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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pEF-Bos MAL Flag Resource Report Resource Website 1+ mentions |
RRID:Addgene_41554 | MAL | Homo sapiens | Ampicillin | PMID:12692549 | The depositing lab generated pEF-Bos MAL Flag from a human peripheral blood mononuclear cell (PBMC) complementary DNA library by PCR amplification and cloning. It was cloned with XhoI at the 5' end and BamHI at the 3' end. NotI can be used at the 3' end to excise the tagged CDS. The depositor's provided sequence includes some flanking EF-BOS sequence. The construct also contains a HIS tag. | Backbone Marker:Mizushima and Nagata (Osaka Bioscience Institute, Japan, 1990) (PMID: 1698283) ; Backbone Size:5685; Vector Backbone:pEF-Bos; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:46 | 2 | |
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pCI-ASC-HA Resource Report Resource Website 1+ mentions |
RRID:Addgene_41553 | ASC | Homo sapiens | Ampicillin | PMID:19158675 | Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:46 | 9 | ||
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pEF-Bos TRAM Flag Resource Report Resource Website 1+ mentions |
RRID:Addgene_41551 | TRAM | Homo sapiens | Ampicillin | PMID:14517278 | The depositing lab generated pEF-Bos Tram Flag from a human peripheral blood mononuclear cell (PBMC) complementary DNA library by PCR amplification and cloning. It was cloned with XhoI at the 5' end and BamHI at the 3' end. NotI can be used at the 3' end to excise the tagged CDS. The construct also contains a HIS tag. | Backbone Marker:Mizushima and Nagata (Osaka Bioscience Institute, Japan, 1990) (PMID: 1698283) ; Backbone Size:5685; Vector Backbone:pEF-Bos; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:46 | 1 | |
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pcDNA3.1(+)mGAT1-0-CFP Resource Report Resource Website |
RRID:Addgene_41664 | Mus musculus GABA transporter 1 | Mus musculus | Ampicillin | PMID:19948998 | To generate the fluorescent mutants mGAT10XFP and mGAT1XFP* through mGAT1XFP45, the wild-type mGAT1 open reading frame (ORF) was subcloned without its original stop codon into the HindIII and EcoRI sites of the pcDNA3.1(+) expression vector multiple cloning site (MCS). XFP ORFs were then subcloned downstream from and in frame with the mGAT1 ORF at the NotI and XbaI sites of the pcDNA3.1(+) MCS. This resulted in a 12–amino acid spacer between the end of the mGAT1 sequence and the beginning of the fluorophore. The depositors modified a method for the integration of PCR fragments without the use of restriction enzymes (Geiser et al., 2001) to add the final 3, 8, 20, 28, or 45 codons of the human GAT1 (hGAT1) ORF. These were amplified from a source plasmid using the proof-reading PfuTurbo Cx Hotstart polymerase with 5′ and 3′ extensions corresponding to the 20–22-nt regions that flanked the intended site of insertion, such that the PCR product integrated in-frame immediately after the fluorophore sequence when used as the primers in a subsequent QuikChange II XL mutagenesis PCR reaction. For mGAT1XFP*, the depositors simply added a GTC codon for Val after the fluorophore ORF. The attached image displays the protein sequences of the modified regions of mGAT1 for each fluorescent construct. mGAT10CFP and mGAT10YFP repeated the fusion design of mGAT10GFP but with the fluorophore exchanged as annotated. The three C-terminal residues of the mGAT0XFP fusions are -YKI-CO2−, which comprises a broadly defined consensus PDZ class II–interacting motif (X-φ-X-φ, where φ designates a hydrophobic residue and X any residue) (Sheng and Sala, 2001; Hung and Sheng, 2002). The depositors searched the Ensembl databases using Biomart (http://www.ebi.ac.uk/biomart) (Spudich et al., 2007) and applied the GO:0005886 “plasma membrane” cellular component filter. The search identified no known membrane proteins possessing the -YKI-CO2− C-terminal sequence. In the mGAT1XFP* constructs, the depositors defined the terminal residue P(0) more narrowly, changing the terminal isoleucine residue present in mGAT10XFP to a valine in mGAT1XFP*. The resulting C-terminal sequence, -YKV-CO2−, reconstituted a functional PDZ class II–interacting motif present in Ephrin B receptors, a class that relies on interactions with the PDZ domain–containing proteins for clustering (Torres et al., 1998; Brückner et al., 1999; Lin et al., 1999; Madsen et al., 2005). Other constructs in the C-terminal XFP fusion series, mGAT1XFP3, mGAT1XFP8, mGAT1XFP20, mGAT1XFP28, and mGAT1XFP45, had the most C-terminal 3, 8, 20, 28, or 45 residues of the hGAT1 appended after the mGAT1XFP fusion. The differences in nucleotide sequence between the hGAT1 and mGAT1 C termini were a useful source of positive identification when the depositors analyzed the clones during construction. PCR integration was applied to amplify and insert EYFP or ECFP directly between residues R565 and L566, I570 and Q571, or V577 and R578 of mGAT1 to generate the mGAT15xxXFP5xxCT constructs. The site of XFP insertion in GAT1 is highlighted in the nomenclatures for these constructs by residue numbers flanking the fluorophore, and the “CT” denotes that the insertion occurs within the C terminus. Please see the associated article for more detailed information regarding construct creation and usage. | Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | “Monomerizing” CFP A206K mutation; C-terminal hydrophobic isoleucine residue added after CFP | 2026-08-15 01:14:47 | 0 |
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EGFP-bSV-171-1792 Resource Report Resource Website |
RRID:Addgene_41661 | supervillin | Bos taurus | Kanamycin | PMID:10362542 | Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | amino acids 171-1792 | 2026-08-15 01:14:47 | 0 | |
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pETcon(I-LtrI) Resource Report Resource Website |
RRID:Addgene_41523 | I-LtrI | Leptographium truncatum | Ampicillin | Backbone Size:6145; Vector Backbone:pETcon; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:46 | 0 | |||
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G6PD/pRK5 Resource Report Resource Website 1+ mentions |
RRID:Addgene_41521 | glucose-6-phosphate dehydrogenase | Homo sapiens | Ampicillin | PMID:21336310 | Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:46 | 8 | ||
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TAL-BBL1-ID14 Resource Report Resource Website |
RRID:Addgene_41516 | Polylinker flanked by 2 Mva1269I cut sites | Synthetic | Kanamycin | PMID:23242165 | Backbone Marker:Clontech; Backbone Size:2638; Vector Backbone:pEGFP-N1Δ4728-2101; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:46 | 0 | ||
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TAL-4A1 Resource Report Resource Website |
RRID:Addgene_41512 | 4A1 | Synthetic | Ampicillin | PMID:23242165 | Backbone Size:2961; Vector Backbone:Custom backbone; Vector Types:PCR Cloning Vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:46 | 0 | ||
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TMEM216-mCherry Resource Report Resource Website |
RRID:Addgene_41633 | Transmembrane Protein 216 | Homo sapiens | Ampicillin | PMID:22282472 | See Table S6 of Supporting Online Material from associated publication for TMEM216 reference sequence. | Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:47 | 0 | |
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pFA6a-3HA-TRP1 Resource Report Resource Website |
RRID:Addgene_41599 | TRP1 | Saccharomyces cerevisiae | Ampicillin | PMID:9717241 | Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:47 | 0 | ||
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pCAGGS-Flag-hsDicer (Y971A/Y972A) Resource Report Resource Website 1+ mentions |
RRID:Addgene_41590 | hsDicer | Homo sapiens | Ampicillin | PMID:22546613 | Backbone Size:4790; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Y971A/Y972A | 2026-08-15 01:14:46 | 2 | |
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TAL-4TG1 Resource Report Resource Website |
RRID:Addgene_41510 | 4TG1 | Synthetic | Ampicillin | PMID:23242165 | Backbone Size:2961; Vector Backbone:Custom backbone; Vector Types:PCR Cloning Vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:46 | 0 | ||
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pFA6a-GFP(S65T)-TRP1 Resource Report Resource Website |
RRID:Addgene_41597 | TRP1 | Saccharomyces cerevisiae | Ampicillin | PMID:9717241 | Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:46 | 0 | ||
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pFA6a-TRP1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_41595 | TRP1 | Saccharomyces cerevisiae | Ampicillin | PMID:9717241 | Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:46 | 3 | ||
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TAL-4GT1 Resource Report Resource Website |
RRID:Addgene_41507 | 4GT1 | Synthetic | Ampicillin | PMID:23242165 | Backbone Size:2961; Vector Backbone:Custom backbone; Vector Types:PCR Cloning Vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:46 | 0 | ||
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TAL-4GC1 Resource Report Resource Website |
RRID:Addgene_41505 | 4GC1 | Synthetic | Ampicillin | PMID:23242165 | Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pPCR-Script Amp SK(+); Vector Types:PCR Cloning Vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:46 | 0 | ||
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TAL-4GA1 Resource Report Resource Website |
RRID:Addgene_41504 | 4GA1 | Synthetic | Ampicillin | PMID:23242165 | Backbone Size:2961; Vector Backbone:Custom backbone; Vector Types:PCR Cloning Vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:46 | 0 | ||
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pCAGGS-Flag-hsDicer (K70A) Resource Report Resource Website 1+ mentions |
RRID:Addgene_41589 | hsDicer | Homo sapiens | Ampicillin | PMID:22546613 | Backbone Size:4790; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | K70A | 2026-08-15 01:14:46 | 2 | |
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rbcLS-pMAL-2px Resource Report Resource Website |
RRID:Addgene_41621 | rbcL/rbcS | Synechococcus elongatus PCC 6301 | Ampicillin | PMID:16423843 | The rbcL/rbcS genes, which are adjacent in the Synechococcus PCC6301 chromosome, were PCR amplified together from whole cells using Vent polymerase and primers: 5'SrbcL (5'-GGGCCC[CATATG]CCCAAGACGCAATCTGCCGCAGG-3') and 3'SrbcS (5'-CCCGGG[GAGCTC]AGGCTTTAGTAGCGGCCGGGACG-3'). The rbcL/rbcS PCR product was cloned into pMAL-2px using restriction enzymes NdeI and SacI (bracketed), and sequenced to confirm its wild-type identity. Plasmid Features: Type Start End Name GENE 2 1420 rbcLS GENE 1511 1846 rbcS GENE 1862 2062 'malE-MCS-lacZa REGION 2047 2453 terminators GENE 2564 3424 ampR REGION 3466 3979 M13 ori REGION 4090 4678 pMB1 ori GENE 5108 5299 rop GENE 5807 6955 lacIQ REGION 7198 7225 Ptac | Backbone Marker:NEB; Backbone Size:6721; Vector Backbone:pMAL-p2x; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:47 | 0 |
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