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Species: Homo sapiens
Genetic Insert: PTEN-del(1-25)
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SIN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21536651
Proper citation: RRID:Addgene_30390 Copy
Species: Homo sapiens
Genetic Insert: GFP-PTEN-WT
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SIN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21536651
Proper citation: RRID:Addgene_30391 Copy
Genetic Insert: F1 TGG, F3 GGG
Vector Backbone Description: Backbone Size:2877; Vector Backbone:pCS2 (modified); Vector Types:mRNA production; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21937602
Proper citation: RRID:Addgene_30710 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: oChIEF-tdtomato
Vector Backbone Description: Backbone Marker:Addgene plasmid 18917; Backbone Size:5079; Vector Backbone:AAV-FLEX-rev-ChR2-tdtomato; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21878344
Comments: Please note this plasmid has a I170V in channelopsin 1. The changed amino acid improves the protein’s function.
To minimize recombination, propagate this plasmids in Stbl2 cells from Invitrogen. Also, to minimize recombination, cells should be cultured at 30 C.
Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended.
For more information on growth conditions, please see www.addgene.org/18917 as this is the backbone vector. Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with PvuII, Sma1, and SnaB1 should be performed. The expected patterns can be calculated from the attached sequence. Please see Reviews in the right column for an image of Addgene's digest with these enzymes.
Proper citation: RRID:Addgene_30541 Copy
Species: Homo sapiens
Genetic Insert: PTEN-del(1-15)
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SIN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21536651
Proper citation: RRID:Addgene_30389 Copy
Species: Homo sapiens
Genetic Insert: GFP-PTEN-Y16C
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SIN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21536651
Proper citation: RRID:Addgene_30542 Copy
Genetic Insert: F1 GTC
Vector Backbone Description: Backbone Size:2877; Vector Backbone:pBlueScript II SK(+); Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21937602
Proper citation: RRID:Addgene_30706 Copy
Genetic Insert: F1 TAG
Vector Backbone Description: Backbone Size:2877; Vector Backbone:pBlueScript II SK(+); Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21937602
Proper citation: RRID:Addgene_30709 Copy
Species: Homo sapiens
Genetic Insert: GFP-PTEN-G129E
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SIN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21536651
Proper citation: RRID:Addgene_30544 Copy
Species: Xenopus laevis
Genetic Insert: lhx1 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21281489
Comments: HNF1 binding site in lhx1 promoter: TTAATGGTTTAC
Proper citation: RRID:Addgene_30492 Copy
Species: Homo sapiens
Genetic Insert: Ahi1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pDsRed2-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19718039
Comments: Total plasmid size: 8.4kb
Proper citation: RRID:Addgene_30495 Copy
Species: synthetic
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCSGFP2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19549299
Comments: Please note that there are some discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor, including a H415R mutation in the downstream dimer component of tdTomato. This mutation may impact tdTomato expression.
Proper citation: RRID:Addgene_30530 Copy
Species: Homo sapiens
Genetic Insert: Barkor
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:4300; Vector Backbone:phrGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21518905
Proper citation: RRID:Addgene_30496 Copy
Species: Homo sapiens
Genetic Insert: Barkor
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:4300; Vector Backbone:phrGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21518905
Proper citation: RRID:Addgene_30499 Copy
Species: Homo sapiens
Genetic Insert: Beclin, Barkor
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:4300; Vector Backbone:phrGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21518905
Proper citation: RRID:Addgene_30498 Copy
Species: Homo sapiens
Genetic Insert: OSR1
Vector Backbone Description: Backbone Size:4352; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21281489
Proper citation: RRID:Addgene_30491 Copy
Species: Homo sapiens
Genetic Insert: p300
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCMVb; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_30490 Copy
Species: Homo sapiens
Genetic Insert: BCL2-associated X protein
Vector Backbone Description: Backbone Size:4700; Vector Backbone:C3-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21458670
Proper citation: RRID:Addgene_30533 Copy
Species: Bos taurus
Genetic Insert: Mature bovine mitochondrial elongation factor Tu
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET24c(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8547323
Proper citation: RRID:Addgene_30535 Copy
Species: Homo sapiens
Genetic Insert: HDAC6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14675537
Proper citation: RRID:Addgene_30482 Copy
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