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Max Planck Institute of Biochemistry Protein Production Core Facility (RRID:SCR_025741)Copy Citation Copied
URL: https://www.biochem.mpg.de/protein_production
Proper Citation: Max Planck Institute of Biochemistry Protein Production Core Facility (RRID:SCR_025741)
Description: Facility for protein production. Provides services for protein expression and purification, assists with projects in designing strategies for high level expression of soluble proteins, provides vectors and strains. Offers clonning the gene of interest into expression vectors, rapid screening for optimal combinations of vectors, strains and expression conditions and producing protein of interest in E. coli, Pichia pastoris, mammalian and insect cells. Produces biomass at 1L to 10L scale using multiple bioreactor system for high cell density fermentation of bacteria and yeast cells and reusable bioreactor for cell culture. Proteins are then purified using standard techniques and chromatography systems. Quality control is routinely assessing protein identity, purity, stability and folding using set of different biochemical and biophysical methods.
Abbreviations: MPIB-PPCF
Synonyms: Protein Production Facility, Max Planck Institute of Biochemistry Protein Production Facility
Resource Type: service resource, core facility, access service resource
Keywords: protein production, protein expression, protein purification, expression vectors, E. coli, Pichia pastoris, mammalian cells, insect cells, bioreactor,
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