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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pET-3c-rhVTN_FL Resource Report Resource Website |
RRID:Addgene_30223 | rhVitronectin without deletions | Homo sapiens | Ampicillin | PMID:21478862 | Note that the first 19 amino acids are the signal peptide for VTN, which is cleaved off on the mature protein during normally protein synthesis in mammalian cells. That means wild type mature VTN starts from aa20. None of the four constructs from this paper have amino acids 1-19. | Backbone Marker:Novagen; Backbone Size:4607; Vector Backbone:pET-3c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | No deletions | Addgene | 2026-11-07 01:13:40 | 0 |
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pTEC22 Resource Report Resource Website 1+ mentions |
RRID:Addgene_30181 | Mycobacterium Strong Promoter (MSP) | M. marinum | Hygromycin | PMID:22840407 | This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with tdKatuschka2. The Katuschka tandem-dimer ORF is present at bp# 44-1522 | Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin | Addgene | 2026-11-07 01:13:39 | 4 | |
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pTEC21 Resource Report Resource Website |
RRID:Addgene_30180 | Mycobacterium Strong Promoter (MSP) | M. marinum | Hygromycin | This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with Kaede. The Kaede ORF is present at bp# 5624-6304. | Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin | Addgene | 2026-11-07 01:13:39 | 0 | ||
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pTEC27 Resource Report Resource Website 10+ mentions |
RRID:Addgene_30182 | Mycobacterium Strong Promoter (MSP) | M. marinum | Hygromycin | PMID:23680983 | This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with tdTomato. The tdTomato ORF is present at bp# 102-1532. | Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin | Addgene | 2026-11-07 01:13:39 | 32 | |
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HL 4218 (=HL 716 + pHL 1411) Resource Report Resource Website |
RRID:Addgene_30105 | pLtetO-1:DsrA, pCon:fhlA:T7noRBS(900bps), pLlacO-1:rpoS::gfp | E. coli | Ampicillin | PMID:21189298 | Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. | Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | Addgene | 2026-11-07 01:13:38 | 0 | |
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pET-3c-rhVTN-NC Resource Report Resource Website 1+ mentions |
RRID:Addgene_30226 | rhVitronectin with N and C terminus cleavages | Homo sapiens | Ampicillin | PMID:21478862 | Note that the first 19 amino acids are the signal peptide for VTN, which is cleaved off on the mature protein during normally protein synthesis in mammalian cells. That means wild type mature VTN starts from aa20. None of the four constructs from this paper have amino acids 1-19. | Backbone Marker:Novagen; Backbone Size:4607; Vector Backbone:pET-3c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | deleted amino acids: 399-478, 20-62 | Addgene | 2026-11-07 01:13:40 | 1 |
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HL 4212 (=HL 716 + pHL 1414) Resource Report Resource Website |
RRID:Addgene_30104 | pCon:fhlA::T7noRBS(900bps), pLlacO-1:rpoS::gfp | E. coli | Ampicillin | PMID:21189298 | Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. | Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | Addgene | 2026-11-07 01:13:38 | 0 | |
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pGEX-2T-IQGAP1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_30107 | IQGAP1 | Homo sapiens | Ampicillin | PMID:9867866 | Backbone Marker:Amersham; Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-11-07 01:13:38 | 1 | ||
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pETCON-E32/design_15 Resource Report Resource Website |
RRID:Addgene_30229 | Mtb ACP2 designed binding protein E32 | Designed Sequence | Ampicillin | PMID:22001016 | Backbone Size:5400; Vector Backbone:pETCON; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-11-07 01:13:40 | 0 | ||
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pGFPHYG2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_30173 | Mycobacterium Strong Promoter (MSP) | M. marinum | Hygromycin | PMID:16714540 | This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. | Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin | GFPmut3 allele has improved brightness. | Addgene | 2026-11-07 01:13:39 | 5 |
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pTEC16 Resource Report Resource Website 1+ mentions |
RRID:Addgene_30175 | Mycobacterium Strong Promoter (MSP) | M. marinum | Hygromycin | PMID:23680983 | This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with mOrange2. The mOrange2 ORF is present at bp# 5620-6330. | Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin | Addgene | 2026-11-07 01:13:39 | 3 | |
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pTEC20 Resource Report Resource Website 1+ mentions |
RRID:Addgene_30179 | Mycobacterium Strong Promoter (MSP) | M. marinum | Hygromycin | PMID:23680983 | This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with mKate2. The mKate2 ORF is present at bp# 105-803. | Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin | Addgene | 2026-11-07 01:13:39 | 3 | |
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pENTR-flbio Resource Report Resource Website |
RRID:Addgene_30216 | No gene | Kanamycin | PMID:21415370 | pENTR3c was re-engineered to allow fusion of flag-bio to the c-terminus of an ORF. The ORF is cloned in 5'-SalI-EcoRI-3'. The ccdb gene was removed. The fusion construct is transferred to a gateway destination vector by LR clonase. | Backbone Size:2400; Vector Backbone:pENTR3C; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | Addgene | 2026-11-07 01:13:40 | 0 | ||
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pET28b-LipB Resource Report Resource Website |
RRID:Addgene_30165 | lipoate protein ligase B | Thermus thermophilus HB27 | Kanamycin | PMID:18076036 | Backbone Size:5500; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-11-07 01:13:39 | 0 | ||
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pET28b-AF0623 Resource Report Resource Website |
RRID:Addgene_30164 | AF0623 | Archaeoglobus fulgidus | Kanamycin | PMID:19478428 | Backbone Size:5500; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-11-07 01:13:39 | 0 | ||
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pMXs-GLIS1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_30166 | GLIS1 | Homo sapiens | Ampicillin | PMID:21654807 | pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H. Gateway casette is from Invitrogen Corporation. IMPORTANT NOTE: This plasmid does not match the canonical GLIS1 sequence. For a more recent GLIS1 plasmid from the Yamanaka lab, please proceed to the following page: www.addgene.org/33274 | Backbone Marker:Shinya Yamanaka; Backbone Size:4700; Vector Backbone:pMXs-gw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | Addgene | 2026-11-07 01:13:39 | 4 | |
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pETCON-HB54/design_7 Resource Report Resource Website |
RRID:Addgene_30274 | SC1918/H1 HA designed binding protein HB54 | Designed Sequence | Ampicillin | PMID:22001016 | Backbone Size:5400; Vector Backbone:pETCON; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-11-07 01:13:40 | 0 | ||
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pETCON-HB58/design_26 Resource Report Resource Website |
RRID:Addgene_30277 | SC1918/H1 HA designed binding protein HB58 | Designed Sequence | Ampicillin | PMID:22001016 | Backbone Size:5400; Vector Backbone:pETCON; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-11-07 01:13:40 | 0 | ||
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pEGFP-C1-PRKAA2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_30310 | AMP-activated Protein Kinase α2 | Rattus norvegicus | Kanamycin | PMID:20685962 | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-11-07 01:13:41 | 1 | ||
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pETCON-HB56/design_34 Resource Report Resource Website |
RRID:Addgene_30276 | SC1918/H1 HA designed binding protein HB56 | Designed Sequence | Ampicillin | PMID:22001016 | Backbone Size:5400; Vector Backbone:pETCON; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-11-07 01:13:40 | 0 |
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