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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pLV-Hygro-EF1A-EGFP-PARP1 Resource Report Resource Website |
RRID:Addgene_176146 | Poly(ADP-Ribose) Polymerase 1 | Homo sapiens | Ampicillin | PMID:34731617 | Backbone Marker:VectorBuilder; Vector Backbone:Lentivirus; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-01 01:08:53 | 0 | ||
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pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(K35A/K68A/K72/PAMmut) Resource Report Resource Website |
RRID:Addgene_176140 | XRCC1-T2A-PolB | Homo sapiens | Ampicillin | PMID:34731617 | Backbone Marker:VectorBuilder; Vector Backbone:Lentivirus; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | Myc-tagged PolB with mutations Lys35 to Ala, Lys68 to Ala, Lys72 to Ala, and a mutation in the PAM site used by PolBKO gRNA1 | 2026-08-01 01:08:53 | 0 | |
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pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(D256A/PAMmut) Resource Report Resource Website |
RRID:Addgene_176141 | XRCC1-T2A-PolB | Homo sapiens | Ampicillin | PMID:34731617 | Backbone Marker:VectorBuilder; Vector Backbone:Lentivirus; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | Myc-tagged PolB with mutation in Asp256 to Ala, and a mutation in the PAM site used by PolBKO gRNA1 | 2026-08-01 01:08:53 | 0 | |
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EF.CMV.RFP Resource Report Resource Website 10+ mentions |
RRID:Addgene_17619 | Ampicillin | PMID:12788657 | In a SIN LV containing the elongation factor 1alpha promoter, we included a second promoter from cytomegalovirus that drives expression of RFP as a reporter. Dual-promoter LVs can coexpress multiple transgenes efficiently in a single target cell. Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes. There is an a->g mutation in this plasmid that abolishes the HpaI site. A 10bp insertion in Addgene sequence corresponding to position 6893, 21bp insertion in Addgene sequence at position 7405, and 11bp deletion in Addgene sequence at position 7544 do not alter function of the plasmid or RFP expression. The uploaded author's map shows an example with GFP cloned downstream of the EF-1a promoter. The vector supplied by Addgene does not contain the GFP. | Backbone Size:8220; Vector Backbone:EF.CMV.RFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-01 01:08:54 | 15 | |||
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pMC7-XII5dw Resource Report Resource Website |
RRID:Addgene_176177 | 3' homology for integration site XII-5 | Saccharomyces cerevisiae | Chloramphenicol | PMID:34860007 | Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). | Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-01 01:08:54 | 0 | |
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pMC234r-ccdB/TPK2 Resource Report Resource Website |
RRID:Addgene_176179 | dropout cassette containing toxic genes ccdB and TPK2 | Saccharomyces cerevisiae | Chloramphenicol | PMID:34860007 | Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). | Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-01 01:08:54 | 0 | |
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pETx-SR6v15 Resource Report Resource Website |
RRID:Addgene_176212 | SR6v15 | Synthetic | Ampicillin | PMID:34535642 | Backbone Size:5639; Vector Backbone:pETx; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:08:54 | 0 | ||
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pMC6-spacer Resource Report Resource Website |
RRID:Addgene_176178 | 41 bp non-coding DNA spacer from pYTK048 | Synthetic | Chloramphenicol | PMID:34860007 | Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). | Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-01 01:08:54 | 0 | |
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pETx-SR6v15.t Resource Report Resource Website |
RRID:Addgene_176214 | SR6v15.t | Synthetic | Ampicillin | PMID:34535642 | Backbone Size:5639; Vector Backbone:pETx; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:08:54 | 0 | ||
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pETx-SR6v15.d Resource Report Resource Website |
RRID:Addgene_176213 | SR6v15.d | Synthetic | Ampicillin | PMID:34535642 | Backbone Size:5639; Vector Backbone:pETx; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:08:54 | 0 | ||
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pAAV-hAdipoq-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_176215 | GFP | Other | Ampicillin | PMID:32184391 | Backbone Size:5050; Vector Backbone:pAAV-CBA-w; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-08-01 01:08:54 | 1 | ||
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pMC8b-XI3up Resource Report Resource Website |
RRID:Addgene_176170 | 5' homology for integration site XI-3 | Saccharomyces cerevisiae | Chloramphenicol | PMID:34860007 | Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). | Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-01 01:08:54 | 0 | |
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pMC8b-XI5up Resource Report Resource Website |
RRID:Addgene_176172 | 5' homology for integration site XI-5 | Saccharomyces cerevisiae | Chloramphenicol | PMID:34860007 | Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). | Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-01 01:08:54 | 0 | |
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DR.GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_17617 | HLA-DRa promoter | Homo sapiens | Ampicillin | PMID:11781219 | SIN lentiviral vector that expresses GFP controlled by the promoter of the human HLA-DRalpha gene, which is selectively expressed in antigen-presenting cells (APCs). We constructed DR.GFP by using the human (MHC II) HLA-DRalpha promoter (nucleotide 182 to 480, as numbered in GenBank accession X00274) to replace the PGK promoter (XhoI to BamHI sites in the PGK.GFP vector). This 300-base pair promoter sequence has been shown to confer selective expression of a transgene in MHC II+ cells. Recombinant LVs were generated using the 3-plasmid system by cotransfection of 293T cells through calcium phosphate precipitation. The ratio of a transducing vector, pMD.G, and pCMVDeltaR8.91 was fixed at 1.5:0.5:2 µg for 10^6 293T cells. | Backbone Marker:Dr. Didier Trono; Backbone Size:0; Vector Backbone:SIN LV pRLLhPGK.GFP Sin-18 (modified); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-01 01:08:54 | 1 | |
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pMC7-XII2dw Resource Report Resource Website |
RRID:Addgene_176175 | 3' homology for integration site XII-2 | Saccharomyces cerevisiae | Chloramphenicol | PMID:34860007 | Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). | Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-01 01:08:54 | 0 | |
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pMC8b-XII2up Resource Report Resource Website |
RRID:Addgene_176174 | 5' homology for integration site XII-2 | Saccharomyces cerevisiae | Chloramphenicol | PMID:34860007 | Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). | Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-01 01:08:54 | 0 | |
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CAG.mCherry-INBox(AAA)-eDHFR Resource Report Resource Website |
RRID:Addgene_176207 | mCherry-INBoxAAA-eDHFR | Homo sapiens | Ampicillin | PMID:33904910 | Vector Backbone:pEM791; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:08:54 | 0 | ||
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pBx1-VHH template2-3XMyc-Spacer Resource Report Resource Website |
RRID:Addgene_176209 | VHH template2-3XMyc-Spacer | Synthetic | Ampicillin | PMID:34535642 | Backbone Size:2561; Vector Backbone:pBx1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:08:54 | 0 | ||
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pMC8b-XI1up Resource Report Resource Website |
RRID:Addgene_176166 | 5' homology for integration site XI-1 | Saccharomyces cerevisiae | Chloramphenicol | PMID:34860007 | Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). | Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-01 01:08:53 | 0 | |
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CAG.mCherry-eDHFR Resource Report Resource Website |
RRID:Addgene_176201 | mCherry-eDHFR | E. coli | Ampicillin | PMID:33904910 | Please note that there are minor differences between the Addgene NGS result and the depositor's sequence. These differences do not alter plasmid function. | Backbone Marker:Dr. Eugene Makeyev; Backbone Size:-1; Vector Backbone:pEM791; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:08:54 | 0 |
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