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Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74800 Copy
Species: Homo sapiens
Genetic Insert: SLC2A3
Vector Backbone Description: Vector Backbone:pFB-CT10HF-LIC; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin
Comments: Baculo vector with C-terminal 10His+flag, TEV-cleavable, for LIC cloning. gi|5902090
Proper citation: RRID:Addgene_74649 Copy
Species: Homo sapiens
Genetic Insert: JARID1BA
Vector Backbone Description: Vector Backbone:pFB-LIC-Bse; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin
Comments: Baculovirus transfer vector (Bac-to-bac), N-terminal His-tag, TEV cleavable. gi|19923370
Proper citation: RRID:Addgene_74648 Copy
Species: Homo sapiens
Genetic Insert: TIF1A
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: T7/lac regulated, N-terminal His-tag, SUMO, restriction cloned with NdeI/XhoI gi|14971415
Proper citation: RRID:Addgene_74647 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74796 Copy
Species: Homo sapiens
Genetic Insert: HO1
Vector Backbone Description: Backbone Marker:Giovannoni lab, Addgene plasmid 74670; Vector Backbone:pCX-2A-2A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26513260
Comments: Contains a D473N mutation in CD73, but it does not impair either the plasmid function or the hCD73 protein activity.
Proper citation: RRID:Addgene_74674 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Comments: Please note that pUC primers are not recommended for sequencing due to the inverted repeat of the Tnos sequence which forms a secondary structure and prevents sequencing reactions. If you need to sequence or PCR the Tnos region, digestion by AscI helps separates two Tnos sequences and prevents the secondary structure.
Proper citation: RRID:Addgene_74795 Copy
Species: E. coli
Genetic Insert: PTac-RBSB2-BM3R1-PBM3R1-YFP
Vector Backbone Description: Backbone Marker:Alec Nielsen; Backbone Size:3930; Vector Backbone:pAN801; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27034378
Proper citation: RRID:Addgene_74679 Copy
Species: E. coli
Genetic Insert: PTac-RBSB1-BM3R1-PBM3R1-YFP
Vector Backbone Description: Backbone Marker:Alec Nielsen; Backbone Size:3930; Vector Backbone:pAN801; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27034378
Proper citation: RRID:Addgene_74678 Copy
Species: E. coli
Genetic Insert: PTac-RBSE1-BetI-PBetI-YFP
Vector Backbone Description: Backbone Marker:Alec Nielsen; Backbone Size:3930; Vector Backbone:pAN801; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27034378
Proper citation: RRID:Addgene_74677 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74798 Copy
Species: E. coli
Genetic Insert: PTac-RBSA1-AmtR-PAmtR-YFP
Vector Backbone Description: Backbone Marker:Alec Nielsen; Backbone Size:3930; Vector Backbone:pAN801; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27034378
Proper citation: RRID:Addgene_74676 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74797 Copy
Species: Homo sapiens
Genetic Insert: PKU1
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. gi|4557819
Proper citation: RRID:Addgene_74663 Copy
Species: Homo sapiens
Genetic Insert: Mmadhc
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. gi|19527054
Proper citation: RRID:Addgene_74662 Copy
Species: Chaetomium thermophilum
Genetic Insert: Chaetomium thermophilum dynein light intermediate chain
Vector Backbone Description: Backbone Size:5385; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25272277
Proper citation: RRID:Addgene_74782 Copy
Species: synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_74668 Copy
Species: Homo sapiens
Genetic Insert: PHIPA
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. gi|34996489
Proper citation: RRID:Addgene_74665 Copy
Species: E. coli
Genetic Insert: PTac-RBSS3-SrpR-PSrpR-YFP
Vector Backbone Description: Backbone Marker:Alec Nielsen; Backbone Size:3930; Vector Backbone:pAN801; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27034378
Proper citation: RRID:Addgene_74693 Copy
Species: E. coli
Genetic Insert: PTac-RBSQ2-QacR-PQacR-YFP
Vector Backbone Description: Backbone Marker:Alec Nielsen; Backbone Size:3930; Vector Backbone:pAN801; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27034378
Proper citation: RRID:Addgene_74690 Copy
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