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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Authority Record Last Update Mentions Count
pflaG
 
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RRID:Addgene_45477 flaA-GFP Ampicillin PMID:10447882 pflaG was generated by subcloning the 0.8kb XbaI/PstI fragment encoding GFP and T7 gene 10 rbs from pGFPmut3 into pJB98. The flaA promoter including its -35, -10, and transcriptional start sites were amplified from the chromosomal DNA of L. pneumophila 02 and ligated directly upstream of GFP using BamHI/XbaI. pJB98 was generated from pKB5 (Berger and Isberg, 1993) by deletion of ~1.5kb HpaI/EcoRI fragment containing the ptac promoter and lacIq gene. Vector Backbone:pJB98; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Addgene 2026-11-07 01:15:46 0
pTLPflaG-small
 
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RRID:Addgene_45476 flaA-GFP Chloramphenicol PMID:10447882 The ~1kb BamHI/SphI flaAGFP fragment from pflaG into the ~4.6kb BamHI/SphI fragment of the ColE1 replicon pTLP6. Backbone Size:4600; Vector Backbone:pTLP6; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol Addgene 2026-11-07 01:15:46 0
HB101 endA::frt lambda pir
 
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RRID:Addgene_45472 endA::frt lambda pir Streptomycin PMID:21306445 Use lambda pir strain for R6K ori replication. endA was deleted for cleaner and higher yield plasmid preps. Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin major endonuclease endA deleted Addgene 2026-11-07 01:15:46 0
pCELF PAI-RBP v2
 
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RRID:Addgene_45505 PAI-RBP v2 Homo sapiens Ampicillin PMID:11001948 Our lab isolated the protein based on it's ability to bind to a region of the PAI-1 (plasminogen activator inhibitor type 1) 3'UTR that we had determined to be involved in the cAMP regulation of mRNA stability. A search of the database revealed that the cDNA for the protein had been cloned by the German Genome Project and dubbed hypothetical protein. They kindly provided us with the clone, which we subcloned into pET-15b for expression of the protein and then into CMV-Flag for mammalian expression. Subsequently, we discovered isoforms of the protein with or without 6 and/or 15 amino acid insertions. Variant 2 has the 6 amino acid insertion but lacks the 15 amino acid insertion. Additional reference: Heaton, JH et al 2003 Thromb Haemost 89; 959-966. Backbone Size:6000; Vector Backbone:pCEFL; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-11-07 01:15:46 0
Tol2 Krt4 Src (YF)-uniRapR-cerulean
 
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RRID:Addgene_45509 Src (YF)-uniRapR-cerulean Ampicillin PMID:23569285 Please note that Addgene's sequencing results differ from the full plasmid sequence information from the depositing laboratory at bp# 3150, 3188, 3193, 3199, 3211, 3227, 3307, 5577 & 5578. These differences are not a concern for the function of the plasmid according to the depositing laboratory. Backbone Size:9773; Vector Backbone:pT2KHspGGFF (Asakawa K, Kawakami K. Dev Growth Differ. 2008); Vector Types:zebrafish expression; Bacterial Resistance:Ampicillin Y732F Addgene 2026-11-07 01:15:46 0
pMito-iRFP720
 
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RRID:Addgene_45464 iRFP720 Rhodopseudomonas palustris Kanamycin PMID:23770755 Backbone Marker:Clontech; Backbone Size:4038; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193K, F198Y, D202F, V254N, Y258F, A283V, K288T, N290Y relative to RpBphP2 Addgene 2026-11-07 01:15:46 0
pNLS-iRFP670
 
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1+ mentions
RRID:Addgene_45466 iRFP670 Rhodopseudomonas palustris Kanamycin PMID:23770755 Backbone Marker:Clontech; Backbone Size:4055; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin T2A, S45T, V116I, D124E, M170L, K180M, D202L, I203V, I219V, R233H, V251I, V254C, Y258F, A283I, F307L relative to RpBphP6 Addgene 2026-11-07 01:15:46 8
pMito-iRFP713
 
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1+ mentions
RRID:Addgene_45465 iRFP713 Rhodopseudomonas palustris Kanamycin PMID:23770755 For more information on in vivo imaging, please see: https://www.addgene.org/fluorescent_proteins/in_vivo/ Backbone Marker:Clontech; Backbone Size:4038; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T, N290Y relative to RpBphP2 Addgene 2026-11-07 01:15:46 2
pcDNA3.1-Flag-PGC-1alpha1
 
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1+ mentions
RRID:Addgene_45501 PGC-1alpha Isoform1 Mus musculus Ampicillin PMID:23217713 Note that Addgene NGS results identified a Q339K mutation relative to the canonical GenBank sequence. This mutation has not been reported to affect function. Backbone Size:5424; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Q339K relative to NM_008904 Addgene 2026-11-07 01:15:46 1
pR6Kfrt-gent-frt
 
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RRID:Addgene_45469 frt-gent-frt Gentamycin PMID:21296953 frt-gent-frt cassette was cut out of pGEM-frt-gent-frt with EcoRI and ligated into EcoRI digested pR6K. Please note that the R6K ori may be in opposite orientation relative to cassette and annotation since it was not directionally cloned. Backbone Marker:Datensko and Wanner; Vector Backbone:pR6K; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamycin Addgene 2026-11-07 01:15:46 0
pBAD/HisB-iRFP720
 
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1+ mentions
RRID:Addgene_45460 iRFP720 Rhodopseudomonas palustris Ampicillin PMID:23770755 Backbone Marker:Invitrogen; Backbone Size:4067; Vector Backbone:pBAD/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193K, F198Y, D202F, V254N, Y258F, A283V, K288T, N290Y relative to RpBphP2 Addgene 2026-11-07 01:15:46 1
pMito-iRFP670
 
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1+ mentions
RRID:Addgene_45462 iRFP670 Rhodopseudomonas palustris Kanamycin PMID:23770755 Backbone Marker:Clontech; Backbone Size:4038; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin T2A, S45T, V116I, D124E, M170L, K180M, D202L, I203V, I219V, R233H, V251I, V254C, Y258F, A283I, F307L relative to RpBphP6 Addgene 2026-11-07 01:15:46 2
pBAD/HisB-iRFP702
 
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1+ mentions
RRID:Addgene_45455 iRFP702 Rhodopseudomonas palustris Ampicillin PMID:23770755 Backbone Marker:Invitrogen; Backbone Size:4074; Vector Backbone:pBAD/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin T2A, S45T, D124E, M170L, D202L, I203V, I219V, R233H, V251I, Y258F, A283I, F307L relative to RpBphP6 Addgene 2026-11-07 01:15:46 1
piRFP670-N1
 
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50+ mentions
RRID:Addgene_45457 iRFP670 Rhodopseudomonas palustris Kanamycin PMID:23770755 For more information on in vivo imaging, please see: https://www.addgene.org/fluorescent_proteins/in_vivo/ Backbone Marker:Clontech; Backbone Size:4012; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin T2A, S45T, V116I, D124E, M170L, K180M, D202L, I203V, I219V, R233H, V251I, V254C, Y258F, A283I, F307L relative to RpBphP6 Addgene 2026-11-07 01:15:46 57
pWzl_neo_DEST_flag_hcRED
 
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RRID:Addgene_45458 hcRED Ampicillin PMID:23764425 Backbone Size:5500; Vector Backbone:pWzl-neo-flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin Addgene 2026-11-07 01:15:46 0
pBbA5k-EPL124
 
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RRID:Addgene_45441 acrD Synthetic Kanamycin PMID:21556065 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Vector Backbone:pBbA5k; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin Addgene 2026-11-07 01:15:46 0
IF-GFP-ATRX
 
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1+ mentions
RRID:Addgene_45444 ATRX Homo sapiens Kanamycin PMID:24834375 The EcoRI restriction pattern is different between the plasmid described in the paper and the plasmid available from Addgene. This difference does not effect the function of the plasmid. Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP C2; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin isoform 2, missing codon E124 Addgene 2026-11-07 01:15:46 7
pCI-EGFP-NR1 wt
 
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1+ mentions
RRID:Addgene_45446 NR1 Rattus norvegicus Ampicillin PMID:12160751 Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin EGFP inserted after the predicted signal peptide cleavage site (after amino acid residue 26) Addgene 2026-11-07 01:15:46 7
pCI-EGFP-NR2a wt
 
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1+ mentions
RRID:Addgene_45445 NR2A Rattus norvegicus Ampicillin PMID:12160751 Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin EGFP inserted after the predicted signal peptide cleavage site (after amino acid residue 21) Addgene 2026-11-07 01:15:46 5
pBbA5k-EPL101
 
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RRID:Addgene_45438 Shal_1195 Synthetic Kanamycin PMID:21556065 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Vector Backbone:pBbA5k; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin Addgene 2026-11-07 01:15:46 0

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