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Species: Other
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Penn Vector Core number p2676
Proper citation: RRID:Addgene_105553 Copy
Species: Other
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Penn Vector Core number p2396
Proper citation: RRID:Addgene_105558 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Penn Vector Core number p1845
Proper citation: RRID:Addgene_105556 Copy
Species: Mus musculus
Genetic Insert: Full length MYB (mouse)
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316427
Proper citation: RRID:Addgene_105609 Copy
Species: Homo sapiens
Genetic Insert: shRNA targeting MLL part of MLL-AF9 fusion
Vector Backbone Description: Vector Backbone:LEP (MSCV-LTR-mirE-shRNA-PGK-puro-IRES-GFP); Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316427
Proper citation: RRID:Addgene_105565 Copy
Species: Homo sapiens
Genetic Insert: shRNA targeting MLL part of MLL-AF9 fusion
Vector Backbone Description: Vector Backbone:LEP (MSCV-LTR-mirE-shRNA-PGK-puro-IRES-GFP); Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316427
Proper citation: RRID:Addgene_105566 Copy
Species: Mus musculus
Genetic Insert: TAF4 shRNA
Vector Backbone Description: Vector Backbone:LEP (MSCV-LTR-mirE-shRNA-PGK-puro-IRES-GFP); Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316427
Proper citation: RRID:Addgene_105563 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pUA66; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29149596
Proper citation: RRID:Addgene_105606 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pUA66; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29149596
Proper citation: RRID:Addgene_105604 Copy
Species: S.aureus
Genetic Insert: S.aureus Sortase A, 7+ mutant
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5000; Vector Backbone:pET30b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29200433
Proper citation: RRID:Addgene_105602 Copy
Species: S.aureus
Genetic Insert: S.aureus Sortase A, 7D mutant
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5000; Vector Backbone:pET30b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29200433
Proper citation: RRID:Addgene_105600 Copy
Species: Mus musculus
Genetic Insert: TADA1 shRNA
Vector Backbone Description: Vector Backbone:LEP (MSCV-LTR-mirE-shRNA-PGK-puro-IRES-GFP); Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316427
Proper citation: RRID:Addgene_105568 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. mVenus is a yellow fluorescent protein; acceptor in the commonly used Cerulean-Venus FRET pair. mVenus coding sequence was cloned from Addgene ID: 27793.
Proper citation: RRID:Addgene_105774 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. Amber is a Venus mutant (Y67C) that neither absorbs nor emits light but retains full tertiary structure of Venus. Used as negative control for FRET measurements. Amber coding sequence was cloned from Addgene ID: 27798.
Proper citation: RRID:Addgene_105775 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. mTagBFP coding sequence was cloned from Addgene ID: 62383.
Proper citation: RRID:Addgene_105772 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. mCerulean is a cyan fluorescent protein; donor in the commonly used Cerulean-Venus FRET pair. mCerulean coding sequence was cloned from Addgene ID: 27795.
Proper citation: RRID:Addgene_105773 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. The presence of FLAG facilitates detection of protein of interest by western blot or immunofluorescence.
Note: This plasmid contains a S84C mutation in HygR. The depositor has confirmed that this does not affect plasmid function.
Proper citation: RRID:Addgene_105770 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method.
Proper citation: RRID:Addgene_105771 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. mEGFP is an EGFP mutant (A206K) with vastly reduced dimerization. Also called the true monomeric variant A206K (PMID: 11988576, PMID: 22869113).
Proper citation: RRID:Addgene_105777 Copy
Species: Homo sapiens
Genetic Insert: Metallothionein 1k
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pTyb21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_105707 Copy
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