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URL: http://antibodyregistry.org/AB_370380
Proper Citation: GeneTex Cat# GTX15289, RRID:AB_370380
Target Antigen: FLI1 antibody
Host Organism: rabbit
Clonality: polyclonal
Comments: Discontinued; manufacturer recommendations: IgG; IgG Immunoprecipitation; Immunohistochemistry; Immunohistochemistry - fixed; Western Blot; For IHC-P: Use at a dilution of 1:50. Perform enzymatic antigen retrieval before commencing with IHC staining protocol, by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. For IP: Use at a concentration of 2 µg/mg protein lysate. For WB: Use at a concentration of 2 µg/ml for 2hrs. Predicted molecular weight: 54 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot.
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No rating or validation information has been found for FLI1 antibody.
Discontinued
Discontinued at GeneTex
Source: Antibody Registry
dkNET can assist you in preparing authentication plans for antibodies to comply with the NIH Submission Policy. The information can be used while planning your experiments or submitting grant applications. The authentication plan for antibodies is based on methods suggested in "A proposal for validation of antibodies" (Uhlen M et. al., 2016), the guideline published in the Journal of Comparative Neurology (Saper C, 2005)(2), and the example " Authentication of of Key Biological and/or Chemical Resources "(Bandrowski A). For best practices for authenticating antibodies, check our Authentication Reports services.