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URL: https://sites.wustl.edu/tetrahymena/finding-strains/
Proper Citation: RRID:TSC_SD02599
Description: Tetrahymena thermophila with name DRH1g∆∆ D3-11F from TSC.
Species: Tetrahymena thermophila
Notes: Homozygous homokaryon KO of DRH1 with neo4 cassette. Mates with DRH1g∆∆ C10-11F Gene is Dicer Related Helicase, sequence is fromTGD 69.m00174. Mating cells stop at mic selection after MeiosisII Gene is expressed at low level in starve and log, peak expression at 4 hr of conjugation. Somatic KO is complete, when mated they arrest after Meiosis II. Homozygous homokaryon KOs do not grow well. Needs Cd for pm resistance. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Affected Gene: DRH1 (TTHERM_00190830)
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Source: Tetrahymena Stock Center (RRID:SCR_008362)
Description: Centralized repository and distribution site for variety of Tetrahymena strains and species. Maintains diverse array of wild type, mutant, and genetically engineered strains of T. thermophila, the most commonly used laboratory species, and variety of other species derived from both laboratory maintained stocks and wild isolates. All stocks are stored in liquid nitrogen to maintain genetic integrity and prevent senescence. In addition to providing worldwide access to strains currently in collection, TSC continually upgrades collection by accepting deposition of newly developed laboratory strains and well characterized wild isolates collected from clearly defined natural sites.
URL: https://tetrahymena.vet.cornell.edu/
Source Database: TSC, Tetrahymena Stock Center
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