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Maurice Elphick - Queen Mary University of London, UK Cat# ArCCK/SKR, RRID:AB_2891354Copy Citation Copied
URL: http://antibodyregistry.org/AB_2891354
Proper Citation: Maurice Elphick - Queen Mary University of London, UK Cat# ArCCK/SKR, RRID:AB_2891354
Target Antigen: CCK/SK-type receptor
Host Organism: guinea pig
Clonality: polyclonal
Comments: "To generate antibodies to ArCCK/SKR, a peptide corresponding to the C-terminal 15 amino-acids of ArCCK/SK-R with addition of an N-terminal reactive lysine residue was synthesized (KPSPTNYTNVSSDSSV, Peptide Protein Research Ltd, Fareham, UK). The peptide was conjugated to porcine thyroglobulin (Sigma-Aldrich, Gillingham, UK) as a carrier protein using 5% glutaraldehyde (Sigma-Aldrich, Gillingham, UK) in phosphate buffer (0.1 M; pH 7.2) and the conjugate was used for immunisation of a guinea pig (56-day protocol; Charles River Biologics, Romans, France). The antigen was emulsified in Freund’s complete adjuvant for primary immunisations (~100 nmol antigen peptide) and in Freund’s incomplete adjuvant for three booster immunisations (~50 nmol antigen peptide). The presence of antibodies to the antigen peptide in post-immunisation serum samples was assessed using an enzyme-linked immunosorbent assay (ELISA), in comparison with pre-immune serum"
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