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Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:9528; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: MIGS vectors are based on pGREEN series:
Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000)
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant
transformation. Plant Mol. Biol., 42, 819-832.
http://www.pgreen.ac.uk/
obtained from John Innes Centre, UK
Proper citation: RRID:Addgene_35248 Copy
Species: Synthetic
Genetic Insert: sgRNA-25xPBS9R
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8GWTOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.03.974923v2.full for bioRxiv preprint.
Proper citation: RRID:Addgene_183217 Copy
Species: Synthetic
Genetic Insert: sgRNA-20xPBS48107
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8GWTOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.03.974923v2.full for bioRxiv preprint.
Proper citation: RRID:Addgene_183215 Copy
Species: Synthetic
Genetic Insert: sgRNA-20xPBSc
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8GWTOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.03.974923v2.full for bioRxiv preprint.
Proper citation: RRID:Addgene_183216 Copy
Species: Synthetic
Genetic Insert: sgRNA-15xPBSc
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8GWTOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.03.974923v2.full for bioRxiv preprint.
Proper citation: RRID:Addgene_183213 Copy
Species: Synthetic
Genetic Insert: sgRNA-15xPBS9R
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8GWTOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.03.974923v2.full for bioRxiv preprint.
Proper citation: RRID:Addgene_183214 Copy
Species: Synthetic
Genetic Insert: sgRNA-15xPBSa
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8GWTOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_183346 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5008; Vector Backbone:pDONR223_P2rP4; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Modified version of pDONR223_P12 from Invitrogen.
Recombine Gateway compatibile PCR product (attB2r - attB4) into this Entry vector by Gateway BP reaction.
The pFUSE-A_P1P2 is the same plasmid as Gateway™ pDONR™221 Vector and is commercially available from ThermoFisher Scientific (Cat#: 12536017).
Proper citation: RRID:Addgene_97185 Copy
Species:
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pVM_BGW; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_79440 Copy
Species:
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pVM_BGW-BpiI; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_79447 Copy
Species:
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_79448 Copy
Species:
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pVM_BGW-BpiI; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_79443 Copy
Species:
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pVM_BGW-BpiI; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_79442 Copy
Species:
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_79450 Copy
Species:
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_79452 Copy
Species:
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_79455 Copy
Species:
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_79458 Copy
Species:
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_79456 Copy
Species: E. coli
Genetic Insert: pnos:nptII-tmos
Vector Backbone Description: Backbone Marker:6800; Vector Backbone:pVM_BGW; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_80578 Copy
Species: E. coli
Genetic Insert: pnos:PAT-tnos
Vector Backbone Description: Backbone Marker:6800; Vector Backbone:pVM_BGW; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_80581 Copy
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