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Genetic Insert: flaA-GFP
Vector Backbone Description: Vector Backbone:pJB98; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10447882
Comments: pflaG was generated by subcloning the 0.8kb XbaI/PstI fragment encoding GFP and T7 gene 10 rbs from pGFPmut3 into pJB98. The flaA promoter including its -35, -10, and transcriptional start sites were amplified from the chromosomal DNA of L. pneumophila 02 and ligated directly upstream of GFP using BamHI/XbaI.
pJB98 was generated from pKB5 (Berger and Isberg, 1993) by deletion of ~1.5kb HpaI/EcoRI fragment containing the ptac promoter and lacIq gene.
Proper citation: RRID:Addgene_45477 Copy
Genetic Insert: flaA-GFP
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pTLP6; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:10447882
Comments: The ~1kb BamHI/SphI flaAGFP fragment from pflaG into the ~4.6kb BamHI/SphI fragment of the ColE1 replicon pTLP6.
Proper citation: RRID:Addgene_45476 Copy
Genetic Insert: endA::frt lambda pir
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:21306445
Comments: Use lambda pir strain for R6K ori replication. endA was deleted for cleaner and higher yield plasmid preps.
Proper citation: RRID:Addgene_45472 Copy
Species: Homo sapiens
Genetic Insert: PAI-RBP v2
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pCEFL; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11001948
Comments: Our lab isolated the protein based on it's ability to bind to a region of the PAI-1 (plasminogen activator inhibitor type 1) 3'UTR that we had determined to be involved in the cAMP regulation of mRNA stability. A search of the database revealed that the cDNA for the protein had been cloned by the German Genome Project and dubbed hypothetical protein. They kindly provided us with the clone, which we subcloned into pET-15b for expression of the protein and then into CMV-Flag for mammalian expression.
Subsequently, we discovered isoforms of the protein with or without 6 and/or 15 amino acid insertions. Variant 2 has the 6 amino acid insertion but lacks the 15 amino acid insertion.
Additional reference: Heaton, JH et al 2003 Thromb Haemost 89; 959-966.
Proper citation: RRID:Addgene_45505 Copy
Genetic Insert: Src (YF)-uniRapR-cerulean
Vector Backbone Description: Backbone Size:9773; Vector Backbone:pT2KHspGGFF (Asakawa K, Kawakami K. Dev Growth Differ. 2008); Vector Types:zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23569285
Comments: Please note that Addgene's sequencing results differ from the full plasmid sequence information from the depositing laboratory at bp# 3150, 3188, 3193, 3199, 3211, 3227, 3307, 5577 & 5578. These differences are not a concern for the function of the plasmid according to the depositing laboratory.
Proper citation: RRID:Addgene_45509 Copy
Species: Rhodopseudomonas palustris
Genetic Insert: iRFP720
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4038; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23770755
Proper citation: RRID:Addgene_45464 Copy
Species: Rhodopseudomonas palustris
Genetic Insert: iRFP670
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4055; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23770755
Proper citation: RRID:Addgene_45466 Copy
Species: Rhodopseudomonas palustris
Genetic Insert: iRFP713
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4038; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23770755
Comments: For more information on in vivo imaging, please see:
https://www.addgene.org/fluorescent_proteins/in_vivo/
Proper citation: RRID:Addgene_45465 Copy
Species: Mus musculus
Genetic Insert: PGC-1alpha Isoform1
Vector Backbone Description: Backbone Size:5424; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23217713
Comments: Note that Addgene NGS results identified a Q339K mutation relative to the canonical GenBank sequence. This mutation has not been reported to affect function.
Proper citation: RRID:Addgene_45501 Copy
Genetic Insert: frt-gent-frt
Vector Backbone Description: Backbone Marker:Datensko and Wanner; Vector Backbone:pR6K; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamycin
Defining Citation: PMID:21296953
Comments: frt-gent-frt cassette was cut out of pGEM-frt-gent-frt with EcoRI and ligated into EcoRI digested pR6K. Please note that the R6K ori may be in opposite orientation relative to cassette and annotation since it was not directionally cloned.
Proper citation: RRID:Addgene_45469 Copy
Species: Rhodopseudomonas palustris
Genetic Insert: iRFP720
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4067; Vector Backbone:pBAD/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23770755
Proper citation: RRID:Addgene_45460 Copy
Species: Rhodopseudomonas palustris
Genetic Insert: iRFP670
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4038; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23770755
Proper citation: RRID:Addgene_45462 Copy
Species: Rhodopseudomonas palustris
Genetic Insert: iRFP702
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4074; Vector Backbone:pBAD/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23770755
Proper citation: RRID:Addgene_45455 Copy
Species: Rhodopseudomonas palustris
Genetic Insert: iRFP670
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4012; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23770755
Comments: For more information on in vivo imaging, please see:
https://www.addgene.org/fluorescent_proteins/in_vivo/
Proper citation: RRID:Addgene_45457 Copy
Genetic Insert: hcRED
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pWzl-neo-flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23764425
Proper citation: RRID:Addgene_45458 Copy
Species: Synthetic
Genetic Insert: acrD
Vector Backbone Description: Vector Backbone:pBbA5k; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21556065
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_45441 Copy
Species: Homo sapiens
Genetic Insert: ATRX
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP C2; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24834375
Comments: The EcoRI restriction pattern is different between the plasmid described in the paper and the plasmid available from Addgene. This difference does not effect the function of the plasmid.
Proper citation: RRID:Addgene_45444 Copy
Species: Rattus norvegicus
Genetic Insert: NR1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12160751
Proper citation: RRID:Addgene_45446 Copy
Species: Rattus norvegicus
Genetic Insert: NR2A
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12160751
Proper citation: RRID:Addgene_45445 Copy
Species: Synthetic
Genetic Insert: Shal_1195
Vector Backbone Description: Vector Backbone:pBbA5k; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21556065
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_45438 Copy
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