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Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF10 protein
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740576
Proper citation: RRID:Addgene_153718 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF6 protein
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740572
Proper citation: RRID:Addgene_153792 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp7
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740578
Proper citation: RRID:Addgene_153798 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp7
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740578
Proper citation: RRID:Addgene_153797 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF6 protein
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740572
Proper citation: RRID:Addgene_153795 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp7
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740578
Proper citation: RRID:Addgene_153724 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 E (envelope)
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740570
Proper citation: RRID:Addgene_153728 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp11
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740578
Proper citation: RRID:Addgene_153720 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp3
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740578. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_153857 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S (surface glycoprotein)
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740568. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_153855 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 2'-O-ribose methyltransferase
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740578. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_153854 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: This plasmid contains a SARS-CoV-2 viral sequence fragment that covers basepairs 8555 to the end of the genome. Because of sequence complexities in nsp3, it is difficult to synthesize the complete nsp3 gene. This construct may be useful to produce RNA controls for assays, as well as serve as templates to make antigens.
Proper citation: RRID:Addgene_153859 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 RNA-dependent RNA polymerase
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: YP_009725307.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_153858 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF10 protein
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740576
Proper citation: RRID:Addgene_153853 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 RNA-dependent RNA polymerase
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740578
Proper citation: RRID:Addgene_153852 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp11
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740578
Proper citation: RRID:Addgene_153850 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF7b
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740574
Proper citation: RRID:Addgene_153909 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop
Vector Backbone Description: Backbone Size:1968; Vector Backbone:pG9m-2 backbone; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid contains a SARS-CoV-2 viral sequence fragment that covers basepairs 8555 to the end of the genome. Because of sequence complexities in nsp3, it is difficult to synthesize the complete nsp3 gene. This construct may be useful to produce RNA controls for assays, as well as serve as templates to make antigens.
During Addgene quality control, an IS3 element and transposase were identified downstream from the SARS-CoV-2 fragment. Requesting scientists should validate that the element has not further transposed into essential elements prior to experimental use.
Proper citation: RRID:Addgene_153907 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S (surface glycoprotein)
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740568. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_153906 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp3
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: YP_009725299.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_153868 Copy
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