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Organism Name
E. coli [MG1655 bioB::kan].
RRID:WB-STRAIN:WBStrain00062723 RRID Copied  
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RRID:WB-STRAIN:WBStrain00062723
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Organism Information

URL: http://www.wormbase.org/db/get?name=WBStrain00062723

Proper Citation: RRID:WB-STRAIN:WBStrain00062723

Description: Escherichia coli with name E. coli [MG1655 bioB::kan]. from WB.

Species: Escherichia coli

Synonyms: E. coli [MG1655 bioB::kan].

Notes: Biotin auxotrophic E. coli strain is kan resistant and grows fine on LB. This mutant strain produces significantly less biotin and can therefore be used to reduce background in TurboID experiments. Also known as MG1655 bioB::kan and STL110 (J. Cronen, U. of Illinois). References: Sanchez AD & Feldman JL. STAR Protoc. 2021 Dec 2;2(4):100986. doi: 10.1016/j.xpro.2021.100986. PMID: 34927095. Ortega-Cuellar D., et al. J Nutrigenet Nutrigenomics. 2010;3(1):18-30. doi: 10.1159/000318054. PMID: 20798549|"Made_by: Ortega-Cuellar et al., 2010"

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Data and Source Information

Source: Integrated Animals

Source Database: WormBase (WB)