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Species: Acetobacter aceti 1023
Genetic Insert: formylglycinamidine ribonucleotide synthetase, glutaminase subunit
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_74225 Copy
Species: Homo sapiens
Genetic Insert: human AMPK alpha 1 exon1 gRNA
Vector Backbone Description: Backbone Marker: Addgene plasmid 48141; Vector Backbone:pX462; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26816379
Proper citation: RRID:Addgene_74374 Copy
Genetic Insert: HA
Vector Backbone Description: Vector Backbone:pCB6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10436026
Proper citation: RRID:Addgene_74373 Copy
Species: Mus musculus
Genetic Insert: mouse Opn
Vector Backbone Description: Vector Backbone:pBABE.hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_74411 Copy
Species: Homo sapiens
Genetic Insert: human AMPK alpha 2 exon1 gRNA
Vector Backbone Description: Backbone Marker: Addgene plasmid 48141; Vector Backbone:pX462; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26816379
Proper citation: RRID:Addgene_74377 Copy
Species: Homo sapiens
Genetic Insert: human AMPK alpha 1 exon1 gRNA
Vector Backbone Description: Backbone Marker: Addgene plasmid 48141; Vector Backbone:pX462; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26816379
Proper citation: RRID:Addgene_74375 Copy
Species: Mus musculus
Genetic Insert: teashirt zinc finger family member 1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM-T easy; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27243442
Comments: SacII digestion and SP6 RNA polymerase for antisense probe
Proper citation: RRID:Addgene_74360 Copy
Species: Mus musculus
Genetic Insert: zinc fingers and homeoboxes 3
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM-T easy; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27243442
Comments: SpeI digestion and T7 RNA polymerase for antisense probe
Proper citation: RRID:Addgene_74366 Copy
Species: Mus musculus
Genetic Insert: zinc fingers and homeoboxes 2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM-T easy; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27243442
Comments: NcoI digestion and SP6 RNA polymerase for antisense probe
Proper citation: RRID:Addgene_74365 Copy
Species: Mus musculus
Genetic Insert: zinc fingers and homeoboxes 1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM-T easy; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27243442
Comments: NcoI digestion and SP6 RNA polymerase for antisense probe
Proper citation: RRID:Addgene_74364 Copy
Species: Synthetic
Genetic Insert: RGR-r11
Vector Backbone Description: Backbone Marker:Klavins Lab; Backbone Size:4629; Vector Backbone:pMOD8; Vector Types:Yeast Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/early/2016/03/02/041871 for bioRxiv preprint.
Proper citation: RRID:Addgene_74369 Copy
Species: rabbit
Genetic Insert: SERCA1
Vector Backbone Description: Backbone Marker:Invitrogen ; Backbone Size:5514; Vector Backbone:pcDNA3.1/HisA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_74402 Copy
Species: Homo sapiens
Genetic Insert: S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2)
Vector Backbone Description: Backbone Size:4246; Vector Backbone:PX462; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26969735
Comments: The catalytic domain of human DNMT3A (amino acids P602-V912) was derived from the plasmid pcDNA3/Myc-DNMT3A (Addgene, plasmid #35521) (Chen et al., 2005, J Cell Biochem 95: 902-917). Undesired BbsI restriction site was removed by site-directed mutagenesis, without affecting the amino acid sequence. The DNMT3A active site motif ENV was mutated to ANV (E756A).
Plasmid pSpCas9n(BB)-2A-Puro (PX462) (Addgene, plasmid #48141) (Ran et al., 2013, Nat Protoc 8: 2281-2308) was used as a backbone. Additional H840A mutation was introduced into Cas9n D10A nickase.
Puromycin gene was mutated by site-directed mutagenesis (H166R) to improve its efficacy.
Proper citation: RRID:Addgene_74408 Copy
Species: Homo sapiens
Genetic Insert: Flag-MFF
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26816379
Proper citation: RRID:Addgene_74392 Copy
Species: Mus musculus
Genetic Insert: stathmin-like 2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM-T easy; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27521061
Comments: ApaI digestion and SP6 RNA polymerase for antisense probe
Proper citation: RRID:Addgene_74397 Copy
Species: Danio rerio
Genetic Insert: zebrafish PSD95
Vector Backbone Description: Backbone Marker:clontech/invitrogen; Backbone Size:5; Vector Backbone:pEGFP-N2; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14758365
Comments: The depositor noted that the Y599H and I793S mutations found in Addgene's quality control sequence does NOT affect plasmid function.
Proper citation: RRID:Addgene_74315 Copy
Species: Danio rerio
Genetic Insert: zebrafish PSD95
Vector Backbone Description: Backbone Marker:Clontech/Invitrogen; Backbone Size:5; Vector Backbone:pEGFP-N2; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14758365
Comments: The depositor noted that the Y599H and I793S mutations found in Addgene's quality control sequence does NOT affect plasmid function.
Proper citation: RRID:Addgene_74314 Copy
Species: Danio rerio
Genetic Insert: synaptophysin
Vector Backbone Description: Vector Backbone:pEGFP-N2; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16571769
Proper citation: RRID:Addgene_74317 Copy
Species: Homo sapiens
Genetic Insert: Flag-MFF
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26816379
Proper citation: RRID:Addgene_74381 Copy
Species: Homo sapiens
Genetic Insert: Flag-MFF
Vector Backbone Description: Backbone Marker: Addgene plasmid 17400; Vector Backbone:pQCXIB CMV/TO DEST (w320-1); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26816379
Proper citation: RRID:Addgene_74385 Copy
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