Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Homo sapiens
Genetic Insert: PepT1
Vector Backbone Description: Backbone Size:3003; Vector Backbone:pBF; Vector Types:make RNA for Xenopus oocyte injection; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26320580
Proper citation: RRID:Addgene_73943 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: TK-kan
Vector Backbone Description: Vector Backbone:pFA6a-kanMX6; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27825338
Proper citation: RRID:Addgene_73894 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: natCX
Vector Backbone Description: Vector Backbone:pFA6a-natMX6; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27825338
Proper citation: RRID:Addgene_73893 Copy
Genetic Insert: TK-nat
Vector Backbone Description: Vector Backbone:pFA6a-natMX6; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27825338
Proper citation: RRID:Addgene_73895 Copy
Species: bacteria
Genetic Insert: bar and GUS cassettes
Vector Backbone Description: Backbone Size:7132; Vector Backbone:pPZP201; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:18256901
Comments: Discrepancies between Depositor's full sequence and Addgene's QC sequence are not of functional concern.
Proper citation: RRID:Addgene_73934 Copy
Species: Synthetic
Genetic Insert: bar
Vector Backbone Description: Backbone Size:7132; Vector Backbone:pPZP201; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:15034747
Comments: This vector is suitable for plant transformation and has been used as standard service empty vector control.
Proper citation: RRID:Addgene_73933 Copy
Genetic Insert: TK-nat
Vector Backbone Description: Vector Backbone:pFA6a-natMX6; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27825338
Proper citation: RRID:Addgene_73899 Copy
Species: Synthetic
Genetic Insert: bar
Vector Backbone Description: Backbone Size:7132; Vector Backbone:pPZP201; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:15034747
Comments: This vector is suitable hpRNA-mediated gene silencing in tissue (seed) specific manner.
Please see the Plant Transformation Core Facility website for more information on using this plasmid: http://plantsci.missouri.edu/muptcf/pzy101.html
The vector has following features:
1. It has two replicate origins that allow to replicate in both E. coli and Agrobacterium tumefaciens cells (at higher copy numbers).
2. It also has bom site that allows you to use tri-parental mating to mobilize the vector to Agrobacterium cells.
3. The aadA locus confers Spectinomycin (100 mg/L) or streptomycin (100 mg/L) resistance and thus enable to use either or both of these two antibiotics for the vector selection.
4. The multiple cloning site (MCS) adjacent to the double CaMV35S promoter will allow you to insert your cassette. This CaMV35S promoter is used to drive the bar ORF and, therefore, is not used in front to drive your gene of interest. You need to assemble your own expression cassette including promoter, gene coding sequence, and terminator, and then insert to anywhere between Hind III and EcoR I within the MCS.
Special notes:
1. Insert your gene expression cassette in such a way that your promoter is adjacent to the promoter driving the bar gene selectable marker. This is to prevent transcription read-through from your gene expression cassette.
2. In order to use the EcoR I site within the MCS, a minimal ratio of this enzyme versus DNA is recommended to avoid star activity. When HindIII and EcoRI are in combined use, even less of each enzyme is required, and reaction should be conducted in a large volume to avoid excessive enzymatic activity.
Proper citation: RRID:Addgene_73932 Copy
Genetic Insert: nls-Flamindo2
Vector Backbone Description: Backbone Size:5359; Vector Backbone:pcDNA3.1 (-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24959857
Proper citation: RRID:Addgene_73939 Copy
Species: Synthetic
Genetic Insert: Golden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin
Vector Backbone Description: Backbone Marker:Terrence Lai (this study); Backbone Size:1878; Vector Backbone:colE1 origin and chloramphenicol resistance; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27096716
Proper citation: RRID:Addgene_74091 Copy
Species: E. coli MG1655
Genetic Insert: lldR
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2044; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27096716
Proper citation: RRID:Addgene_74095 Copy
Species: E. coli MG1655
Genetic Insert: Bba_K822000 (L-Lactate promoter - E. coli MG1655)
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2044; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27096716
Proper citation: RRID:Addgene_74094 Copy
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:Modified pFastBacDual; Vector Types:Mammalian Expression, Bacmam, Baculovirus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27041595
Proper citation: RRID:Addgene_74099 Copy
Species: Synthetic
Genetic Insert: TL9 RBS
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2044; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27096716
Comments: This plasmid contains the TL9 RBS upstream of GFP.
Proper citation: RRID:Addgene_74098 Copy
Species: Rattus norvegicus
Genetic Insert: Psd95 beta isoform
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:9941; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16815335
Comments: Map: FUGW (w/o PacI)-HinDIII-SalI-XbaI-(p95b)-KpnI-SacII-PspOMI-SmaI-BamHI-(GFP)-BglII-BsrBI-EcoRV-HinDIII-ClaI
Psd95 shRNA targeting sequence GGACATCCAGGCACACAAG was changed to GGATATTCAAGCGCATAAG to make it resistant to the shRNA sequence.
Proper citation: RRID:Addgene_74015 Copy
Species: Rattus norvegicus
Genetic Insert: Psd95 shRNA
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:9941; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16815335
Comments: Map: FH95pUp95GW-SalI-XbaI-(p97a)-BamHI-(linker)-(EGFP)-BglII-BsrBI-EcoRV-HinDIII-ClaI
Proper citation: RRID:Addgene_74019 Copy
Genetic Insert: Psd95 shRNA
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:9941; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16815335
Comments: Map: FHUG+W-SalI-XbaI-BamHI-(EGFP+)-BspEI-BglII-XhoI-SacI-HinDIII-BstBI-EcoRI-(p97 I3)-EcoRV-HinDIII-ClaI-FHUG+W
Also associated with Liu, et al. J Neurophysiol. 2014 Feb;111(3):648-58. doi: 10.1152/jn.00262.2013 PMID 24225540.
Proper citation: RRID:Addgene_74017 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: mKO2
Vector Backbone Description: Backbone Size:4890; Vector Backbone:pRS316; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27479698
Proper citation: RRID:Addgene_74080 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: E2Crimson
Vector Backbone Description: Backbone Size:4890; Vector Backbone:pRS316; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27479698
Proper citation: RRID:Addgene_74082 Copy
Species: Photinus pyralis
Genetic Insert: Photinus pyralis firefly luciferin sulfotransferase
Vector Backbone Description: Backbone Marker:Produced in Jing-Ke Weng lab; Backbone Size:5350; Vector Backbone:pHis8-4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27227579
Comments: Expressed robustly in E.coli. Catalyzes sulfonation / desulfonation of firefly luciferin / sulfoluciferin with PAPS / PAP.
Proper citation: RRID:Addgene_74121 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the NIF Resources search. From here you can search through a compilation of resources used by NIF and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that NIF has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on NIF then you can log in from here to get additional features in NIF such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into NIF you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within NIF that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
Welcome to the Neuroscience Information Framework Project, designed to serve the biomedical research community. NIF maintains the largest searchable collection of neuroscience data, the largest catalog of biomedical resources, and the largest ontology for neuroscience on the web. We welcome all feedback and suggestions and are actively looking for resource providers to make their resources accessible through NIF. Learn about the tools available to help you share your data and discover a dynamic inventory of Web-based neuroscience resources.
