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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pEMS1951 Resource Report Resource Website |
RRID:Addgene_105868 | Hspa1a (synonym Hsp68) MiniPromoter | Homo sapiens | Ampicillin | PMID:30765420 | Please see the annotated GenBank file and the additional image file for the specific location of features in this plasmid, including the MiniPromoter. | Vector Backbone:unknown; Vector Types:; Bacterial Resistance:Ampicillin | 2026-09-12 02:14:20 | 0 | |
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pFH6_new Resource Report Resource Website |
RRID:Addgene_105866 | U6-26p::sgRNA scaffold | Arabidopsis thaliana | Ampicillin | PMID:34541122 | The new version of pFH6 (pFH6_new) provides > 10 times higher mutation efficiencies than the original version. In combination with pUB-Cas9, homozygous knockout plants can be created in Arabidopsis in the T2 generation at efficiencies of 50-100%. For cloning procedure, please use the attached protocol which is derived from Hahn et al., 2017 ( http://www.bio-protocol.org/e2384 ). | Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pGEM T-easy; Vector Types:Plant Expression, CRISPR, Synthetic Biology, Subcloning; Bacterial Resistance:Ampicillin | 2026-09-12 02:14:20 | 0 | |
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pcDNA-K-GECO1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_105865 | K-GECO1 | Synthetic | Ampicillin | PMID:29338710 | bioRxiv--A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein (https://doi.org/10.1101/213082) | Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:14:20 | 1 | |
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pBad-HPII Resource Report Resource Website |
RRID:Addgene_105839 | hydroperoxidase II catalase | E. coli | Ampicillin | Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual. | Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:14:20 | 0 | ||
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pKK-TEV-mCherry Resource Report Resource Website |
RRID:Addgene_105796 | Ampicillin | PMID:29590189 | This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. mCherry descends from the first truly monomeric red fluorescent protein mRFP1, which was obtained by mutating DsRed form Discosoma sp. Note that EGFP and mCherry come from different organisms and their sequences differ greatly. However, GFP-type termini were introduced into mCherry to increase tolerance of N- and C-terminal fusions in mammalian cells (PMID: 15558047). Thus, terminal sequences of EGFP and mCherry are the same. | Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin | 2026-09-12 02:14:19 | 0 | |||
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pKK-TEV-mEGFP Resource Report Resource Website |
RRID:Addgene_105794 | Ampicillin | PMID:29590189 | This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. mEGFP is an EGFP mutant (A206K) with vastly reduced dimerization. Also called the true monomeric variant A206K (PMID: 11988576, PMID: 22869113). | Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin | 2026-09-12 02:14:19 | 0 | |||
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pKK-TEV-mAmber Resource Report Resource Website |
RRID:Addgene_105792 | Ampicillin | PMID:29590189 | This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. Amber is a Venus mutant (Y67C) that neither absorbs nor emits light but retains full tertiary structure of Venus. Used as negative control for FRET measurements. Amber coding sequence was cloned from Addgene ID: 27798. | Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin | 2026-09-12 02:14:19 | 0 | |||
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pKK-TEV-EGFP Resource Report Resource Website |
RRID:Addgene_105793 | Ampicillin | PMID:29590189 | This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. Enhanced green florescent protein (EGFP) is a mutant of wild-type GFP with humanized sequence and mutations (F64L, S65T) that improve its spectral properties (PMID: 8805248). EGFP is not truly monomeric in contrast to mEGFP (a property conferred by the A206K mutation, PMID: 11988576, PMID: 22869113). | Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin | 2026-09-12 02:14:19 | 0 | |||
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pBad-CA TAG186 Resource Report Resource Website |
RRID:Addgene_105837 | human carbonic anhydrase II thermostable variant | Homo sapiens | Ampicillin | Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual. | Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | TAG stop codon at site 186 | 2026-09-12 02:14:20 | 0 | |
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pKK-TEV-mCardinal Resource Report Resource Website |
RRID:Addgene_105798 | Ampicillin | PMID:29590189 | This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. mCardinal has red-shifted spectra (ex604/em659), which allows for multi-channel imaging and deep-tissue imaging. It was demonstrated that mCardinal and mCherry can be visualized orthogonally without signal cross-talk (PMID: 24633408). mCardinal coding sequence was cloned from Addgene ID: 56172. | Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin | 2026-09-12 02:14:20 | 0 | |||
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pKK-TEV-CyOFP1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_105799 | Ampicillin | PMID:29590189 | This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. CyOFP1 has large Stokes shift. It is excited in the blue and emits in the orange range. It can be multiplexed with EGFP in fluorescence based assays. Also as acceptor for blue-emitting luciferase (PMID: 27240196). CyOFP1 coding sequence was cloned from Addgene ID 74279. | Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin | 2026-09-12 02:14:20 | 1 | |||
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pCMV iGluu Resource Report Resource Website |
RRID:Addgene_105832 | iGluSnFR S72T variant | Escherichia coli (strain K12) | Ampicillin | PMID:29735711 | pCMV iGluu is the S72T variant of iGluSnFR. (iGluSnFR was created by Marvin et al.2013) bioRxiv: https://doi.org/10.1101/233494 | Backbone Marker:modified from Invitrogen; Backbone Size:5300; Vector Backbone:pCMV(MinDis) [variant of pDisplay]; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Changed Ser 72 to Thr | 2026-09-12 02:14:20 | 0 |
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pUCM-AAVS1-TO-hNGN2 Resource Report Resource Website 10+ mentions |
RRID:Addgene_105840 | Neurogenin 2 | Homo sapiens | Ampicillin | PMID:29924488 | Please note that this plasmid may runs as a dimer (>21kb). Concatenation/Dimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid. | Backbone Marker:custom; Backbone Size:6000; Vector Backbone:pUCM; Vector Types:CRISPR, TALEN; Bacterial Resistance:Ampicillin | 2026-09-12 02:14:20 | 15 | |
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pFUSEss-CHIg-mG1_Arg322Lys Resource Report Resource Website |
RRID:Addgene_105849 | IgG1 heavy chain | Mus musculus | Bleocin (Zeocin) | PMID:29875771 | Backbone Marker:Invivogen; Backbone Size:4474; Vector Backbone:pFUSEss-CHIg-mG1; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin) | Arg322Lys in constant region of IgG1 heavy chain | 2026-09-12 02:14:20 | 0 | |
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pBad-HPII TAG348 Resource Report Resource Website |
RRID:Addgene_105844 | hydroperoxidase II catalase | E. coli | Ampicillin | Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual. | Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | TAG stop codon at site 348 | 2026-09-12 02:14:20 | 0 | |
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pBad-HPII TAG568 Resource Report Resource Website |
RRID:Addgene_105845 | hydroperoxidase II catalase | E. coli | Ampicillin | Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual. | Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | TAG stop codon at site 568 | 2026-09-12 02:14:20 | 0 | |
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CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL)) Resource Report Resource Website 1+ mentions |
RRID:Addgene_105842 | Neurogenin 2 | Homo sapiens | Ampicillin | PMID:29924488 | Backbone Marker:custom; Backbone Size:6000; Vector Backbone:pUCM; Vector Types:CRISPR, TALEN; Bacterial Resistance:Ampicillin | 2026-09-12 02:14:20 | 1 | ||
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pBad-HPII TAG283 Resource Report Resource Website |
RRID:Addgene_105843 | hydroperoxidase II catalase | E. coli | Ampicillin | Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual. | Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | TAG stop codon at site 283 | 2026-09-12 02:14:20 | 0 | |
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HS-DN-Plk1 Resource Report Resource Website |
RRID:Addgene_105949 | Plk1 | Homo sapiens | Ampicillin | PMID:25600237 | Vector Backbone:pCS2+ hsp70; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Dominant negative K82 R | 2026-09-12 02:14:21 | 0 | |
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HS-EB3-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_105948 | EB3 | Homo sapiens | Kanamycin | PMID:25600237 | Vector Backbone:pCS2+ hsp70; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-09-12 02:14:21 | 1 |
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