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Species: Homo sapiens
Genetic Insert: rhVitronectin without deletions
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4607; Vector Backbone:pET-3c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21478862
Comments: Note that the first 19 amino acids are the signal peptide for VTN, which is cleaved off on the mature protein during normally protein synthesis in mammalian cells. That means wild type mature VTN starts from aa20. None of the four constructs from this paper have amino acids 1-19.
Proper citation: RRID:Addgene_30223 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:22840407
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with tdKatuschka2. The Katuschka tandem-dimer ORF is present at bp# 44-1522
Proper citation: RRID:Addgene_30181 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with Kaede. The Kaede ORF is present at bp# 5624-6304.
Proper citation: RRID:Addgene_30180 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with tdTomato. The tdTomato ORF is present at bp# 102-1532.
Proper citation: RRID:Addgene_30182 Copy
Species: E. coli
Genetic Insert: pLtetO-1:DsrA, pCon:fhlA:T7noRBS(900bps), pLlacO-1:rpoS::gfp
Vector Backbone Description: Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_30105 Copy
Species: Homo sapiens
Genetic Insert: rhVitronectin with N and C terminus cleavages
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4607; Vector Backbone:pET-3c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21478862
Comments: Note that the first 19 amino acids are the signal peptide for VTN, which is cleaved off on the mature protein during normally protein synthesis in mammalian cells. That means wild type mature VTN starts from aa20. None of the four constructs from this paper have amino acids 1-19.
Proper citation: RRID:Addgene_30226 Copy
Species: E. coli
Genetic Insert: pCon:fhlA::T7noRBS(900bps), pLlacO-1:rpoS::gfp
Vector Backbone Description: Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_30104 Copy
Species: Homo sapiens
Genetic Insert: IQGAP1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9867866
Proper citation: RRID:Addgene_30107 Copy
Species: Designed Sequence
Genetic Insert: Mtb ACP2 designed binding protein E32
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pETCON; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22001016
Proper citation: RRID:Addgene_30229 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:16714540
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance.
Proper citation: RRID:Addgene_30173 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with mOrange2. The mOrange2 ORF is present at bp# 5620-6330.
Proper citation: RRID:Addgene_30175 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with mKate2. The mKate2 ORF is present at bp# 105-803.
Proper citation: RRID:Addgene_30179 Copy
Genetic Insert: No gene
Vector Backbone Description: Backbone Size:2400; Vector Backbone:pENTR3C; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21415370
Comments: pENTR3c was re-engineered to allow fusion of flag-bio to the c-terminus of an ORF. The ORF is cloned in 5'-SalI-EcoRI-3'. The ccdb gene was removed. The fusion construct is transferred to a gateway destination vector by LR clonase.
Proper citation: RRID:Addgene_30216 Copy
Species: Thermus thermophilus HB27
Genetic Insert: lipoate protein ligase B
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18076036
Proper citation: RRID:Addgene_30165 Copy
Species: Archaeoglobus fulgidus
Genetic Insert: AF0623
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19478428
Proper citation: RRID:Addgene_30164 Copy
Species: Homo sapiens
Genetic Insert: GLIS1
Vector Backbone Description: Backbone Marker:Shinya Yamanaka; Backbone Size:4700; Vector Backbone:pMXs-gw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21654807
Comments: pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H. Gateway casette is from Invitrogen Corporation.
IMPORTANT NOTE: This plasmid does not match the canonical GLIS1 sequence. For a more recent GLIS1 plasmid from the Yamanaka lab, please proceed to the following page: www.addgene.org/33274
Proper citation: RRID:Addgene_30166 Copy
Species: Designed Sequence
Genetic Insert: SC1918/H1 HA designed binding protein HB54
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pETCON; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22001016
Proper citation: RRID:Addgene_30274 Copy
Species: Designed Sequence
Genetic Insert: SC1918/H1 HA designed binding protein HB58
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pETCON; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22001016
Proper citation: RRID:Addgene_30277 Copy
Species: Rattus norvegicus
Genetic Insert: AMP-activated Protein Kinase α2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20685962
Proper citation: RRID:Addgene_30310 Copy
Species: Designed Sequence
Genetic Insert: SC1918/H1 HA designed binding protein HB56
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pETCON; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22001016
Proper citation: RRID:Addgene_30276 Copy
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