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Resource Name
PRIMEGENS
RRID:SCR_005474 RRID Copied      
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PRIMEGENS (RRID:SCR_005474)
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Resource Information

URL: http://primegens.org/

Proper Citation: PRIMEGENS (RRID:SCR_005474)

Description: A Web-based Tool for High-throughput Primer and Probe Design. The program has its different utilities available on its web server. A standalone version is also available. Algorithms: * SSPD - Sequence Specific Primer Design: to design primers for each of the specific sequences given by the user in the query input file against any alternate potential hybridization with any of the sequences given in the database input file. * PSPD - Probe Specific Primer Design: to design primers it selects the gene-specific fragments (probes) to design primer pairs for their PCR amplification. * FSPD Fragment Specific Primer Design: primer design algorithm used when there is a very long query sequence for which multiple primers are required for its amplification. * Check Binding Specificity * Probe Design Only: Probe design algorithm could be used to find sequence-specific probes, which doesn''t show any blast hit against database. Such probe design has been used for targeted sequencing like agilent sure-select technology with next-generation sequencing.

Abbreviations: PRIMEGENS

Synonyms: PRIMEGENS-w3, PRIMEGENS - High-throughput Primer and Probe Design, PRIMEGENSw3

Resource Type: analysis service resource, data analysis service, production service resource, service resource, software resource

Defining Citation: PMID:21415011, PMID:18579568, PMID:17951795, PMID:12424113

Keywords: primer design, high-throughput primer, probe, microarray, analysis, blast, hybridization, primer, probe design, dna methylation

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OMICtools

has parent organization

University of Missouri; Missouri; USA

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