOFP1 has large Stokes shift. It is excited in the blue and emits in the orange range. It can be multiplexed with EGFP in fluorescence based assays. Also as acceptor for blue-emitting luciferase (PMID: 27240196). CyOFP1 coding sequence was cloned from Addgene ID 74279.

Proper citation: RRID:Addgene_105799 Copy   

•   Source: Addgene

http://www.addgene.org/105832

Species: Escherichia coli (strain K12)
Genetic Insert: iGluSnFR S72T variant
Vector Backbone Description: Backbone Marker:modified from Invitrogen; Backbone Size:5300; Vector Backbone:pCMV(MinDis) [variant of pDisplay]; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29735711
Comments: pCMV iGluu is the S72T variant of iGluSnFR. (iGluSnFR was created by Marvin et al.2013) bioRxiv: https://doi.org/10.1101/233494

Proper citation: RRID:Addgene_105832 Copy   

•   Source: Addgene

http://www.addgene.org/105840

Species: Homo sapiens
Genetic Insert: Neurogenin 2
Vector Backbone Description: Backbone Marker:custom; Backbone Size:6000; Vector Backbone:pUCM; Vector Types:CRISPR, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29924488
Comments: Please note that this plasmid may runs as a dimer (>21kb). Concatenation/Dimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.

Proper citation: RRID:Addgene_105840 Copy   

•   Source: Addgene

http://www.addgene.org/105849

Species: Mus musculus
Genetic Insert: IgG1 heavy chain
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:4474; Vector Backbone:pFUSEss-CHIg-mG1; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:29875771

Proper citation: RRID:Addgene_105849 Copy   

•   Source: Addgene

http://www.addgene.org/105844

Species: E. coli
Genetic Insert: hydroperoxidase II catalase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.

Proper citation: RRID:Addgene_105844 Copy   

•   Source: Addgene

http://www.addgene.org/105845

Species: E. coli
Genetic Insert: hydroperoxidase II catalase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.

Proper citation: RRID:Addgene_105845 Copy   

•   Source: Addgene

http://www.addgene.org/105842

Species: Homo sapiens
Genetic Insert: Neurogenin 2
Vector Backbone Description: Backbone Marker:custom; Backbone Size:6000; Vector Backbone:pUCM; Vector Types:CRISPR, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29924488

Proper citation: RRID:Addgene_105842 Copy   

•   Source: Addgene

http://www.addgene.org/105843

Species: E. coli
Genetic Insert: hydroperoxidase II catalase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.

Proper citation: RRID:Addgene_105843 Copy   

•   Source: Addgene

http://www.addgene.org/105949

Species: Homo sapiens
Genetic Insert: Plk1
Vector Backbone Description: Vector Backbone:pCS2+ hsp70; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25600237

Proper citation: RRID:Addgene_105949 Copy   

•   Source: Addgene

http://www.addgene.org/105948

Species: Homo sapiens
Genetic Insert: EB3
Vector Backbone Description: Vector Backbone:pCS2+ hsp70; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25600237

Proper citation: RRID:Addgene_105948 Copy   

•   Source: Addgene