Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Vector Backbone Description: Backbone Marker:Novagen, EMBL; Backbone Size:6841; Vector Backbone:pET, pETM44; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43986 Copy
Vector Backbone Description: Backbone Marker:Novagen, EMBL; Backbone Size:7237; Vector Backbone:pET, pETM14; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43987 Copy
Species: Homo sapiens
Genetic Insert: USP44
Vector Backbone Description: Backbone Size:9600; Vector Backbone:pDEST-MYC; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17443180
Proper citation: RRID:Addgene_43901 Copy
Vector Backbone Description: Backbone Marker:Novagen, EMBL; Backbone Size:6660; Vector Backbone:pET, pET28M-Sumo1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43989 Copy
Species: Homo sapiens
Genetic Insert: USP44
Vector Backbone Description: Backbone Size:4700; Vector Backbone:p142; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17443180
Proper citation: RRID:Addgene_43903 Copy
Species: Homo sapiens
Genetic Insert: USP44
Vector Backbone Description: Backbone Size:9692; Vector Backbone:p332; Vector Types:Insect Expression, baculoviral expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17443180
Proper citation: RRID:Addgene_43905 Copy
Genetic Insert: AGA1
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9181578
Proper citation: RRID:Addgene_44036 Copy
Vector Backbone Description: Backbone Marker:Novagen,; Backbone Size:6091; Vector Backbone:pET, pCoofy2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43982 Copy
Species: Homo sapiens
Genetic Insert: 5'UTR-Tax-3'UTR
Vector Backbone Description: Backbone Marker:Dr. Inder Verma; Vector Backbone:pCLXSN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11553609
Comments: Transfer of BamHI-BamHI fragment covering 5'UTR-Tax-3'UTR from pCMV4-Tax (from Dr. Warner Greene) to the BamHI site of pCLXSN (from Dr. Verma Inder).
Proper citation: RRID:Addgene_44038 Copy
Vector Backbone Description: Backbone Marker:Novagen, EMBL; Backbone Size:6406; Vector Backbone:pET, pETM33; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Note that the 6x His GST double tag tends to increase the formation of insoluble protein aggregates.
Proper citation: RRID:Addgene_43983 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5749; Vector Backbone:pET, pCoofy1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Note that depending on the protein, the 10x His tag can reduce or abolish expression.
Proper citation: RRID:Addgene_43975 Copy
Vector Backbone Description: Backbone Marker:Novagen, EMBL; Backbone Size:5752; Vector Backbone:pET, pETM14; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43976 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5809; Vector Backbone:pET, pCoofy1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43977 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5842; Vector Backbone:pET, pCoofy12; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43979 Copy
Vector Backbone Description: Backbone Size:5500; Vector Backbone:p416-GPD; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21586584
Proper citation: RRID:Addgene_43972 Copy
Vector Backbone Description: Backbone Marker:Novagen, EMBL; Backbone Size:5737; Vector Backbone:pET, pETM14; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43974 Copy
Species: Synthetic
Genetic Insert: HRP
Vector Backbone Description: Backbone Size:6142; Vector Backbone:pCTcon2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17961829
Proper citation: RRID:Addgene_44021 Copy
Vector Backbone Description: Backbone Marker:http://www.arabidopsis.org/servlet/TairObject?id=500300075&type=vector; Backbone Size:11405; Vector Backbone:pFGC5941; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23098881
Proper citation: RRID:Addgene_44020 Copy
Genetic Insert: puroR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2670; Vector Backbone:pDONR221-P4rP3r; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23637962
Proper citation: RRID:Addgene_43965 Copy
Genetic Insert: pA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2557; Vector Backbone:pDONR221-P3P2; Vector Types:Multisite Gateway ENTR clone; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23637962
Proper citation: RRID:Addgene_43966 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the NIF Resources search. From here you can search through a compilation of resources used by NIF and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that NIF has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on NIF then you can log in from here to get additional features in NIF such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into NIF you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within NIF that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
Welcome to the Neuroscience Information Framework Project, designed to serve the biomedical research community. NIF maintains the largest searchable collection of neuroscience data, the largest catalog of biomedical resources, and the largest ontology for neuroscience on the web. We welcome all feedback and suggestions and are actively looking for resource providers to make their resources accessible through NIF. Learn about the tools available to help you share your data and discover a dynamic inventory of Web-based neuroscience resources.
