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Species: Homo sapiens
Genetic Insert: Fos proto-oncogene, AP-1 transcription factor subunit
Vector Backbone Description: Vector Backbone:pFUW-tetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30517869
Proper citation: RRID:Addgene_125598 Copy
Species: Homo sapiens
Genetic Insert: PPAT
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30964683
Proper citation: RRID:Addgene_125620 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32183906
Comments: Similar to the situation with some other plasmids in this collection, the missing four nucleotides at the 5' end of the WPRE renders it unrecognizable to SnapGene, but the expression vector is functional.
Nevertheless, for sfGFP expression, the depositor recommends the updated versions of this plasmid, PB CAG-sfGFP (https://www.addgene.org/133568/) or PB-CA sfGFP (https://www.addgene.org/159094/), that can also be used without the piggyBac transposase.
Red fluorescent protein versions are also available: PB CAG-tdTomato (https://www.addgene.org/133569/) or PB-CA tdTomato (https://www.addgene.org/159095/).
Proper citation: RRID:Addgene_125747 Copy
Species: Synthetic
Genetic Insert: sfGFP-GSAx9-iCre-ERT2
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32183906
Comments: Linker between sfGFP and iCre-ERT2 is a clean GSAx9 sequence, similar in length to the mutated P2A sequence in the plasmid pCAG-sfGFP-iCre-ERT2 (#125749).
The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35). In short, it is not visible to the eyes, but can be detected with flow cytometry.
Similar to the situation with some other plasmids in this collection, the missing four nucleotides at the 5' end of the WPRE renders it unrecognizable to SnapGene, but the expression vector is functional.
Proper citation: RRID:Addgene_125748 Copy
Species: Other
Genetic Insert: BE4max-NG
Vector Backbone Description: Vector Backbone:pBT195; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31332326
Comments: Please see https://benchling.com/s/seq-auFYCwa9cwpOwF75PnyS for additional information.
Proper citation: RRID:Addgene_125617 Copy
Genetic Insert: PSD95.FingR-eGFP-CCR5TC
Vector Backbone Description: Backbone Size:4589; Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32674057
Proper citation: RRID:Addgene_125691 Copy
Genetic Insert: mScarlet-Gephyrin.FingR-IL2RGTC
Vector Backbone Description: Backbone Size:4589; Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32674057
Proper citation: RRID:Addgene_125695 Copy
Genetic Insert: PSD95.FingR-eGFP-CCR5TC
Vector Backbone Description: Backbone Size:4589; Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32674057
Proper citation: RRID:Addgene_125693 Copy
Species: Homo sapiens
Genetic Insert: AlkBH5(66-292)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31740367
Proper citation: RRID:Addgene_125732 Copy
Species: Danio rerio
Genetic Insert: bactin:QF2
Vector Backbone Description: Vector Backbone:pDestTol2pA3; Vector Types:zebrafish expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_125697 Copy
Species: Other
Genetic Insert: evoFERNY-BE4max-NG
Vector Backbone Description: Vector Backbone:pBT195; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31332326
Comments: Please see https://benchling.com/s/seq-yOkx507aEyhFduGQKQOz for additional information.
Proper citation: RRID:Addgene_125615 Copy
Species: Other
Genetic Insert: evoAPOBEC1-BE4max-NG
Vector Backbone Description: Vector Backbone:pBT195; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31332326
Comments: Please see https://benchling.com/s/seq-YMX6CoDtiqAsxyhvf3mx for additional information.
Proper citation: RRID:Addgene_125616 Copy
Species: Synthetic
Genetic Insert: SYK biosensor
Vector Backbone Description: Backbone Marker:Invitrogen, modified at Dr. Roger Tsien Lab in University of California, San Diego; Backbone Size:5505; Vector Backbone:pcDNA3.1’; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25541586
Proper citation: RRID:Addgene_125729 Copy
Species: Homo sapiens
Genetic Insert: PPAT
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30964683
Proper citation: RRID:Addgene_125680 Copy
Species: Homo sapiens
Genetic Insert: GART
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30964683
Comments: Please note that Addgene's quality control result found a 12bp deletion in the Neomycin Resistance cassette. The depositing lab has noted that this deletion will NOT affect plasmid function as described in their publication, but they have not tested for Neomycin selection directly. We suggest testing Neomycin selection if you need to generate stable cell lines.
Proper citation: RRID:Addgene_125681 Copy
Species: Homo sapiens
Genetic Insert: ATIC
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30964683
Proper citation: RRID:Addgene_125685 Copy
Species: Homo sapiens
Genetic Insert: GATA binding protein 2
Vector Backbone Description: Vector Backbone:pFUW-tetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30517869
Proper citation: RRID:Addgene_125600 Copy
Species: Other
Genetic Insert: streptomyces codon optimized spCas9, sgRNA casette, and ScaligD
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:25806970
Comments: Please note that an IS1 transposase sequence was found downstream of Cas9 during Addgene's quality control. The depositor noted that this sequence should NOT affect plasmid function.
Proper citation: RRID:Addgene_125688 Copy
Species: Other
Genetic Insert: streptomyces codon optimized spdCas9, sgRNA casette
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:25806970
Proper citation: RRID:Addgene_125687 Copy
Species: Homo sapiens
Genetic Insert: pT7-gRNA GFP expression, PYL-linker-T7 RNAPC, T7 RNAPN (N-29-1)-linker-ABI
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28809467
Proper citation: RRID:Addgene_125716 Copy
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