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URL: https://fccl.ku.edu/
Proper Citation: University of Kansas Flow Cytometry Core Facility (RRID:SCR_028833)
Description: Core provides access to flow cytometry and cell sorting instrumentation and expertise. Services include cell sorting and multi-parametric analysis of individual cells in solution. Provides assistance in sample processing, data analysis, instrument training, software support, method and grant assistance, manuscript support, and consulting. BSL-2 facility is equipped with BD FACSymphony S6 and FACSAria Fusion cell sorters, a Cytek Aurora spectral flow cytometer, an Agilent NovoCyte Advanteon conventional flow cytometer, and other supplemental assay instrumentation (Bio-Rad QX600 ddPCR, C.T.L ImmunoSpot). The flow cytometry analyzers provide users with tube- and plate-based, conventional and spectral flow cytometry. The BD FACS instruments allow measurement and sorting of up to 6 resolved populations of cells simultaneously, based on up to 50 parameters of detection using 18 simultaneous fluorochromes. The facility is equipped to handle BSL-2 samples and perform aseptic and single cell sorting into tubes or 96-well plates. The facility provides instrument training for users who desire to become self-operators of the facility instruments. The FCC will equip CBID researchers with tools directly applicable to infectious disease research, such as identifying and characterizing infectious agents such as bacteria and parasites, quantification and sorting of cells infected with microbial pathogens, and assessing chemical probe efficacy against infectious agents.
Synonyms: KU Flow Cytometry Core, Lawrence, University of Kansas, KS
Resource Type: service resource, access service resource, core facility
Keywords: ABRF, flow cytometry services, cell sorting,
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