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URL: https://sites.wustl.edu/tetrahymena/finding-strains/
Proper Citation: RRID:TSC_SD01740
Description: Tetrahymena thermophila with name VI005 from TSC.
Species: Tetrahymena thermophila
Notes: The knockout also deletes 838bp of the 5' flanking region and introduces a frameshift mutation. The knockout has been confirmed by paromomycin resistance, but macronuclear allele homozygosity has not been verified. The neo4-KanR2 knockout cassette was generated by cloning fragments of the pNeo4 plasmid's neo4 cassette and the pUC4K plasmid's KanR2 cassette. The KanR2 gene was used for E. coli transformation and is not a selectable marker in Tetrahymena. Submitted by Robert Coyne, J. Craig Venter Institute
Affected Gene: LSD2 (TTHERM_00600670)
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Source: Tetrahymena Stock Center (RRID:SCR_008362)
Description: Centralized repository and distribution site for variety of Tetrahymena strains and species. Maintains diverse array of wild type, mutant, and genetically engineered strains of T. thermophila, the most commonly used laboratory species, and variety of other species derived from both laboratory maintained stocks and wild isolates. All stocks are stored in liquid nitrogen to maintain genetic integrity and prevent senescence. In addition to providing worldwide access to strains currently in collection, TSC continually upgrades collection by accepting deposition of newly developed laboratory strains and well characterized wild isolates collected from clearly defined natural sites.
URL: https://tetrahymena.vet.cornell.edu/
Source Database: TSC, Tetrahymena Stock Center
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