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Species:
Genetic Insert: Gateway Destination Vector
Vector Backbone Description: Backbone Size:6465; Vector Backbone:PB-CA; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20960 Copy
Species: Mus musculus
Genetic Insert: HOXA9
Vector Backbone Description: Backbone Size:6500; Vector Backbone:MSCV-PGK-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Addgene has detected several mutations in the UTR region of this construct.
Proper citation: RRID:Addgene_20977 Copy
Species: Mus musculus
Genetic Insert: HOXA9
Vector Backbone Description: Vector Backbone:MSCV (murine stem cell virus); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: *Please note that the full sequence verified by Addgene differs from the original depositor's map and thus the numbers and nomenclature used by the depositor may differ from the Addgene map.*
Depositor's Comments:
We discovered that, although a 1,975-nt full-length Hoxa9 clone (numbered from the protein start site; I.M.A.G.E. clone BC055059) would produce protein when transiently expressed from the murine stem cell virus (MSCV) long terminal repeat, following retroviral transduction, the HOXA9 protein could not be detected. Since this cDNA contained two putative polyadenylation signals in the 3' UTR, we removed these sites to determine if they were interfering with retrovirally mediated protein expression. The site at nt 1798 was replaced using a primer containing a KpnI sequence in place of the AATAAA sequence together with a primer containing the BglII site at nt 749 to amplify a 1,052-nt BglII-KpnI fragment by PCR. A second primer containing the KpnI site in place of the polyadenylation signal at nt 1798 was used in conjunction with a primer containing an AAT-to-CGG change in the polyadenylation signal at nt 1975 and an adjacent XhoI site for cloning to generate a 150-nt KpnI-XhoI fragment. Standard methods were used to combine these fragments to create a 1,975-nt Hoxa9 cDNA encoding an N-terminal FLAG-tagged version of the mature protein but lacking the two putative polyadenylation signals. The resulting MSCV clone produced high levels of FLAG-HOXA9 protein and immortalized BM cells.
Proper citation: RRID:Addgene_20975 Copy
Species: Rattus norvegicus
Genetic Insert: Epsin1 deltaUIM
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20973 Copy
Species: Homo sapiens
Genetic Insert: Histone cluster 1, H2bb
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The accession number for H2B.1 sequence X57127 was obtained from Fraser et al., 2005 (https://doi.org/10.1002/gene.20139). The actual DNA was PCR amplified from HEK293 genomic DNA then cloned into pEGFP-N1 as described in Kanda et al., 1998 (https://doi.org/10.1016/s0960-9822(98)70156-3). That H2B.1-EGFP construct was used as template to synthesize the construct here. An EcoRI site in the H2B.1 sequence was silently mutated. The Venus cDNA was obtained from Dr. Anna-Katerina Hadjantonakis.
Proper citation: RRID:Addgene_20971 Copy
Species: Homo sapiens
Genetic Insert: Histone cluster 1, H2bb
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The accession number for H2B.1 sequence X57127 was obtained from Fraser et al., 2005 (https://doi.org/10.1002/gene.20139). The actual DNA was PCR amplified from HEK293 genomic DNA then cloned into pEGFP-N1 as described in Kanda et al., 1998 (https://doi.org/10.1016/s0960-9822(98)70156-3). That H2B.1-EGFP construct was used as template to synthesize the construct here. An EcoRI site in the H2B.1 sequence was silently mutated. The Strawberry cDNA was obtained from Dr. Roger Tsien.
Proper citation: RRID:Addgene_20970 Copy
Species: C. reinhardtii
Genetic Insert: channelrhodopsin-2
Vector Backbone Description: Backbone Size:9250; Vector Backbone:pLenti-CaMKIIa; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Please see http://www.optogenetics.org
for additional information.
H134R mutation present in the ChR2 sequence is a gain-of-function mutant that produces larger stationary photocurrents in comparison to the wild-type ChR2 sequence
Proper citation: RRID:Addgene_20944 Copy
Species: C. reinhardtii
Genetic Insert: channelrhodopsin-2
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pLenti-EF1a; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Please see http://www.optogenetics.org
for additional information.
The H134R mutation present in ChR2 sequence is a gain-of-function mutant that produces larger stationary photocurrents in comparison to the wild-type ChR2 sequence
Proper citation: RRID:Addgene_20942 Copy
Species: synthetic
Genetic Insert: opto-b2AR
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please see http://www.optogenetics.org
for additional information.
Proper citation: RRID:Addgene_20948 Copy
Species: synthetic
Genetic Insert: opto-a1AR
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please see http://www.optogenetics.org
for additional information.
Proper citation: RRID:Addgene_20947 Copy
Species: C. reinhardtii
Genetic Insert: channelrhodopsin-2
Vector Backbone Description: Backbone Size:5545; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The H134R mutation present in the ChR2 sequence is a gain-of-function mutant that produces larger stationary photocurrents in comparison to the wild-type ChR2 sequence
Proper citation: RRID:Addgene_20940 Copy
Species: V. carteri
Genetic Insert: volvox channelrhodopsin-1
Vector Backbone Description: Backbone Size:5545; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20955 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:7424; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20953 Copy
Species: Mus musculus
Genetic Insert: cMyc, KLF4, Oct4, Sox2
Vector Backbone Description: Backbone Size:9186; Vector Backbone:PB-TET; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Ampicillin
References:
Comments: Additional reference, Kaji et al Nature. 2009 Mar 1
Proper citation: RRID:Addgene_20959 Copy
Species:
Genetic Insert: no insert
Vector Backbone Description: Backbone Size:7480; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: The BamHI site on the full map seen here (based on the author's assembled sequence) is not present in the actual plasmid.
Proper citation: RRID:Addgene_20957 Copy
Species: Discosoma
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFPV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Shaner et al., (2004)
Nature Biotechnology 22,1567-1572
Proper citation: RRID:Addgene_20956 Copy
Species: Xenopus laevis
Genetic Insert: 7XTCF binding sites, Siamois minimal promoter, NLS-lacz
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
References:
Comments: Wnt/beta-catenin responsive promoter in mouse tissues (used to derive the BAT-gal mouse strain), Xenopus embryos and human cell lines
Proper citation: RRID:Addgene_20889 Copy
Species: Mus musculus
Genetic Insert: cMyc
Vector Backbone Description: Backbone Size:9186; Vector Backbone:PB-TET; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20888 Copy
Species: Gloeobacter violaceus PCC 7421
Genetic Insert: pentameric ligand gated ion channel from Gloeobacter violaceus PCC 7421
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pET26b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20887 Copy
Species: Mus musculus
Genetic Insert: p21 3'UTR site 1 and 2 mutation
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-Control; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20880 Copy
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