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URL: https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=124713544
Proper Citation: RRID:RGD_124713544
Description: Rattus norvegicus with name W-Cyp27b1em1Thka from RGD.
Species: Rattus norvegicus
Notes: This strain was established by injecting Jcl:Wistar embryo with CRISPR/Cas9 system to delete the cysteine at position 462 in exon 8, which is the 5th ligand of heme iron and an active center of Cyp27b1. The resulting mutation is a 25 amino acid deletion (75 bp deletion) in the target site. The mutant was maintained with CE-2 formula diet (CLEA Japan, Inc., Tokyo, Japan) containing 1.15% calcium and 2,100 IU vitamin D3/kg diet. Homozygotes Cyp27b1 mutants were maintained by mating of heterozygotes.
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Source: Integrated Animals
Source Database: Rat Genome Database (RGD)
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