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Species: Synthetic
Genetic Insert: 4TC1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pPCR-Script Amp SK(+); Vector Types:PCR Cloning Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23242165
Proper citation: RRID:Addgene_41509 Copy
Species: Homo sapiens
Genetic Insert: hsDicer
Vector Backbone Description: Backbone Size:4790; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22546613
Proper citation: RRID:Addgene_41587 Copy
Species: Homo sapiens
Genetic Insert: hsDicer
Vector Backbone Description: Backbone Size:4790; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22546613
Proper citation: RRID:Addgene_41586 Copy
Species: Homo sapiens
Genetic Insert: hsDicer
Vector Backbone Description: Backbone Size:4790; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22546613
Proper citation: RRID:Addgene_41584 Copy
Vector Backbone Description: Backbone Size:4790; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22546613
Proper citation: RRID:Addgene_41583 Copy
Species: Homo sapiens
Genetic Insert: PKD1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21518865
Proper citation: RRID:Addgene_41580 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717241
Proper citation: RRID:Addgene_41614 Copy
Species: Homo sapiens
Genetic Insert: PKD1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21518865
Proper citation: RRID:Addgene_41579 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717241
Proper citation: RRID:Addgene_41611 Copy
Species: Homo sapiens
Genetic Insert: hPXR-LBD-T248E
Vector Backbone Description: Backbone Marker:ATCC (Item #77476); Backbone Size:5900; Vector Backbone:pSH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Human PXR ligand binding domain (LBD, aa 107-434) was cloned into vector pSH. Mutations were made and confirmed by sequencing.
Proper citation: RRID:Addgene_41619 Copy
Species: Homo sapiens
Genetic Insert: PKD1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21518865
Comments: 5' cloning site: EcoR1
3' cloning site: XhoI
Proper citation: RRID:Addgene_41575 Copy
Genetic Insert: 4xCSL binding sites
Vector Backbone Description: Backbone Marker:Dr. D. Towler (Washington University, St. Louis); Vector Backbone:RSV-TATA pGL2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11518718
Comments: The 4xCSL-luciferase reporter was constructed from the CBF1/pGL2-GLO TATA CAT plasmid, a gift from Dr. S. Speck. A fragment containing the multimerized high affinity CSL sites (4× CGTGGGAA) was excised by BamHI digest and ligated into a BglII/BamHI-digested RSV-TATA pGL2 vector, a gift from Dr. D. Towler. This modified vector has a TATA box from Rous sarcoma virus inserted into the pGL2-basic vector (Promega) to reduce the basal luciferase activity. Construct was sequenced for verification.
Alternate plasmid name: 4xCBS-luciferase
Proper citation: RRID:Addgene_41726 Copy
Species: Mus musculus
Genetic Insert: Hes5 Promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5597; Vector Backbone:pGL2-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9570950
Proper citation: RRID:Addgene_41724 Copy
Species: Mus musculus
Genetic Insert: Hes1 Promoter (-467 to +46)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5597; Vector Backbone:pGL2-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9570950
Comments: Alternate plasmid name: Hes1-Luc
Proper citation: RRID:Addgene_41723 Copy
Species: Thermoplasma volcanium
Genetic Insert: Tvo VMA intein
Vector Backbone Description: Backbone Marker:novagen; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22001202
Proper citation: RRID:Addgene_41689 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SEC63-mRFP
Vector Backbone Description: Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18812321
Comments: Please note that Addgene's quality control sequence shows several discrepancies with depositor's reference sequence. These are in vector region and should not affect function.
Proper citation: RRID:Addgene_41842 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6577; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23852599
Proper citation: RRID:Addgene_41841 Copy
Genetic Insert: RLuc110UGA
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4079; Vector Backbone:pRL-CMV; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19208811
Proper citation: RRID:Addgene_41729 Copy
Species: Homo sapiens
Genetic Insert: Dronpa145N-IntersectinDH-Dronpa145N-CAAX
Vector Backbone Description: Backbone Size:5370; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23139335
Proper citation: RRID:Addgene_41686 Copy
Species: Homo sapiens
Genetic Insert: apoptosis-associated speck-like protein containing CARD
Vector Backbone Description: Backbone Size:7332; Vector Backbone:pRP_LmCerulean; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23852599
Proper citation: RRID:Addgene_41840 Copy
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