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Species: Saccharomyces cerevisiae
Genetic Insert: 3' homology for integration site XI-1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176167 Copy
Species: E. coli E2348/69 O127:H6
Genetic Insert: gfcD
Vector Backbone Description: Backbone Marker:from Dr Frank Collart; Vector Backbone:pBH31; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: encodes (pelC signal sequence)-His10-GfcD(22-698)
Proper citation: RRID:Addgene_176200 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 3' homology for integration site XI-2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176169 Copy
Species: Homo sapiens
Genetic Insert: mCherry-INBox-eDHFR
Vector Backbone Description: Vector Backbone:pEM791; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176202 Copy
Species: Mus musculus
Genetic Insert: delFasL
Vector Backbone Description: Backbone Size:0; Vector Backbone:EF.CMV.GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: The CMV promoter was inserted immediately upstream of GFP in the EF.GFP LV to make the intermediate LV, EF.V-CMV.GFP, with a unique EcoRV site available to insert a gene of interest under the control of the EF promoter. Noncleavable mouse delFasL cDNA was constructed using PCR cloning to delete the exon 2 region, which contains the metalloproteinase cleavage site. The delFasL gene was then cloned into the EcoRV site of EF.CMV.GFP to make the EF.delFasL-CMV.GFP LV.
Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.
Proper citation: RRID:Addgene_17620 Copy
Species: Homo sapiens
Genetic Insert: MCAK-mCherry-eDHFR
Vector Backbone Description: Vector Backbone:pEM791; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176204 Copy
Species: Homo sapiens
Genetic Insert: ICN1
Vector Backbone Description: Backbone Marker:Linzhao Cheng Lab; Backbone Size:8200; Vector Backbone:EF.v-CMV.GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Addgene sequence shows that there is an A1902S mutation in Notch1 relative to NCBI sequences. This mutation is not known to affect function.
The CMV promoter was inserted immediately upstream of GFP in the EF.GFP LV to make the intermediate LV, EF.V-CMV.GFP. hICN1 was cloned downstream of the EF-1a promoter.
Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.
Proper citation: RRID:Addgene_17623 Copy
Species: Homo sapiens
Genetic Insert: ICN1
Vector Backbone Description: Backbone Size:11500; Vector Backbone:cFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Addgene sequence shows that there is an A1902S mutation in Notch1 relative to NCBI sequences. This mutation is not known to affect function.
hICN1 driven by the EF-1a promoter and a GFP reporter driven by the ubiquitin C (Ubc) promoter.
Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.
Proper citation: RRID:Addgene_17626 Copy
Species:
Genetic Insert: UBC promoter - GFP - PGK promoter - Hygro
Vector Backbone Description: Backbone Size:0; Vector Backbone:Duet011; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Lentiviral dual promoter/dual-gene expression vector is dubbed Duet (for Dual-gene, excisable transgene).
Proper citation: RRID:Addgene_17627 Copy
Species: Francisella novicida
Genetic Insert: humanized fnCas12a
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR, Luciferase, Synthetic Biology, Expression in microalgae Nannochloropsis oceanica; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176239 Copy
Species: E. coli E2348/69 O127:H6
Genetic Insert: gfcD
Vector Backbone Description: Backbone Marker:from Dr Frank Collart; Vector Backbone:pBH31; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: encodes (pelC signal sequence)-His6-GfcD(22-698)
Proper citation: RRID:Addgene_176199 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:Addgene# 136269; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176234 Copy
Species: Homo sapiens
Genetic Insert: spCas9 and polymerase exo domain fusion
Vector Backbone Description: Vector Backbone:pspCas9-3'UTR-Tetra-COM-Vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176236 Copy
Species: Homo sapiens
Genetic Insert: spCas9 and polymerase exo domain fusion
Vector Backbone Description: Vector Backbone:pspCas9-3'UTR-Tetra-COM-Vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176237 Copy
Species: E. coli E2348/69 O127:H6
Genetic Insert: gfcBCD
Vector Backbone Description: Backbone Marker:from Dr Shoshi Altuvia; Vector Backbone:pSA10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert is a contiguous set of genes gfcB, gfcC, gfcD. All 3 proteins are expressed with their native signal sequences
Proper citation: RRID:Addgene_176193 Copy
Species: E. coli E2348/69 O127:H6
Genetic Insert: gfcBCD
Vector Backbone Description: Backbone Marker:from Dr Shoshi Altuvia; Vector Backbone:pSA10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert is a contiguous set of genes gfcB, gfcC, gfcD. All 3 proteins are expressed with their native signal sequences. Derived from plasmid pSA10(gfcBCD) [Addgene #176193] by mutagenesis to include a 6xHis tag on the C-term of GfcB.
Proper citation: RRID:Addgene_176194 Copy
Species: E. coli E2348/69 O127:H6
Genetic Insert: gfcC
Vector Backbone Description: Vector Backbone:pMCSG7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: encodes His6-(x)8-TeV-GfcC(17-214)
Proper citation: RRID:Addgene_176197 Copy
Species: E. coli E2348/69 O127:H6
Genetic Insert: gfcB
Vector Backbone Description: Vector Backbone:pMCSG7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: encodes His6-(x)8-TeV-GfcB(17-214)
Proper citation: RRID:Addgene_176196 Copy
Species:
Genetic Insert: cI of phage lambda, hok of the R1 plasmid, strA
Vector Backbone Description: Vector Backbone:pMW118; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_176228 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TPK2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015).
Proper citation: RRID:Addgene_176180 Copy
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