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Species: E. coli
Genetic Insert: pnos:PAT-tnos
Vector Backbone Description: Backbone Marker:6800; Vector Backbone:pVM_BGW-BpiI; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please note- Functional data on this plasmid can be found in the following publication: Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations. Streubel et al PLoS One. 2017 Mar 16;12(3):e0173580. PMID: 28301511
Proper citation: RRID:Addgene_80585 Copy
Species: E. coli
Genetic Insert: pnos:nptII-tnos
Vector Backbone Description: Backbone Marker:6800; Vector Backbone:pVM_BGW-BpiI; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please note- Functional data on this plasmid can be found in the following publication: Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations. Streubel et al PLoS One. 2017 Mar 16;12(3):e0173580. PMID: 28301511
Proper citation: RRID:Addgene_80584 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2673; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: This plasmid is designed as destination vector for Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using KpnI and EcoRI. The 3' BsaI overhang can be exchanged using XhoI and XbaI. There is also a a NotI-PacI-SgsI-SfaAI-KpnI MCS in front of the A-overhang and an XbaI-PaeI MCS after the G-overhang.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 602-612bp
BsaI site #2 = 2040-2050bp
Proper citation: RRID:Addgene_48869 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2673; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: This plasmid is designed as destination vector for Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using KpnI and EcoRI. The 3' BsaI overhang can be exchanged using XhoI and XbaI. There is also a a NotI-PacI-SgsI-SfaAI-KpnI MCS in front of the A-overhang and an XbaI-PaeI MCS after the G-overhang.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 640-650bp
BsaI site #2 = 2078-2088bp
Proper citation: RRID:Addgene_48868 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtMIR390a-B/c
Vector Backbone Description: Backbone Size:6005; Vector Backbone:pGreen III; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_51777 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pRT100/pPZP312; Vector Types:Binary Gateway vector; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_48257 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pRT100/pPZP312; Vector Types:Binary Gateway vector; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_48255 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pRT100/pPZP312; Vector Types:Binary Gateway vector; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_48256 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-S (pCAMBIA); Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites
Proper citation: RRID:Addgene_54364 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Icon Genetics; Vector Backbone:Xpre-S (pICH50505) ; Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites
Proper citation: RRID:Addgene_54347 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-S (pCAMBIA); Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites
Proper citation: RRID:Addgene_54357 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-S (pCAMBIA); Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites
Proper citation: RRID:Addgene_54356 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:unknown; Vector Backbone:unknown; Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_54404 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Icon Genetics; Vector Backbone:Xpre-S (pICH50505) ; Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: ihpRNA silencing vector (binary expression vector)
Proper citation: RRID:Addgene_54403 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_167299 Copy
Species: Streptococcus pyogenes
Genetic Insert: PcUBQ4-2::Cas9i
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167690 Copy
Species: Staphylococcus aureus
Genetic Insert: PcUBQ4-2::asCpf1
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167693 Copy
Species: Staphylococcus aureus
Genetic Insert: pEC1.2::asCpf1
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167692 Copy
Species: Streptococcus pyogenes
Genetic Insert: pEC1.2::Cas9i
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167691 Copy
Species: Streptococcus pyogenes
Genetic Insert: PcUBQ4-2::Cas9i
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167687 Copy
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