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Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp11
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: YP_009725312.1
Proper citation: RRID:Addgene_153884 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: fragment SARS-CoV-2 orf1a stop to orf1b stop
Vector Backbone Description: Backbone Size:1968; Vector Backbone:pG9m-2 backbone; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid contains a SARS-CoV-2 viral sequence fragment that covers basepairs 8555 to the end of the genome. Because of sequence complexities in nsp3, it is difficult to synthesize the complete nsp3 gene. This construct may be useful to produce RNA controls for assays, as well as serve as templates to make antigens.
Proper citation: RRID:Addgene_153883 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF6 protein
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740572
Proper citation: RRID:Addgene_153899 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 SARS-CoV-2 E (envelope)
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740570
Proper citation: RRID:Addgene_153898 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF3a protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: YP_009724391.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_153892 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S (surface glycoprotein)
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740568. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_153890 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 M (membrane)
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: YP_009724393.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_153894 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp11
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740578
Proper citation: RRID:Addgene_153823 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF6 protein
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740572
Proper citation: RRID:Addgene_153820 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF10 protein
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740576
Proper citation: RRID:Addgene_153834 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp7
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740578
Proper citation: RRID:Addgene_153844 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp11
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740578
Proper citation: RRID:Addgene_153843 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF10 protein
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740576
Proper citation: RRID:Addgene_153849 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp11
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740578
Proper citation: RRID:Addgene_153841 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 N (nucleocapsid)
Vector Backbone Description: Vector Backbone:EF1-alpha-promoter; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_153989 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: nsp2
Vector Backbone Description: Vector Backbone:EF1-alpha-promoter; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_153988 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: nsp5
Vector Backbone Description: Vector Backbone:EF1-alpha-promoter; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_153987 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: nsp9
Vector Backbone Description: Vector Backbone:EF1-alpha-promoter; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_153986 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: orf9b
Vector Backbone Description: Vector Backbone:EF1-alpha-promoter; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_153981 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: nsp15
Vector Backbone Description: Vector Backbone:EF1-alpha-promoter; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_153985 Copy
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