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http://www.addgene.org/105684

Species: Synthetic
Genetic Insert: RFP Golden Gate dropout
Vector Backbone Description: Backbone Marker:Deuber Lab; Backbone Size:1661; Vector Backbone:YTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29486239
Comments: Dropout flanked by BsaI sites to allow Deuber Lab MoClo 234 parts to be cloned in using Golden Gate assembly.

Proper citation: RRID:Addgene_105684 Copy   

•   Source: Addgene

http://www.addgene.org/105728

Species: Homo sapiens
Genetic Insert: alpha-synuclein E46K
Vector Backbone Description: Backbone Size:2438; Vector Backbone:pT7-7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25657004

Proper citation: RRID:Addgene_105728 Copy   

•   Source: Addgene

http://www.addgene.org/105688

Species: Synthetic
Genetic Insert: P2A-3xFLAG-VHPbeta
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28065601

Proper citation: RRID:Addgene_105688 Copy   

•   Source: Addgene

http://www.addgene.org/105689

Species: Synthetic
Genetic Insert: P2A-3xFLAG-VHPbeta
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28065601

Proper citation: RRID:Addgene_105689 Copy   

•   Source: Addgene

http://www.addgene.org/105936

Species: Homo sapiens
Genetic Insert: AXL
Vector Backbone Description: Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_105936 Copy   

•   Source: Addgene

http://www.addgene.org/105937

Species: Homo sapiens
Genetic Insert: PCNA
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30120238

Proper citation: RRID:Addgene_105937 Copy   

•   Source: Addgene

http://www.addgene.org/105814

Vector Backbone Description: Backbone Marker:Sammarco & Grabczyk (PMID: 16212928); Vector Backbone:BI-16 (constructed based on pcDNA5/FRT/TO from Invitrogen); Vector Types:Mammalian Expression, RNAi, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK-RNAi series which belongs to the pKK vector family. It enables concomitant expression of two genes: 1) a cassette encoding miRNAs that target the gene of interest, and 2) an allele of the gene of interest with the protein coding sequence harbouring silent mutations that make the mRNA insensitive to the miRNAs. As a result, the endogenous alleles of the gene of interest are silenced, whereas the exogenous copy is expressed. Furthermore, the miRNAs are cotranscriptionally expressed with a fluorescent protein reporter so that analysis can be narrowed down to cells that express miRNA, provided that applied assay can distinguish cells expressing reporter. The reporter of miRNA expression has 3 repeats of the nuclear localisation signal of SV40 large T antigen, thus, it localizes to nucleus. This vector is a derivate of the BI16 plasmid (PMID: 16212928) and is suitable for stable cell line generation using the Flp-In system. Transcription of cloned DNA is driven by a tetracycline responsive bidirectional CMV promoter (see Szczesny RJ et al. for detailed description and protocols; bioRxiv, doi: https://doi.org/10.1101/160101).

Proper citation: RRID:Addgene_105814 Copy   

•   Source: Addgene

http://www.addgene.org/105932

Species: Homo sapiens
Genetic Insert: AXL
Vector Backbone Description: Vector Backbone:pcDNA4/TO/myc/HIS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23629654

Proper citation: RRID:Addgene_105932 Copy   

•   Source: Addgene

http://www.addgene.org/105779

Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. mCherry descends from the first truly monomeric red fluorescent protein mRFP1, which was obtained by mutating DsRed form Discosoma sp. Note that EGFP and mCherry come from different organisms and their sequences differ greatly. However, GFP-type termini were introduced into mCherry to increase tolerance of N- and C-terminal fusions in mammalian cells (PMID: 15558047). Thus, terminal sequences of EGFP and mCherry are the same.

Proper citation: RRID:Addgene_105779 Copy   

•   Source: Addgene

http://www.addgene.org/105785

Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method.

Proper citation: RRID:Addgene_105785 Copy   

•   Source: Addgene

http://www.addgene.org/105783

Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. FLAG is a short tag, less likely to interfere with protein function. FLAG tag present at the N-terminus of a protein is cleavable by enterokinase (PMID: 11694294). This is not the case if FLAG is present at the C-terminus of a protein.

Proper citation: RRID:Addgene_105783 Copy   

•   Source: Addgene

http://www.addgene.org/105784

Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. 3xFLAG epitope improves detection by antibody staining in immunocytochemistry and western blot in comparison to FLAG tag. It was also found to be superior to FLAG tag in pull down experiments (PMID: 10769759, 11721627, 22668517). Note that 3xFLAG has different sequence than 3 repeats of FLAG tag.

Proper citation: RRID:Addgene_105784 Copy   

•   Source: Addgene

http://www.addgene.org/105781

Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. mCardinal has red-shifted spectra (ex604/em659), which allows for multi-channel imaging and deep-tissue imaging. It was demonstrated that mCardinal and mCherry can be visualized orthogonally without signal cross-talk (PMID: 24633408). mCardinal coding sequence was cloned from Addgene ID: 56172.

Proper citation: RRID:Addgene_105781 Copy   

•   Source: Addgene

http://www.addgene.org/105780

Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. mRuby3 is brighter than mCherry and has shifted excitation and emission spectra. The mClover3-mRuby3 pair is superior to EGFP-mCherry in FRET analysis. At the time of publication (PMID: 26879144) mRuby3 was the brightest coral-derived RFP in mammalian cells. mRuby3 coding sequence was cloned from Addgene ID: 74252.

Proper citation: RRID:Addgene_105780 Copy   

•   Source: Addgene

http://www.addgene.org/105824

Genetic Insert: mV-TtCBD
Vector Backbone Description: Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28319307

Proper citation: RRID:Addgene_105824 Copy   

•   Source: Addgene

http://www.addgene.org/105825

Genetic Insert: OptoOFF-MxCBD-mFGFR1
Vector Backbone Description: Vector Backbone:pcDNA3.1(-)_CMVtrunc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28319307

Proper citation: RRID:Addgene_105825 Copy   

•   Source: Addgene

http://www.addgene.org/105822

Species: Mycobacterium tuberculosis
Genetic Insert: icl1
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30108733

Proper citation: RRID:Addgene_105822 Copy   

•   Source: Addgene

http://www.addgene.org/105787

Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method.

Proper citation: RRID:Addgene_105787 Copy   

•   Source: Addgene

http://www.addgene.org/105788

Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. Protein A of Staphylococcus aureus binds to IgG of different origin which can be used for affinity purification or antibody-based detection. Protein A (i.e. two IgG-binding domains of protein A) is often used for tandem affinity purification, in which a protein of interest is expressed in fusion with two different tags (for example Protein A-calmodulin binding peptide) (PMID: 3507693, 10504710). In this vector only Protein A is encoded.

Proper citation: RRID:Addgene_105788 Copy   

•   Source: Addgene

http://www.addgene.org/105821

Species: Mus musculus
Genetic Insert: MIRNA301
Vector Backbone Description: Vector Backbone:pSP65; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The insert corresponds to MIRNA301A precursor

Proper citation: RRID:Addgene_105821 Copy   

•   Source: Addgene