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Species: Homo sapiens
Genetic Insert: Glutaredoxin-1
Vector Backbone Description: Backbone Marker:Qiagen; Vector Backbone:pQE-60; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18469822
Comments: Discrepancies between the QC and full sequence have no functional consequence.
Proper citation: RRID:Addgene_64799 Copy
Species: Mus musculus
Genetic Insert: Akt1
Vector Backbone Description: Backbone Size:8892; Vector Backbone:pLVX-IRES-tdTomato; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25856301
Proper citation: RRID:Addgene_64831 Copy
Species: Homo sapiens
Genetic Insert: miR-34a promoter P6
Vector Backbone Description: Backbone Marker:Joshua Mendell; Backbone Size:5408; Vector Backbone:pGL3-Basic-IRES; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17540599
Proper citation: RRID:Addgene_64790 Copy
Genetic Insert: tnsABCD
Vector Backbone Description: Vector Backbone:unknown; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15908923
Proper citation: RRID:Addgene_64967 Copy
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18R6KT-mini-Tn7T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:15908923
Comments: The uncut plasmid runs as a multimer (>10kb). Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. If the monomer is needed, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_64969 Copy
Genetic Insert: oriR6K
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18T-mini-Tn7T-Gm; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:15908923
Comments: Genbank Accession #AY884833
Proper citation: RRID:Addgene_64966 Copy
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18-mini-Tn7T-Gm; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Gentamicin
Defining Citation: PMID:15908923
Comments: GenBank Accession # AY737004
Proper citation: RRID:Addgene_64961 Copy
Species: Staphylococcus aureus ATCC 10832
Genetic Insert: sortase A
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6688; Vector Backbone:pTWIN1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22729808
Proper citation: RRID:Addgene_64973 Copy
Vector Backbone Description: Backbone Size:3000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology, E. coli; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25982507
Proper citation: RRID:Addgene_64909 Copy
Vector Backbone Description: Backbone Size:3500; Vector Backbone:ColE (from pZ system) + KanR; Vector Types:Bacterial Expression, Synthetic Biology, E. coli; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25982507
Proper citation: RRID:Addgene_64906 Copy
Vector Backbone Description: Backbone Size:3000; Vector Backbone:ColE (from pZ system) + KanR; Vector Types:Bacterial Expression, Synthetic Biology, E. coli; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25982507
Proper citation: RRID:Addgene_64905 Copy
Vector Backbone Description: Backbone Size:3500; Vector Backbone:ColE (from pZ system) + KanR; Vector Types:Bacterial Expression, Synthetic Biology, E. coli; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25982507
Proper citation: RRID:Addgene_64908 Copy
Species: Staphylococcus aureus ATCC 10832
Genetic Insert: sortase A
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6688; Vector Backbone:pTWIN1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22729808
Proper citation: RRID:Addgene_64981 Copy
Species: Staphylococcus aureus ATCC 10832
Genetic Insert: sortase A
Vector Backbone Description: Backbone Marker:IBA; Backbone Size:3211; Vector Backbone:pASK-IBA3 plus; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25864513
Proper citation: RRID:Addgene_64983 Copy
Species: Staphylococcus aureus ATCC 10832
Genetic Insert: sortase A
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6688; Vector Backbone:pTWIN1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22729808
Proper citation: RRID:Addgene_64980 Copy
Species: Synthetic
Genetic Insert: hsGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3954; Vector Backbone:pBAD/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25141269
Comments: Please cite: Z. Chen and H-w. Ai*, Biochemistry, 2014, 53: 5966-5974; and S. Chen, Z. Chen, W. Ren and H-w. Ai*, J. Am. Chem. Soc., 2012, 134: 9589-9592. For E. coli expression, you will also need pEVOL-pAzF (Addgene # 31186): https://www.addgene.org/31186/
Proper citation: RRID:Addgene_64911 Copy
Species: Homo sapiens
Genetic Insert: Glutaredoxin-1
Vector Backbone Description: Vector Backbone:pCaSpeR4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22100409
Comments: Sequence discrepancies found during QC should not affect function
Proper citation: RRID:Addgene_64999 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Orp1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pLPCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19755417
Proper citation: RRID:Addgene_64992 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Orp1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pLPCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19755417
Proper citation: RRID:Addgene_64991 Copy
Species: Homo sapiens
Genetic Insert: Glutaredoxin-1
Vector Backbone Description: Vector Backbone:pEIGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18469822
Comments: Sequence discrepancies found during QC should not affect function
Proper citation: RRID:Addgene_64990 Copy
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