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Greg M. Anderson Lab; University of Otago; New Zealand Cat# GA01, RRID:AB_2721114Copy Citation Copied
URL: http://antibodyregistry.org/AB_2721114
Proper Citation: Greg M. Anderson Lab; University of Otago; New Zealand Cat# GA01, RRID:AB_2721114
Target Antigen: GnRH precursor peptide
Host Organism: rabbit
Clonality: polyclonal
Comments: Rizwan MZ et al 2012 Endocrinology 153: 3770-3779 "Generation of new GnRH precursor peptide antisera (GA01 and GA02): GnRH precursor peptide antiserum was generated to a synthetic sequence corresponding to amino acids 23-39 of the mouse GnRH precursor peptide sequence (HWSYGLRPGGKRNTEHL) with Cys added to the N terminus and keyhole limpet hemocyanin as the conjugated carrier protein. This sequence, which overlaps with the sequence that encodes mature GnRH, was chosen based on predicted antigenicity. The antigen was mixed with Freund’s complete adjuvant (Sigma-Aldrich, Inc., St Louis, MO) and injected into a New Zealand white rabbit (200 µg sc) and a guinea pig (100 µg sc). Three booster injections in Freund’s incomplete adjuvant (Sigma-Aldrich) (rabbit: 100 µg sc; guinea pig: 50 µg sc) were administered at 2-weekly intervals. Two weeks after the final booster injection, the animals were exsanguinated, the serum harvested and sodium azide (0.1%) was added as a preservative. The rabbit and guinea pig antisera were designated GA01 and GA02 respectively."
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Source: Antibody Registry
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