Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Vector Backbone Description: Backbone Marker:John Mekalanos lab, Harvard Medical School (PMID: 2836362); Backbone Size:5720; Vector Backbone:pGP704; Vector Types:Bacterial Expression, Bacterial allelic exchange vector with sacB1; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9511756
Comments: The plasmids deposited here comprise a set of SacB1-dependent allelic exchange vectors improved from a previously described suicide vector, pGP704 (Miller and Mekalanos, 1988), by including a system to select for plasmid loss by recombination.
Plasmid pRE107 was constructed by cloning the appropriate EcoRI fragment from pUC58-sacB1 (McIver et al., 1995) into pGP704 (see associated schematic image). The sacB1 allele is a modified variation of sacB, where unique restriction sites were removed by site directed mutagenesis (McIver et al., 1995). Additionally, the BamHI site in the R6K ori of the resulting plasmid was removed by partial BamHI digestion, blunting the resulting overhangs with PolIk (resulting in the formation of a ClaI site) and screening for its loss by restriction analysis.
The resulting plasmid together with others in this deposited series contain the conditional R6K ori, the origin of transfer (oriT) which allows conjugative transfer from permissive hosts, the sacB1 gene to provide negative selection and a MCS, along with a range of different AbR markers.
Note: Addgene's NGS results found that a large portion of the plasmid backbone (containing the oriT feature) is in the opposite orientation compared to the depositor's reference sequence.
Proper citation: RRID:Addgene_43829 Copy
Species: Synthetic
Genetic Insert: IV-TGC
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43788 Copy
Species: Synthetic
Genetic Insert: IV-TAC
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43780 Copy
Species: Synthetic
Genetic Insert: IV-TCC
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43784 Copy
Species: Synthetic
Genetic Insert: IV-TCG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43785 Copy
Species: Synthetic
Genetic Insert: IV-TCT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43786 Copy
Species: Synthetic
Genetic Insert: IV-TGA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43787 Copy
Species: Synthetic
Genetic Insert: IV-GTG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43777 Copy
Species: Synthetic
Genetic Insert: IV-GTT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43778 Copy
Species: Synthetic
Genetic Insert: IV-TAA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43779 Copy
Genetic Insert: TALE-activator p65 backbone
Vector Backbone Description: Backbone Marker:Joung lab; Vector Backbone:unknown; Vector Types:TALE Transcriptional Activator Expression Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23396285
Comments: Location of plasmid features:
EF1a = 922-2106
start codon = 2119-2121
NLS = 2122-2165
N-term = 2166-2577
0.5 domain = 2592-2658
C-term = 2665-2949
p65 = 2959-3768
pA = 3775-3894
Proper citation: RRID:Addgene_43891 Copy
Species: Synthetic
Genetic Insert: IV-GGA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43771 Copy
Species: Synthetic
Genetic Insert: IV-GGT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43774 Copy
Species: Synthetic
Genetic Insert: IV-GTA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43775 Copy
Species: Synthetic
Genetic Insert: IV-GCA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43767 Copy
Genetic Insert: TALE-activator p65 backbone
Vector Backbone Description: Backbone Marker:Joung lab; Vector Backbone:unknown; Vector Types:TALE Transcriptional Activator Expression Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23396285
Comments: Location of plasmid features:
EF1a = 922-2106
start codon = 2119-2121
NLS = 2122-2165
N-term = 2166-2577
0.5 domain = 2592-2658
C-term = 2665-2949
p65 = 2959-3768
pA = 3775-3894
Proper citation: RRID:Addgene_43889 Copy
Species: Homo sapiens
Genetic Insert: Human Optimized S. pyogenes Cas9
Vector Backbone Description: Backbone Size:6670; Vector Backbone:p415; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23460208
Proper citation: RRID:Addgene_43804 Copy
Genetic Insert: SID4X Domain
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22828628
Comments: This enhanced SID domain (SID4X domain) is to be used with TALE effectors to create TALE repressors, as described in Cong et al. Nature Communications. This new domain replaces the previous SID domain reported in the original article.
Proper citation: RRID:Addgene_43882 Copy
Genetic Insert: TALE-activator VP64 Backbone
Vector Backbone Description: Backbone Marker:Joung lab; Vector Backbone:unknown; Vector Types:TALE Transcriptional Activator Expression Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23396285
Comments: Location of plasmid features:
EF1a = 922-2106
start codon - 2119-2121
NLS = 2122-2165
N-term =2166-2577
0.5 domain = 2592-2658
C-term = 2665-2949
VP64 = 2956-3122
pA = 3125-3244
Proper citation: RRID:Addgene_43885 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: DIF1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18851834
Comments: The following mutations were found during the Addgene QC sequencing: Q31 deletion, S60G and Q68H.
Proper citation: RRID:Addgene_43909 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the NIF Resources search. From here you can search through a compilation of resources used by NIF and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that NIF has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on NIF then you can log in from here to get additional features in NIF such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into NIF you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within NIF that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
Welcome to the Neuroscience Information Framework Project, designed to serve the biomedical research community. NIF maintains the largest searchable collection of neuroscience data, the largest catalog of biomedical resources, and the largest ontology for neuroscience on the web. We welcome all feedback and suggestions and are actively looking for resource providers to make their resources accessible through NIF. Learn about the tools available to help you share your data and discover a dynamic inventory of Web-based neuroscience resources.
